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Article

Tougu Xiaotong capsule inhibits the tidemark replication and cartilage degradation of papain-induced osteoarthritis by the regulation of chondrocyte autophagy

  • Authors:
    • Xihai Li
    • Wenna Lang
    • Hongzhi Ye
    • Fangrong Yu
    • Huiting Li
    • Jiashou Chen
    • Liangliang Cai
    • Wenlie Chen
    • Ruhui Lin
    • Yunmei Huang
    • Xianxiang Liu
  • View Affiliations / Copyright

    Affiliations: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, P.R. China, Research Base of Traditional Chinese Medicine Syndrome, Fujian University of Traditional Chinese Medicine, Fuzhou, P.R. China, Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China
  • Pages: 1349-1356
    |
    Published online on: April 9, 2013
       https://doi.org/10.3892/ijmm.2013.1341
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Abstract

The tidemark is located between calcified and non-calcified cartilage matrices. Tidemark replication plays an important role in the pathogenesis of osteoarthrosis (OA). Autophagy, or cellular self-digestion, is an essential cellular homeostasis mechanism that was found to be deficient in osteoarthritic cartilage. This study evaluated the effects of Tougu Xiaotong capsule (TXC) on the tidemark replication and cartilage degradation, and also investigated LC3 I/II, which executes autophagy, the potential role of ULK1, an inducer of autophagy, and Beclin1, a regulator of autophagy, in the development of a papain-induced OA in rat knee joints. Using a papain-injected knee rat model, standard histological methods were used to validate our model as well as treatment with TXC or glucosamine (GlcN). After 12 weeks of treatment, the changes of cartilage structure were observed by digital radiography (DR), optical microscopy, scanning electron microscopy and transmission electron microscopy, and the LC3 I/II, ULK1 and Beclin1 levels were measured by western blotting. Cartilage degradation was evaluated by the Mankin score on paraffin-embedded sections stained with Safranin O-fast green. TXC was found to improve the arrangement of subchondral bone collagen fibers and calcium phosphate crystals, inhibit the tidemark replication and delay the cartilage degradation in the papain-induced OA. Our results also showed that LC3 I/II, ULK1 and Beclin1 levels in both the TXC+OA and GlcN+OA groups were significantly increased compared to those in the OA group. The results indicate that TXC could inhibit the tidemark replication and cartilage degradation by the regulation of chondrocyte autophagy.
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1 

Li X, Ye H, Yu F, et al: Millimeter wave treatment promotes chondrocyte proliferation via G1/S cell cycle transition. Int J Mol Med. 29:823–831. 2012.PubMed/NCBI

2 

Wu MX, Li XH, Lin MN, et al: Clinical study on the treatment of knee osteoarthritis of shensui insufficiency syndrome type by electroacupuncture. Chin J Integr Med. 16:291–297. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Galois L, Etienne S, Grossin L, et al: Dose-response relationship for exercise on severity of experimental osteoarthritis in rats: a pilot study. Osteoarthritis Cartilage. 12:779–786. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Panicker S, Borgia J, Fhied C, Mikecz K and Oegema TR: Oral glucosamine modulates the response of the liver and lymphocytes of the mesenteric lymph nodes in a papain-induced model of joint damage and repair. Osteoarthritis Cartilage. 17:1014–1021. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Eswaramoorthy R, Chang CC, Wu SC, Wang GJ, Chang JK and Ho ML: Sustained release of PTH(1-34) from PLGA microspheres suppresses osteoarthritis progression in rats. Acta Biomater. 8:2254–2262. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Bonde HV, Talman ML and Kofoed H: The area of the tidemark in osteoarthritis - a three-dimensional stereological study in 21 patients. APMIS. 113:349–352. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Lyons TJ, Stoddart RW, McClure SF and McClure J: The tidemark of the chondro-osseous junction of the normal human knee joint. J Mol Histol. 36:207–215. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Dequeker J, Mokassa L, Aerssens J and Boonen S: Bone density and local growth factors in generalized osteoarthritis. Microsc Res Tech. 37:358–371. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Del Carlo M Jr and Loeser RF: Cell death in osteoarthritis. Curr Rheumatol Rep. 10:37–42. 2008.PubMed/NCBI

10 

Almonte-Becerril M, Navarro-Garcia F, Gonzalez-Robles A, Vega-Lopez MA, Lavalle C and Kouri JB: Cell death of chondrocytes is a combination between apoptosis and autophagy during the pathogenesis of Osteoarthritis within an experimental model. Apoptosis. 15:631–638. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Kim HA, Lee YJ, Seong SC, Choe KW and Song YW: Apoptotic chondrocyte death in human osteoarthritis. J Rheumatol. 27:455–462. 2000.

