Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
May 2013 Volume 31 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May 2013 Volume 31 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Silencing Bmi-1 expression by RNA interference suppresses the growth of laryngeal carcinoma cells

  • Authors:
    • Xiao-Bao Yao
    • Xiao-Xia Wang
    • Hui Liu
    • Shao-Qiang Zhang
    • Hong-Liang Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Otolaryngology, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, P.R. China
  • Pages: 1262-1272
    |
    Published online on: March 20, 2013
       https://doi.org/10.3892/ijmm.2013.1312
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of this study was to investigate the effect of a B-cell-specific MLV integration site-1 (Bmi-1) RNA interference (RNAi) expression vector on the proliferation and invasiveness of laryngeal carcinoma. We constructed a lentiviral vector expressing Bmi-1-specific short hairpin RNA (shRNA), and transfected it into HEp-2 cells. Bmi-1 gene expression was detected by real-time RT-PCR and western blot analysis. We used flow cytometry and TUNEL assay to analyze the apoptosis of transfected cells, and examined cellular growth in vitro by MTT assay. We established an animal model and evaluated the therapeutic effects of small interfering RNA (siRNA) against Bmi-1. siRNA against Bmi-1 significantly knocked down Bmi-1 expression in HEp-2 cells, induced cell cycle arrest at the G1 phase, inhibited cell proliferation and promoted cell apoptosis. Lentiviral Bmi-1-shRNA vector transfection also significantly reduced cell migration. The formation and growth rate of xenograft tumors in mice transfected with siRNA against Bmi-1 was significantly reduced. The loss of mitochondrial membrane potential, the release of cytochrome c from the mitochondria into the cytosol, and the increased activity of caspase-3, -8 and -9 occurred concomitantly with the inhibition of Bmi-1. Our data indicate that siRNA against Bmi-1 significantly suppresses tumor growth and induces apoptosis in vitro and in vivo.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

Figure 10

Figure 11

Figure 12

View References

1 

Marioni G, Marchese-Ragona R, Cartei G, Marchese F and Staffieri A: Current opinion in diagnosis and treatment of laryngeal carcinoma. Cancer Treat Rev. 32:504–515. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Papadas TA, Alexopoulos EC, Mallis A, Jelastopulu E, Mastronikolis NS and Goumas P: Survival after laryngectomy: a review of 133 patients with laryngeal carcinoma. Eur Arch Otorhinolaryngol. 267:1095–1101. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Sun YN, Liu M, Yang B, Li B and Lu J: Role of siRNA silencing of MMP-2 gene on invasion and growth of laryngeal squamous cell carcinoma. Eur Arch Otorhinolaryngol. 265:1385–1391. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Zhao JX and Xie XL: Regulation of gene expression in laryngeal carcinama by microRNAs. Int J Pathol Clin Med. 32:222–225. 2012.

5 

Srivastava R: Apoptosis, Cell Signaling, and Human Diseases Molecular Mechanisms. 1. Humana Press; Totowa: 2007

6 

Bea S, Tort F, Pinyol M, et al: BMI-1 gene amplification and overexpression in hematological malignancies occur mainly in mantle cell lymphomas. Cancer Res. 61:2409–2412. 2001.

7 

Bhattacharyya J, Mihara K, Ohtsubo M, et al: Overexpression of BMI-1 correlates with drug resistance in B-cell lymphoma cells through the stabilization of survivin expression. Cancer Sci. 103:34–41. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Sawa M, Yamamoto K, Yokozawa T, et al: BMI-1 is highly expressed in M0-subtype acute myeloid leukemia. Int J Hematol. 82:42–47. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Kimura M, Takenobu H, Akita N, et al: Bmi1 regulates cell fate via tumor suppressor WWOX repression in small-cell lung cancer cells. Cancer Sci. 102:983–990. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Kim JH, Yoon SY, Kim CN, et al: The Bmi-1 oncoprotein is overexpressed in human colorectal cancer and correlates with the reduced p16INK4a/p14ARF proteins. Cancer Lett. 203:217–224. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Glinsky GV, Berezovska O and Glinskii AB: Microarray analysis identifies a death-from-cancer signature predicting therapy failure in patients with multiple types of cancer. J Clin Invest. 115:1503–1521. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Song W, Tao K, Li H, et al: Bmi1 is related to proliferation, survival and poor prognosis in pancreatic cancer. Cancer Sci. 101:1754–1760. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Yao X, Wang X, Zhang S and Zhu H: Effects of Bmi-1 RNAi gene on laryngeal carcinoma Hep-2 cells. Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 26:550–557. 2012.(In Chinese).

