Antiviral activity of Isatis indigotica root-derived clemastanin B against human and avian influenza A and B viruses in vitro

  • Authors:
    • Zifeng Yang
    • Yutao Wang
    • Zhaoguang Zheng
    • Suishan Zhao
    • Jin Zhao
    • Qing Lin
    • Chuyuan Li
    • Quan Zhu
    • Nanshan Zhong
  • View Affiliations

  • Published online on: February 7, 2013     https://doi.org/10.3892/ijmm.2013.1274
  • Pages: 867-873
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Abstract

Clemastanin B, 7S,8R,8'R-(-)-lariciresinol-4,4'-bis-O-β-D-glucopyranoside, is one of the major lignans extracted from Isatis indigotica root (IIR). In this study, the anti-influenza activities of clemastanin B were evaluated in vitro. Clemastanin B was found to inhibit different subtypes of human (H1N1, including swine-origin H1N1; H3N2 and influenza B) and avian influenza viruses (H6N2, H7N3, H9N2) at different magnitudes of activity (IC50 0.087-0.72 mg/ml) while this compound was inactive against respiratory syncytial virus (RSV), adenovirus 3 (ADV3), parainfluenza virus 3 (PIV3), enterovirus 71 (EV71) and human rhinovirus (HRV). An apparent virus titer reduction was detected when MDCK cells were treated with clemastanin B after viral infection, particularly at the early stage, and the ribonucleoprotein (RNP) of the influenza virus was retained in the nucleus after treatment with clemastanin B. These results demonstrated that clemastanin B targets viral endocytosis, uncoating or RNP export from the nucleus. Furthermore, treatment with clemastanin B did not easily result in the emergence of viral drug resistance. The effects of clemastanin B demonstrated in this study may promote the antiviral study of IIR, but additional studies are required to define the anti-influenza mechanism(s).
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April 2013
Volume 31 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yang Z, Wang Y, Zheng Z, Zhao S, Zhao J, Lin Q, Li C, Zhu Q and Zhong N: Antiviral activity of Isatis indigotica root-derived clemastanin B against human and avian influenza A and B viruses in vitro. Int J Mol Med 31: 867-873, 2013
APA
Yang, Z., Wang, Y., Zheng, Z., Zhao, S., Zhao, J., Lin, Q. ... Zhong, N. (2013). Antiviral activity of Isatis indigotica root-derived clemastanin B against human and avian influenza A and B viruses in vitro. International Journal of Molecular Medicine, 31, 867-873. https://doi.org/10.3892/ijmm.2013.1274
MLA
Yang, Z., Wang, Y., Zheng, Z., Zhao, S., Zhao, J., Lin, Q., Li, C., Zhu, Q., Zhong, N."Antiviral activity of Isatis indigotica root-derived clemastanin B against human and avian influenza A and B viruses in vitro". International Journal of Molecular Medicine 31.4 (2013): 867-873.
Chicago
Yang, Z., Wang, Y., Zheng, Z., Zhao, S., Zhao, J., Lin, Q., Li, C., Zhu, Q., Zhong, N."Antiviral activity of Isatis indigotica root-derived clemastanin B against human and avian influenza A and B viruses in vitro". International Journal of Molecular Medicine 31, no. 4 (2013): 867-873. https://doi.org/10.3892/ijmm.2013.1274