12 

Caramés B, Taniguchi N, Otsuki S, Blanco FJ and Lotz M: Autophagy is a protective mechanism in normal cartilage, and its aging-related loss is linked with cell death and osteoarthritis. Arthritis Rheum. 62:791–801. 2010.PubMed/NCBI

13 

Lotz MK and Caramés B: Autophagy and cartilage homeostasis mechanisms in joint health, aging and OA. Nat Rev Rheumatol. 7:579–587. 2011.PubMed/NCBI

14 

Li XH, Wu MX, Ye HZ, et al: Experimental study on the suppression of sodium nitroprussiate-induced chondrocyte apoptosis by Tougu Xiaotong Capsule-containing serum. Chin J Integr Med. 17:436–443. 2011. View Article : Google Scholar

15 

Kellgren JH and Lawrence JS: Radiological assessment of rheumatoid arthritis. Ann Rheheum Dis. 16:485–493. 1957. View Article : Google Scholar : PubMed/NCBI

16 

Mankin HJ, Dorfman H, Lippiello L and Zarins A: Biochemical and metabolic abnormalities in articular cartilage from osteoarthritic human hips. II. Correlation of morphology with biochemical and metabolic data. J Bone Joint Surg Am. 53:523–537. 1971.PubMed/NCBI

17 

Klop C, de Vries F, Lalmohamed A, et al: COX-2-selective NSAIDs and risk of hip or knee replacements: a population-based case-control study. Calcif Tissue Int. 91:387–394. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Zoeger N, Roschger P, Hofstaetter JG, et al: Lead accumulation in tidemark of articular cartilage. Osteoarthritis Cartilage. 14:906–913. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Gannon FH and Sokoloff L: Histomorphometry of the aging human patella: histologic criteria and controls. Osteoarthritis Cartilage. 7:173–181. 1999. View Article : Google Scholar : PubMed/NCBI

20 

Meirer F, Pemmer B, Pepponi G, et al: Assessment of chemical species of lead accumulated in tidemarks of human articular cartilage by X-ray absorption near-edge structure analysis. J Synchrotron Radiat. 18:238–244. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Otterness IG, Chang M, Burkhardt JE, Sweeney FJ and Milici AJ: Histology and tissue chemistry of tidemark separation in hamsters. Vet Pathol. 36:138–145. 1999. View Article : Google Scholar : PubMed/NCBI

22 

Pan J, Wang B, Li W, et al: Elevated cross-talk between subchondral bone and cartilage in osteoarthritic joints. Bone. 51:212–217. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Lacourt M, Gao C, Li A, et al: Relationship between cartilage and subchondral bone lesions in repetitive impact trauma-induced equine osteoarthritis. Osteoarthritis Cartilage. 20:572–583. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Bobinac D, Spanjol J, Zoricic S and Maric I: Changes in articular cartilage and subchondral bone histomorphometry in osteoarthritic knee joints in humans. Bone. 32:284–290. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Burr DB: Increased biological activity of subchondral mineralized tissues underlies the progressive deterioration of articular cartilage in osteoarthritis. J Rheumatol. 32:1156–1158. 2005.PubMed/NCBI

26 

Botter SM, Glasson SS, Hopkins B, et al: ADAMTS5−/− mice have less subchondral bone changes after induction of osteo-arthritis through surgical instability: implications for a link between cartilage and subchondral bone changes. Osteoarthritis Cartilage. 17:636–645. 2009.

27 

Day JS, Ding M, van der Linden JC, Hvid I, Sumner DR and Weinans H: A decreased subchondral trabecular bone tissue elastic modulus is associated with pre-arthritic cartilage damage. J Orthop Res. 19:914–918. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Bennell KL, Creaby MW, Wrigley TV and Hunter DJ: Tibial subchondral trabecular volumetric bone density in medial knee joint osteoarthritis using peripheral quantitative computed tomography technology. Arthritis Rheum. 58:2776–2785. 2008.

29 

Aula AS, Töyräs J, Tiitu V and Jurvelin JS: Simultaneous ultrasound measurement of articular cartilage and subchondral bone. Osteoarthritis Cartilage. 18:1570–1576. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Mizushima N, Levine B, Cuervo AM and Klionsky DJ: Auto-phagy fights disease through cellular self-digestion. Nature. 451:1069–1075. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Srinivas V, Bohensky J, Zahm AM and Shapiro IM: Autophagy in mineralizing tissues: microenvironmental perspectives. Cell Cycle. 8:391–393. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Cajee UF, Hull R and Ntwasa M: Modification by ubiquitin-like proteins: significance in apoptosis and autophagy pathways. Int J Mol Sci. 13:11804–11831. 2012. View Article : Google Scholar : PubMed/NCBI