14 

Kang MK, Kim RH, Kim SJ, et al: Elevated Bmi-1 expression is associated with dysplastic cell transformation during oral carcinogenesis and is required for cancer cell replication and survival. Br J Cancer. 96:126–133. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Song LB, Zeng MS, Liao WT, et al: Bmi-1 is a novel molecular marker of nasopharyngeal carcinoma progression and immortalizes primary human nasopharyngeal epithelial cells. Cancer Res. 66:6225–6232. 2006. View Article : Google Scholar

16 

Glinsky GV: Death-from-cancer signatures and stem cell contribution to metastatic cancer. Cell Cycle. 4:1171–1175. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Hoenerhoff MJ, Chu I, Barkan D, et al: BMI1 cooperates with H-RAS to induce an aggressive breast cancer phenotype with brain metastases. Oncogene. 28:3022–3032. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Xu CR, Lee SS, Ho C, et al: Bmi1 functions as an oncogene independent of Ink4A/Arf repression in hepatic carcinogenesis. Mol Cancer Res. 7:1937–1945. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Elbashir SM, Harborth J, Lendeckel W, et al: Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 411:494–498. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Mittal V: Improving the efficiency of RNA interference in mammals. Nat Rev Genet. 5:355–365. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Moffat J and Sabatini DM: Building mammalian signalling pathways with RNAi screens. Nat Rev Mol Cell Biol. 7:177–187. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Kim DH and Rossi JJ: Strategies for silencing human diseaseusing RNA interference. Nat Rev Genet. 8:173–184. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Huang XZ and Wang JB: Practical otolaryngology. People’s Medical Publishing House; Beijing: pp. 502–503. 1998

24 

Tu Gy, Tang PZ and Jia CY: Horizontovertical laryngectomy for supraglottic carcinoma. Otolaryngol Head Neck Surg. 117:280–286. 1997. View Article : Google Scholar : PubMed/NCBI

25 

Sessions DG, Lenox J, Spector GJ, et al: Management of T3N0M0 glottic carcinoma: therapeutic outcomes. Laryngoscope. 112:1281–1288. 2002. View Article : Google Scholar : PubMed/NCBI

26 

Liu YZ, Tang PZ, Qi YF and Xu Z: The management of stomal recurrence after laryngectomy. Zhonghua Er Bi Yan Hou Ke Za Zhi. 37:380–383. 2002.(In Chinese).

27 

Ringrose L and Paro R: Epigenetic regulation of cellular memory by the Polycomb and Trithorax group proteins. Annu Rev Genet. 38:413–443. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Raaphorst FM: Deregulated expression of Polycomb-group oncogenes in human malignant lymphomas and epithelial tumors. Hum Mol Genet. 14:R93–R100. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Kikuchi J, Kinoshita I, Shimizu Y, et al: Distinctive expression of the polycomb group proteins Bmi1 polycomb ring finger oncogene and enhancer of zeste homolog 2 in nonsmall cell lung cancers and their clinical and clinicopathologic significance. Cancer. 116:3015–3024. 2010. View Article : Google Scholar

30 

Yao XB, Wang XX, Zhang SQ, Yan LY and Zhu HL: Association of Bmi-1 mRNA overexpression with differentiation, metastasis and prognosis of laryngocarcinoma. Xi’An Jiaotong Daxue Xuebao. 32:246–249. 2011.(In Chinese).

31 

Wang YX, Wei YM, Wang XH and Zhu XY: Construction of eukaryotic expression vector targeting Bmi-1 and its influences on proliferation of SW480 cells. Xian Dai Sheng Wu Yi Xue Jin Zhan. 9:2270–2272. 2009.(In Chinese).

32 

Wang ZX, Lu BB, Yang JS, Wang KM and De W: Adenovirus-mediated siRNA targeting c-Met inhibits proliferation and invasion of small-cell lung cancer (SCLC) cells. J Surg Res. 171:127–135. 2011. View Article : Google Scholar : PubMed/NCBI

33 

Li PH, Wang HJ, Liu W and Xu XG: PRDM1 gene expression in laryngeal carcinoma and its clinical significance. Head Neck Surg. 24:179–180. 2010.

