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Article

Astragalus polysaccharide induces anti-inflammatory effects dependent on AMPK activity in palmitate-treated RAW264.7 cells

  • Authors:
    • Jinzhi Lu
    • Xiong Chen
    • Yingying Zhang
    • Jian Xu
    • Lianglu Zhang
    • Zhi Li
    • Wanhong Liu
    • Jingping Ouyang
    • Song Han
    • Xiaohua He
  • View Affiliations / Copyright

    Affiliations: School of Basic Medical Sciences, Wuhan University, Wuhan 430071, P.R. China
  • Pages: 1463-1470
    |
    Published online on: April 5, 2013
       https://doi.org/10.3892/ijmm.2013.1335
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Abstract

Astragalus polysaccharide (APS) has been reported to increase insulin sensitization and to ameliorate diabetes in animal models, and studies have demonstrated that this effect may be correlated with its anti-inflammatory roles in vivo and in vitro. However, the potential pharmacological mechanisms of APS in anti-inflammatory regulation are still poorly understood. Herein, RAW264.7 cells treated with APS showed anti-inflammatory effects. Interleukin (IL)-10 protein levels and expression of most of the anti-inflammatory genes, including IL-10, macrophage mannose receptor (MMR), arginase, Dectin-1, YM-1 and YM-2, were significantly increased after treatment with APS for 24 h. Furthermore, to determine whether APS plays a potential role in RAW264.7 cell inflammation, we pretreated RAW264.7 cells with APS in the presence of palmitate. The results showed that APS markedly recovered the impairment of AMPK activity induced by palmitate. Furthermore, APS induced IL-10 protein production and anti-inflammatory gene expression of IL-10, MMR, Dectin-1, arginase, YM-1 and YM-2. Additionally, APS inhibited IL-1β protein production and expression of most of the pro-inflammatory genes, such as IL-1β, iNOS, MCP-1, IL-6 and CD11c but not tumor necrosis factor (TNF)-α. Notably, the effect of APS on inflammatory genes, except for TNF-α, was abrogated when AMPK activity was inhibited using a DN-AMPK plasmid. These results suggest that APS effectively ameliorates palmitate-induced pro-inflammatory responses through AMPK activity.
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1 

Greenfield JR and Campbell LV: Relationship between inflammation, insulin resistance and type 2 diabetes: ‘cause or effect’? Curr Diabetes Rev. 2:195–211. 2006.

2 

Permana PA, Menge C and Reaven PD: Macrophage-secreted factors induce adipocyte inflammation and insulin resistance. Biochem Biophys Res Commun. 341:507–514. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Bray GA, Lovejoy JC, Smith SR, et al: The influence of different fats and fatty acids on obesity, insulin resistance and inflammation. J Nutr. 132:2488–2491. 2002.PubMed/NCBI

4 

Samokhvalov V, Bilan PJ, Schertzer JD, Antonescu CN and Klip A: Palmitate- and lipopolysaccharide-activated macrophages evoke contrasting insulin responses in muscle cells. Am J Physiol Endocrinol Metab. 296:E37–E46. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Nguyen MT, Favelyukis S, Nguyen AK, et al: A subpopulation of macrophages infiltrates hypertrophic adipose tissue and is activated by free fatty acids via Toll-like receptors 2 and 4 and JNK-dependent pathways. J Biol Chem. 282:35279–35292. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Jeong HW, Hsu KC, Lee JW, et al: Berberine suppresses proinflammatory responses through AMPK activation in macrophages. Am J Physiol Endocrinol Metab. 296:E955–E964. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Denzler KL, Waters R, Jacobs BL, Rochon Y and Langland JO: Regulation of inflammatory gene expression in PBMCs by immunostimulatory botanicals. PLoS One. 5:e125612010. View Article : Google Scholar : PubMed/NCBI