33 

Chan EY, Kir S and Tooze SA: siRNA screening of the kinome identifies ULK1 as a multidomain modulator of autophagy. J Biol Chem. 282:25464–25474. 2007. View Article : Google Scholar : PubMed/NCBI

34 

Furuya N, Yu J, Byfield M, Pattingre S and Levine B: The evolutionarily conserved domain of Beclin 1 is required for Vps34 binding, autophagy and tumor suppressor function. Autophagy. 1:46–52. 2005. View Article : Google Scholar : PubMed/NCBI

35 

Abounit K, Scarabelli TM and McCauley RB: Autophagy in mammalian cells. World J Biol Chem. 3:1–6. 2012.

36 

Ohsumi Y and Mizushima N: Two ubiquitin-like conjugation systems essential for autophagy. Semin Cell Dev Biol. 15:231–236. 2004. View Article : Google Scholar : PubMed/NCBI

37 

Kabeya Y, Mizushima N, Ueno T, et al: LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J. 19:5720–5728. 2000. View Article : Google Scholar : PubMed/NCBI

38 

Settembre C, Arteaga-Solis E, McKee MD, et al: Proteoglycan desulfation determines the efficiency of chondrocyte autophagy and the extent of FGF signaling during endochondral ossification. Genes Dev. 22:2645–2650. 2008. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Li X, Lang W, Ye H, Yu F, Li H, Chen J, Cai L, Chen W, Lin R, Huang Y, Huang Y, et al: Tougu Xiaotong capsule inhibits the tidemark replication and cartilage degradation of papain-induced osteoarthritis by the regulation of chondrocyte autophagy. Int J Mol Med 31: 1349-1356, 2013.
APA
Li, X., Lang, W., Ye, H., Yu, F., Li, H., Chen, J. ... Liu, X. (2013). Tougu Xiaotong capsule inhibits the tidemark replication and cartilage degradation of papain-induced osteoarthritis by the regulation of chondrocyte autophagy. International Journal of Molecular Medicine, 31, 1349-1356. https://doi.org/10.3892/ijmm.2013.1341
MLA
Li, X., Lang, W., Ye, H., Yu, F., Li, H., Chen, J., Cai, L., Chen, W., Lin, R., Huang, Y., Liu, X."Tougu Xiaotong capsule inhibits the tidemark replication and cartilage degradation of papain-induced osteoarthritis by the regulation of chondrocyte autophagy". International Journal of Molecular Medicine 31.6 (2013): 1349-1356.
Chicago
Li, X., Lang, W., Ye, H., Yu, F., Li, H., Chen, J., Cai, L., Chen, W., Lin, R., Huang, Y., Liu, X."Tougu Xiaotong capsule inhibits the tidemark replication and cartilage degradation of papain-induced osteoarthritis by the regulation of chondrocyte autophagy". International Journal of Molecular Medicine 31, no. 6 (2013): 1349-1356. https://doi.org/10.3892/ijmm.2013.1341
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Lang W, Ye H, Yu F, Li H, Chen J, Cai L, Chen W, Lin R, Huang Y, Huang Y, et al: Tougu Xiaotong capsule inhibits the tidemark replication and cartilage degradation of papain-induced osteoarthritis by the regulation of chondrocyte autophagy. Int J Mol Med 31: 1349-1356, 2013.
APA
Li, X., Lang, W., Ye, H., Yu, F., Li, H., Chen, J. ... Liu, X. (2013). Tougu Xiaotong capsule inhibits the tidemark replication and cartilage degradation of papain-induced osteoarthritis by the regulation of chondrocyte autophagy. International Journal of Molecular Medicine, 31, 1349-1356. https://doi.org/10.3892/ijmm.2013.1341
MLA
Li, X., Lang, W., Ye, H., Yu, F., Li, H., Chen, J., Cai, L., Chen, W., Lin, R., Huang, Y., Liu, X."Tougu Xiaotong capsule inhibits the tidemark replication and cartilage degradation of papain-induced osteoarthritis by the regulation of chondrocyte autophagy". International Journal of Molecular Medicine 31.6 (2013): 1349-1356.
Chicago
Li, X., Lang, W., Ye, H., Yu, F., Li, H., Chen, J., Cai, L., Chen, W., Lin, R., Huang, Y., Liu, X."Tougu Xiaotong capsule inhibits the tidemark replication and cartilage degradation of papain-induced osteoarthritis by the regulation of chondrocyte autophagy". International Journal of Molecular Medicine 31, no. 6 (2013): 1349-1356. https://doi.org/10.3892/ijmm.2013.1341
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