34 

Pirrotta V: Polycombing the genome: PcG, trxG, and chromatin silencing. Cell. 93:333–336. 1998. View Article : Google Scholar : PubMed/NCBI

35 

Orlando V: Polycomb, epigenomes, and control of cell identity. Cell. 112:599–606. 2003. View Article : Google Scholar : PubMed/NCBI

36 

Jacobs JJ, Kieboom K, Marino S, Depinho RA and van Lohuizen M: The oncogene and Polycomb-group gene bmi-1 regulates cell proliferation and senescence through the ink4a locus. Nature. 397:164–168. 1999. View Article : Google Scholar : PubMed/NCBI

37 

Itahana K, Zou Y, Itahana Y, et al: Control of the replicative life span of human fibroblasts by p16 and the polycomb protein Bmi-1. Mol Cell Biol. 23:389–401. 2003. View Article : Google Scholar : PubMed/NCBI

38 

Dimri GP: What has senescence got to do with cancer? Cancer Cell. 7:505–512. 2005. View Article : Google Scholar : PubMed/NCBI

39 

Xu Z, Liu H, Lv X, Liu Y, Li S and Li H: Knockdown of the Bmi-1 oncogene inhibits cell proliferation and induces cell apoptosis and is involved in the decrease of Akt phosphorylation in the human breast carcinoma cell line MCF-7. Oncol Rep. 25:409–418. 2011.PubMed/NCBI

40 

Liu WL, Guo XZ, Zhang LJ, et al: Prognostic relevance of Bmi-1 expression and autoantibodies in esophageal squamous cell carcinoma. BMC Cancer. 10:4672010. View Article : Google Scholar : PubMed/NCBI

41 

Abdouh M, Facchino S, Chatoo W, et al: BMI1 sustains human glioblastoma multiforme stem cell renewal. J Neurosci. 29:8884–8896. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yao X, Wang X, Liu H, Zhang S and Zhu H: Silencing Bmi-1 expression by RNA interference suppresses the growth of laryngeal carcinoma cells. Int J Mol Med 31: 1262-1272, 2013.
APA
Yao, X., Wang, X., Liu, H., Zhang, S., & Zhu, H. (2013). Silencing Bmi-1 expression by RNA interference suppresses the growth of laryngeal carcinoma cells. International Journal of Molecular Medicine, 31, 1262-1272. https://doi.org/10.3892/ijmm.2013.1312
MLA
Yao, X., Wang, X., Liu, H., Zhang, S., Zhu, H."Silencing Bmi-1 expression by RNA interference suppresses the growth of laryngeal carcinoma cells". International Journal of Molecular Medicine 31.5 (2013): 1262-1272.
Chicago
Yao, X., Wang, X., Liu, H., Zhang, S., Zhu, H."Silencing Bmi-1 expression by RNA interference suppresses the growth of laryngeal carcinoma cells". International Journal of Molecular Medicine 31, no. 5 (2013): 1262-1272. https://doi.org/10.3892/ijmm.2013.1312
Copy and paste a formatted citation
x
Spandidos Publications style
Yao X, Wang X, Liu H, Zhang S and Zhu H: Silencing Bmi-1 expression by RNA interference suppresses the growth of laryngeal carcinoma cells. Int J Mol Med 31: 1262-1272, 2013.
APA
Yao, X., Wang, X., Liu, H., Zhang, S., & Zhu, H. (2013). Silencing Bmi-1 expression by RNA interference suppresses the growth of laryngeal carcinoma cells. International Journal of Molecular Medicine, 31, 1262-1272. https://doi.org/10.3892/ijmm.2013.1312
MLA
Yao, X., Wang, X., Liu, H., Zhang, S., Zhu, H."Silencing Bmi-1 expression by RNA interference suppresses the growth of laryngeal carcinoma cells". International Journal of Molecular Medicine 31.5 (2013): 1262-1272.
Chicago
Yao, X., Wang, X., Liu, H., Zhang, S., Zhu, H."Silencing Bmi-1 expression by RNA interference suppresses the growth of laryngeal carcinoma cells". International Journal of Molecular Medicine 31, no. 5 (2013): 1262-1272. https://doi.org/10.3892/ijmm.2013.1312
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team