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International Journal of Molecular Medicine
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Article

Hydrogen-rich medium suppresses the generation of reactive oxygen species, elevates the Bcl-2/Bax ratio and inhibits advanced glycation end product-induced apoptosis

  • Authors:
    • Hong Jiang
    • Pan Yu
    • De-Hui Qian
    • Zhe-Xue Qin
    • Xue-Jun Sun
    • Jie Yu
    • Lan Huang
  • View Affiliations / Copyright

    Affiliations: Institute of Cardiovascular Diseases of PLA, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P.R. China, Department of Burns and Plastic Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu 210002, P.R. China, Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, P.R. China
  • Pages: 1381-1387
    |
    Published online on: April 5, 2013
       https://doi.org/10.3892/ijmm.2013.1334
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Abstract

The purpose of the present study was to determine whether using hydrogen-rich medium (HRM) to increase hydrogen levels in endothelial cells (ECs) protects ECs from apoptosis induced by advanced glycation end products (AGEs). The thoracic aorta was removed from 2-3-year-old Sprague-Dawley rats, and ECs were isolated and cultured. After culturing ECs in the presence of AGEs and/or with HRM for 24 h, Annexin V/7-AAD and TUNEL staining were carried out to detect apoptosis. Intracellular ROS were detected by fluorescent probe and quantified by flow cytometry. The expression of antioxidative enzymes (superoxide dismutase, glutathione peroxidase) was determined by real-time PCR analysis and enzymatic assay. The relative expression levels of Bcl-2 and Bax were analyzed by western blotting. The addition of AGEs increased the apoptosis of ECs in a concentration-dependent manner and HRM reduced the AGE (400 µg/ml)-induced apoptosis from 21.61±2.52 to 11.32±1.75%. HRM also significantly attenuated the AGE-induced intracellular ROS induction and decrease in the expression of antioxidative enzymes. In conclusion, hydrogen exhibits significant protective effects against AGE-induced EC injury possibly through reducing ROS generation, intracellular antioxidant enzyme system protection and elevation of the Bcl-2/Bax ratio.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang H, Yu P, Qian D, Qin Z, Sun X, Yu J and Huang L: Hydrogen-rich medium suppresses the generation of reactive oxygen species, elevates the Bcl-2/Bax ratio and inhibits advanced glycation end product-induced apoptosis. Int J Mol Med 31: 1381-1387, 2013.
APA
Jiang, H., Yu, P., Qian, D., Qin, Z., Sun, X., Yu, J., & Huang, L. (2013). Hydrogen-rich medium suppresses the generation of reactive oxygen species, elevates the Bcl-2/Bax ratio and inhibits advanced glycation end product-induced apoptosis. International Journal of Molecular Medicine, 31, 1381-1387. https://doi.org/10.3892/ijmm.2013.1334
MLA
Jiang, H., Yu, P., Qian, D., Qin, Z., Sun, X., Yu, J., Huang, L."Hydrogen-rich medium suppresses the generation of reactive oxygen species, elevates the Bcl-2/Bax ratio and inhibits advanced glycation end product-induced apoptosis". International Journal of Molecular Medicine 31.6 (2013): 1381-1387.
Chicago
Jiang, H., Yu, P., Qian, D., Qin, Z., Sun, X., Yu, J., Huang, L."Hydrogen-rich medium suppresses the generation of reactive oxygen species, elevates the Bcl-2/Bax ratio and inhibits advanced glycation end product-induced apoptosis". International Journal of Molecular Medicine 31, no. 6 (2013): 1381-1387. https://doi.org/10.3892/ijmm.2013.1334
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang H, Yu P, Qian D, Qin Z, Sun X, Yu J and Huang L: Hydrogen-rich medium suppresses the generation of reactive oxygen species, elevates the Bcl-2/Bax ratio and inhibits advanced glycation end product-induced apoptosis. Int J Mol Med 31: 1381-1387, 2013.
APA
Jiang, H., Yu, P., Qian, D., Qin, Z., Sun, X., Yu, J., & Huang, L. (2013). Hydrogen-rich medium suppresses the generation of reactive oxygen species, elevates the Bcl-2/Bax ratio and inhibits advanced glycation end product-induced apoptosis. International Journal of Molecular Medicine, 31, 1381-1387. https://doi.org/10.3892/ijmm.2013.1334
MLA
Jiang, H., Yu, P., Qian, D., Qin, Z., Sun, X., Yu, J., Huang, L."Hydrogen-rich medium suppresses the generation of reactive oxygen species, elevates the Bcl-2/Bax ratio and inhibits advanced glycation end product-induced apoptosis". International Journal of Molecular Medicine 31.6 (2013): 1381-1387.
Chicago
Jiang, H., Yu, P., Qian, D., Qin, Z., Sun, X., Yu, J., Huang, L."Hydrogen-rich medium suppresses the generation of reactive oxygen species, elevates the Bcl-2/Bax ratio and inhibits advanced glycation end product-induced apoptosis". International Journal of Molecular Medicine 31, no. 6 (2013): 1381-1387. https://doi.org/10.3892/ijmm.2013.1334
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