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Article

Induction of thymic stromal lymphopoietin expression in 16-HBE human bronchial epithelial cells by 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3

  • Authors:
    • Dan Zhang
    • Chenghua Peng
    • Haijin Zhao
    • Yang Xia
    • Dandan Zhang
    • Hangming Dong
    • Jiafu Song
    • Liqin Zhou
    • Shaoxi Cai
    • Fei Zou
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory and Critical Care Medicine, Chronic Airways Diseases Laboratory, Nanfang Hospital, Guangzhou, Guangdong 510515, P.R. China, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
  • Pages: 203-210
    |
    Published online on: April 18, 2013
       https://doi.org/10.3892/ijmm.2013.1353
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Abstract

Vitamin D exerts profound effects on airway epithelial cells. Thymic stromal lymphopoietin (TSLP) derived from airway epithelial cells plays a role in the innate and antigen‑specific adaptive immune responses. However, the effect of vitamin D on TSLP expression in airway epithelial cells is unclear. In this study, 16-HBE human bronchial epithelial (HBE) cells were cultured with various concentrations of 25-hydroxyvitamin D3 (25 D3) and 1,25-dihydroxyvitamin D3 (1,25 D3). The expression of TSLP in the 16-HBE human bronchial epithelial cell line was analyzed by PCR and enzyme-linked immunosorbent assay (ELISA). We found that the 16-HBE cells converted inactive 25 D3 to active 1,25 D3 and that TSLP mRNA and protein expression levels were significantly increased, peaking at 2 or 12 h in the cells exposed to 500 nM 25 D3 and 50 nM 1,25 D3 respectively. Since vitamin D3 upregulated protein 1 (VDUP1) plays a multifunctional role in a variety of cellular responses, we hypothesized that VDUP1 is involved in the induction of TSLP production by 25 D3. The results showed that the mRNA and protein levels of VDUP1 were significantly upregulated by vitamin D. Furthermore, the silencing of VDUP1 by small interfering RNA (siRNA) significantly inhibited the 25 D3- and 1,25 D3-mediated induction of TSLP expression. To characterize the metabolic properties of vitamin D in airway epithelial biology, we used the chemical inhibitor of 1α-hydroxylase, itraconazole. The results revealed that itraconazole (10-6 M) reduced the 25 D3- but not the 1,25 D3-induced TSLP expression in 16-HBE cells. Based on these data, it can be concluded that vitamin D increases TSLP expression in 16-HBE cells through the VDUP1 pathway, which suggests a novel mechanism by which vitamin D alters immune function in the lungs.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang D, Peng C, Zhao H, Xia Y, Zhang D, Dong H, Song J, Zhou L, Cai S, Zou F, Zou F, et al: Induction of thymic stromal lymphopoietin expression in 16-HBE human bronchial epithelial cells by 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3. Int J Mol Med 32: 203-210, 2013.
APA
Zhang, D., Peng, C., Zhao, H., Xia, Y., Zhang, D., Dong, H. ... Zou, F. (2013). Induction of thymic stromal lymphopoietin expression in 16-HBE human bronchial epithelial cells by 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3. International Journal of Molecular Medicine, 32, 203-210. https://doi.org/10.3892/ijmm.2013.1353
MLA
Zhang, D., Peng, C., Zhao, H., Xia, Y., Zhang, D., Dong, H., Song, J., Zhou, L., Cai, S., Zou, F."Induction of thymic stromal lymphopoietin expression in 16-HBE human bronchial epithelial cells by 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3". International Journal of Molecular Medicine 32.1 (2013): 203-210.
Chicago
Zhang, D., Peng, C., Zhao, H., Xia, Y., Zhang, D., Dong, H., Song, J., Zhou, L., Cai, S., Zou, F."Induction of thymic stromal lymphopoietin expression in 16-HBE human bronchial epithelial cells by 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3". International Journal of Molecular Medicine 32, no. 1 (2013): 203-210. https://doi.org/10.3892/ijmm.2013.1353
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang D, Peng C, Zhao H, Xia Y, Zhang D, Dong H, Song J, Zhou L, Cai S, Zou F, Zou F, et al: Induction of thymic stromal lymphopoietin expression in 16-HBE human bronchial epithelial cells by 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3. Int J Mol Med 32: 203-210, 2013.
APA
Zhang, D., Peng, C., Zhao, H., Xia, Y., Zhang, D., Dong, H. ... Zou, F. (2013). Induction of thymic stromal lymphopoietin expression in 16-HBE human bronchial epithelial cells by 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3. International Journal of Molecular Medicine, 32, 203-210. https://doi.org/10.3892/ijmm.2013.1353
MLA
Zhang, D., Peng, C., Zhao, H., Xia, Y., Zhang, D., Dong, H., Song, J., Zhou, L., Cai, S., Zou, F."Induction of thymic stromal lymphopoietin expression in 16-HBE human bronchial epithelial cells by 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3". International Journal of Molecular Medicine 32.1 (2013): 203-210.
Chicago
Zhang, D., Peng, C., Zhao, H., Xia, Y., Zhang, D., Dong, H., Song, J., Zhou, L., Cai, S., Zou, F."Induction of thymic stromal lymphopoietin expression in 16-HBE human bronchial epithelial cells by 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3". International Journal of Molecular Medicine 32, no. 1 (2013): 203-210. https://doi.org/10.3892/ijmm.2013.1353
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