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International Journal of Molecular Medicine
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Article

Anticancer effects of sodium butyrate on hepatocellular carcinoma cells in vitro

  • Authors:
    • Hong-Gang Wang
    • Xiao-Dan Huang
    • Peng Shen
    • Lu-Rong Li
    • Hui-Ting Xue
    • Guo-Zhong Ji
  • View Affiliations / Copyright

    Affiliations: Institute of Digestive Endoscopy and Medical Center for Digestive Diseases, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210011, P.R. China, Department of Gastroenterology, The First People's Hospital of Kunshan, Kunshan, Jiangsu 215300, P.R. China
  • Pages: 967-974
    |
    Published online on: February 22, 2013
       https://doi.org/10.3892/ijmm.2013.1285
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Abstract

In the present study, we investigated the anticancer effects of sodium butyrate (NaBu) on hepatocellular carcinoma (HCC) cells in vitro. As a histone deacetylase (HDAC) inhibitor, NaBu upregulated Ac-H3 and inhibited HDAC4 protein expression in a time- and dose-dependent manner. MTT assays showed that treatment with NaBu at high concentrations significantly inhibited the growth of various HCC cells. Exposure to NaBu for 24 h induced cell cycle arrest in the SMMC-7721 and HepG2 cells. NaBu also induced the apoptosis of SMMC‑7721 cells. The expression levels of cell cycle- and apoptosis-related proteins were further investigated by western blot analysis using specific antibodies. The results provided a possible mechanism responsible for the inhibitory effects of NaBu on the growth of HCC cells. To further analyze the role of NaBu in cell migration, wound healing and Transwell assays were performed, indicating that NaBu significantly inhibits cell migration/invasion in HCC cells. Transforming growth factor-β1 (TGF-β1)-induced epithelial to mesenchymal transition (EMT) has been associated with tumor cell migration and invasion. The EMT markers, E-cadherin, vimentin and N-cadherin, were regulated by TGF-β1, while NaBu inhibited this process in which HDAC4 and matrix metalloproteinase (MMP)7 may be involved. Based on our findings, we propose that NaBu may be useful as an anticancer drug for HCC therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Wang H, Huang X, Shen P, Li L, Xue H and Ji G: Anticancer effects of sodium butyrate on hepatocellular carcinoma cells in vitro. Int J Mol Med 31: 967-974, 2013.
APA
Wang, H., Huang, X., Shen, P., Li, L., Xue, H., & Ji, G. (2013). Anticancer effects of sodium butyrate on hepatocellular carcinoma cells in vitro. International Journal of Molecular Medicine, 31, 967-974. https://doi.org/10.3892/ijmm.2013.1285
MLA
Wang, H., Huang, X., Shen, P., Li, L., Xue, H., Ji, G."Anticancer effects of sodium butyrate on hepatocellular carcinoma cells in vitro". International Journal of Molecular Medicine 31.4 (2013): 967-974.
Chicago
Wang, H., Huang, X., Shen, P., Li, L., Xue, H., Ji, G."Anticancer effects of sodium butyrate on hepatocellular carcinoma cells in vitro". International Journal of Molecular Medicine 31, no. 4 (2013): 967-974. https://doi.org/10.3892/ijmm.2013.1285
Copy and paste a formatted citation
x
Spandidos Publications style
Wang H, Huang X, Shen P, Li L, Xue H and Ji G: Anticancer effects of sodium butyrate on hepatocellular carcinoma cells in vitro. Int J Mol Med 31: 967-974, 2013.
APA
Wang, H., Huang, X., Shen, P., Li, L., Xue, H., & Ji, G. (2013). Anticancer effects of sodium butyrate on hepatocellular carcinoma cells in vitro. International Journal of Molecular Medicine, 31, 967-974. https://doi.org/10.3892/ijmm.2013.1285
MLA
Wang, H., Huang, X., Shen, P., Li, L., Xue, H., Ji, G."Anticancer effects of sodium butyrate on hepatocellular carcinoma cells in vitro". International Journal of Molecular Medicine 31.4 (2013): 967-974.
Chicago
Wang, H., Huang, X., Shen, P., Li, L., Xue, H., Ji, G."Anticancer effects of sodium butyrate on hepatocellular carcinoma cells in vitro". International Journal of Molecular Medicine 31, no. 4 (2013): 967-974. https://doi.org/10.3892/ijmm.2013.1285
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