8 

Chen W, Xia YP, Chen WJ, Yu MH, Li YM and Ye HY: Improvement of myocardial glycolipid metabolic disorder in diabetic hamster with Astragalus polysaccharide treatment. Mol Biol Rep. 39:7609–7615. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Liu M, Wu K, Mao X, Wu Y and Ouyang J: Astragalus polysaccharide improves insulin sensitivity in KKAy mice: regulation of PKB/GLUT4 signaling in skeletal muscle. J Ethnopharmacol. 127:32–37. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Chen W, Li Y and Yu M: Astragalus polysaccharides: an effective treatment for diabetes prevention in NOD mice. Exp Clin Endocrinol Diabetes. 116:468–474. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Li RJ, Qiu SD, Chen HX, Tian H and Wang HX: The immunotherapeutic effects of Astragalus polysaccharide in type 1 diabetic mice. Biol Pharm Bull. 30:470–476. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Zou F, Mao XQ, Wang N, Liu J and Ou-Yang JP: Astragalus polysaccharides alleviate glucose toxicity and restores glucose homeostasis in diabetic states via activation of AMPK. Acta Pharmacol Sin. 30:1607–1615. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Sag D, Carling D, Stout RD and Suttles J: Adenosine 5′-monophosphate-activated protein kinase promotes macrophage polarization to an anti-inflammatory functional phenotype. J Immunol. 181:8633–8641. 2008.

14 

Shon YH, Kim JH and Nam KS: Effect of Astragali radix extract on lipopolysaccharide-induced inflammation in human amnion. Biol Pharm Bull. 25:77–80. 2002. View Article : Google Scholar : PubMed/NCBI

15 

He X, Shu J, Xu L, Lu C and Lu A: Inhibitory Effect of Astragalus polysaccharides on lipopolysaccharide-induced TNF-α and IL-1β production in THP-1 cells. Molecules. 17:3155–3164. 2012.PubMed/NCBI

16 

Zhao LH, Ma ZX, Zhu J, Yu XH and Weng DP: Characterization of polysaccharide from Astragalus radix as the macrophage stimulator. Cell Immunol. 271:329–334. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Lee KY and Jeon YJ: Macrophage activation by polysaccharide isolated from Astragalus membranaceus. Int Immunopharmacol. 5:1225–1233. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Wang N, Zhang D, Mao X, Zou F, Jin H and Ouyang J: Astragalus polysaccharides decreased the expression of PTP1B through relieving ER stress induced activation of ATF6 in a rat model of type 2 diabetes. Mol Cell Endocrinol. 307:89–98. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Schmitz-Peiffer C, Craig DL and Biden TJ: Ceramide generation is sufficient to account for the inhibition of the insulin-stimulated PKB pathway in C2C12 skeletal muscle cells pretreated with palmitate. J Biol Chem. 274:24202–24210. 1999. View Article : Google Scholar

20 

Lefevre L, Gales A, Olagnier D, et al: PPARgamma ligands switched high fat diet-induced macrophage M2b polarization toward M2a thereby improving intestinal Candida elimination. PLoS One. 5:e128282010. View Article : Google Scholar : PubMed/NCBI

21 

Oh DY, Talukdar S, Bae EJ, et al: GPR120 is an omega-3 fatty acid receptor mediating potent anti-inflammatory and insulin-sensitizing effects. Cell. 142:687–698. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Hong EG, Ko HJ, Cho YR, et al: Interleukin-10 prevents diet-induced insulin resistance by attenuating macrophage and cytokine response in skeletal muscle. Diabetes. 58:2525–2535. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Gabay C, Lamacchia C and Palmer G: IL-1 pathways in inflammation and human diseases. Nat Rev Rheumatol. 6:232–241. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Speaker KJ and Fleshner M: Interleukin-1 beta: a potential link between stress and the development of visceral obesity. BMC Physiol. 12:82012. View Article : Google Scholar : PubMed/NCBI

