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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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June 2013 Volume 31 Issue 6

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

Upregulation of microRNA-155 promotes the migration and invasion of colorectal cancer cells through the regulation of claudin-1 expression

  • Authors:
    • Guang-Jun Zhang
    • Hua-Xu Xiao
    • Hong-Peng Tian
    • Zuo-Liang Liu
    • Shu-Sen Xia
    • Tong Zhou
  • View Affiliations / Copyright

    Affiliations: First Department of General Surgery, Institute of Hepatobiliary, Pancreatic and Intestinal Disease, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China
  • Pages: 1375-1380
    |
    Published online on: April 15, 2013
       https://doi.org/10.3892/ijmm.2013.1348
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Abstract

Human microRNA-155 (miR-155) has been demonstrated to regulate a variety of cellular functions, including epithelial-to-mesenchymal transition (EMT) by targeting multiple messenger RNAs (mRNAs). However, its role in colorectal cancer (CRC) remains unelucidated. Therefore, the aim of the present study was to investigate the effects of miR-155 on CRC cells. The expression level of miR-155 was quantified by quantitative real-time reverse transcriptase-PCR (qRT-PCR) in primary CRC tissues and normal adjacent mucosa. MTT, migration and invasion assays were used to examine the proliferation, migration and invasion of SW480 cells transfected with miR‑155. The expression of miR-155 was significantly upregulated in the CRC tissues and the high expression of miR-155 correlated with an advanced clinical stage, lymph node and distant metastases. The ectopic expression of miR-155 enhanced the migration and invasive ability of the SW480 cells and altered their morphological appearance; however, cell proliferation was not affected. E-cadherin expression levels decreased, while ZEB1 expression levels increased in the SW480 cells overexpressing miR-155. Furthermore, the overexpression of miR-155 upregulated claudin-1 expression. Thus, our data suggest that miR-155 plays an important role in promoting CRC cell migration and invasion, at least in part through the regulation of claudin-1 expression and controlling metastasis in CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang G, Xiao H, Tian H, Liu Z, Xia S and Zhou T: Upregulation of microRNA-155 promotes the migration and invasion of colorectal cancer cells through the regulation of claudin-1 expression. Int J Mol Med 31: 1375-1380, 2013.
APA
Zhang, G., Xiao, H., Tian, H., Liu, Z., Xia, S., & Zhou, T. (2013). Upregulation of microRNA-155 promotes the migration and invasion of colorectal cancer cells through the regulation of claudin-1 expression. International Journal of Molecular Medicine, 31, 1375-1380. https://doi.org/10.3892/ijmm.2013.1348
MLA
Zhang, G., Xiao, H., Tian, H., Liu, Z., Xia, S., Zhou, T."Upregulation of microRNA-155 promotes the migration and invasion of colorectal cancer cells through the regulation of claudin-1 expression". International Journal of Molecular Medicine 31.6 (2013): 1375-1380.
Chicago
Zhang, G., Xiao, H., Tian, H., Liu, Z., Xia, S., Zhou, T."Upregulation of microRNA-155 promotes the migration and invasion of colorectal cancer cells through the regulation of claudin-1 expression". International Journal of Molecular Medicine 31, no. 6 (2013): 1375-1380. https://doi.org/10.3892/ijmm.2013.1348
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang G, Xiao H, Tian H, Liu Z, Xia S and Zhou T: Upregulation of microRNA-155 promotes the migration and invasion of colorectal cancer cells through the regulation of claudin-1 expression. Int J Mol Med 31: 1375-1380, 2013.
APA
Zhang, G., Xiao, H., Tian, H., Liu, Z., Xia, S., & Zhou, T. (2013). Upregulation of microRNA-155 promotes the migration and invasion of colorectal cancer cells through the regulation of claudin-1 expression. International Journal of Molecular Medicine, 31, 1375-1380. https://doi.org/10.3892/ijmm.2013.1348
MLA
Zhang, G., Xiao, H., Tian, H., Liu, Z., Xia, S., Zhou, T."Upregulation of microRNA-155 promotes the migration and invasion of colorectal cancer cells through the regulation of claudin-1 expression". International Journal of Molecular Medicine 31.6 (2013): 1375-1380.
Chicago
Zhang, G., Xiao, H., Tian, H., Liu, Z., Xia, S., Zhou, T."Upregulation of microRNA-155 promotes the migration and invasion of colorectal cancer cells through the regulation of claudin-1 expression". International Journal of Molecular Medicine 31, no. 6 (2013): 1375-1380. https://doi.org/10.3892/ijmm.2013.1348
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