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International Journal of Molecular Medicine
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April 2013 Volume 31 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

ACF7 regulates colonic permeability

  • Authors:
    • Yong Liang
    • Chenzhang Shi
    • Jun Yang
    • Hongqi Chen
    • Yang Xia
    • Peng Zhang
    • Feng Wang
    • Huazhong Han
    • Huanlong Qin
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, The Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200233, P.R. China
  • Pages: 861-866
    |
    Published online on: February 21, 2013
       https://doi.org/10.3892/ijmm.2013.1284
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Abstract

Colonic paracellular permeability is regulated by various factors, including dynamics of the cytoskeleton. Recently, ACF7 has been found to play a critical role in cytoskeletal dynamics as an essential integrator. To elucidate the physiological importance of ACF7 and paracellular permeability, we conditionally knocked out ACF7 in the intestinal mucosa of mice. Histopathological findings indicated that ACF7 deficiency resulted in significant interstitial proliferation and columnar epithelial cell rearrangement. Decreased colonic paracellular permeability was detected using a Ussing chamber and the FITC-inulin method. In order to clarify the underlying mechanism, we further analyzed the expression levels of three important tight junction proteins. Downregulation of ZO-1, occludin and claudin-1 was identified. Immunofluorescence provided strong evidence that ZO-1, occludin and claudin-1 were weakly stained. We hypothesized that ACF7 regulates cytoskeleton dynamics to alter mucosal epithelial arrangement and colonic paracellular permeability.
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Copy and paste a formatted citation
Spandidos Publications style
Liang Y, Shi C, Yang J, Chen H, Xia Y, Zhang P, Wang F, Han H and Qin H: ACF7 regulates colonic permeability. Int J Mol Med 31: 861-866, 2013.
APA
Liang, Y., Shi, C., Yang, J., Chen, H., Xia, Y., Zhang, P. ... Qin, H. (2013). ACF7 regulates colonic permeability. International Journal of Molecular Medicine, 31, 861-866. https://doi.org/10.3892/ijmm.2013.1284
MLA
Liang, Y., Shi, C., Yang, J., Chen, H., Xia, Y., Zhang, P., Wang, F., Han, H., Qin, H."ACF7 regulates colonic permeability". International Journal of Molecular Medicine 31.4 (2013): 861-866.
Chicago
Liang, Y., Shi, C., Yang, J., Chen, H., Xia, Y., Zhang, P., Wang, F., Han, H., Qin, H."ACF7 regulates colonic permeability". International Journal of Molecular Medicine 31, no. 4 (2013): 861-866. https://doi.org/10.3892/ijmm.2013.1284
Copy and paste a formatted citation
x
Spandidos Publications style
Liang Y, Shi C, Yang J, Chen H, Xia Y, Zhang P, Wang F, Han H and Qin H: ACF7 regulates colonic permeability. Int J Mol Med 31: 861-866, 2013.
APA
Liang, Y., Shi, C., Yang, J., Chen, H., Xia, Y., Zhang, P. ... Qin, H. (2013). ACF7 regulates colonic permeability. International Journal of Molecular Medicine, 31, 861-866. https://doi.org/10.3892/ijmm.2013.1284
MLA
Liang, Y., Shi, C., Yang, J., Chen, H., Xia, Y., Zhang, P., Wang, F., Han, H., Qin, H."ACF7 regulates colonic permeability". International Journal of Molecular Medicine 31.4 (2013): 861-866.
Chicago
Liang, Y., Shi, C., Yang, J., Chen, H., Xia, Y., Zhang, P., Wang, F., Han, H., Qin, H."ACF7 regulates colonic permeability". International Journal of Molecular Medicine 31, no. 4 (2013): 861-866. https://doi.org/10.3892/ijmm.2013.1284
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