25 

Schimmack G, Defronzo RA and Musi N: AMP-activated protein kinase: role in metabolism and therapeutic implications. Diabetes Obes Metab. 8:591–602. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Hattori Y, Suzuki K, Hattori S and Kasai K: Metformin inhibits cytokine-induced nuclear factor kappaB activation via AMP-activated protein kinase activation in vascular endothelial cells. Hypertension. 47:1183–1188. 2006. View Article : Google Scholar

27 

Yuan Y, Sun M and Li KS: Astragalus mongholicus polysaccharide inhibits lipopolysaccharide-induced production of TNF-alpha and interleukin-8. World J Gastroenterol. 15:3676–3680. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Jiang JB, Qiu JD, Yang LH, He JP, Smith GW and Li HQ: Therapeutic effects of astragalus polysaccharides on inflammation and synovial apoptosis in rats with adjuvant-induced arthritis. Int J Rheum Dis. 13:396–405. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lu J, Chen X, Zhang Y, Xu J, Zhang L, Li Z, Liu W, Ouyang J, Han S, He X, He X, et al: Astragalus polysaccharide induces anti-inflammatory effects dependent on AMPK activity in palmitate-treated RAW264.7 cells. Int J Mol Med 31: 1463-1470, 2013.
APA
Lu, J., Chen, X., Zhang, Y., Xu, J., Zhang, L., Li, Z. ... He, X. (2013). Astragalus polysaccharide induces anti-inflammatory effects dependent on AMPK activity in palmitate-treated RAW264.7 cells. International Journal of Molecular Medicine, 31, 1463-1470. https://doi.org/10.3892/ijmm.2013.1335
MLA
Lu, J., Chen, X., Zhang, Y., Xu, J., Zhang, L., Li, Z., Liu, W., Ouyang, J., Han, S., He, X."Astragalus polysaccharide induces anti-inflammatory effects dependent on AMPK activity in palmitate-treated RAW264.7 cells". International Journal of Molecular Medicine 31.6 (2013): 1463-1470.
Chicago
Lu, J., Chen, X., Zhang, Y., Xu, J., Zhang, L., Li, Z., Liu, W., Ouyang, J., Han, S., He, X."Astragalus polysaccharide induces anti-inflammatory effects dependent on AMPK activity in palmitate-treated RAW264.7 cells". International Journal of Molecular Medicine 31, no. 6 (2013): 1463-1470. https://doi.org/10.3892/ijmm.2013.1335
Copy and paste a formatted citation
x
Spandidos Publications style
Lu J, Chen X, Zhang Y, Xu J, Zhang L, Li Z, Liu W, Ouyang J, Han S, He X, He X, et al: Astragalus polysaccharide induces anti-inflammatory effects dependent on AMPK activity in palmitate-treated RAW264.7 cells. Int J Mol Med 31: 1463-1470, 2013.
APA
Lu, J., Chen, X., Zhang, Y., Xu, J., Zhang, L., Li, Z. ... He, X. (2013). Astragalus polysaccharide induces anti-inflammatory effects dependent on AMPK activity in palmitate-treated RAW264.7 cells. International Journal of Molecular Medicine, 31, 1463-1470. https://doi.org/10.3892/ijmm.2013.1335
MLA
Lu, J., Chen, X., Zhang, Y., Xu, J., Zhang, L., Li, Z., Liu, W., Ouyang, J., Han, S., He, X."Astragalus polysaccharide induces anti-inflammatory effects dependent on AMPK activity in palmitate-treated RAW264.7 cells". International Journal of Molecular Medicine 31.6 (2013): 1463-1470.
Chicago
Lu, J., Chen, X., Zhang, Y., Xu, J., Zhang, L., Li, Z., Liu, W., Ouyang, J., Han, S., He, X."Astragalus polysaccharide induces anti-inflammatory effects dependent on AMPK activity in palmitate-treated RAW264.7 cells". International Journal of Molecular Medicine 31, no. 6 (2013): 1463-1470. https://doi.org/10.3892/ijmm.2013.1335
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