Utilization of microRNAs with decreased expression levels in response to X-ray irradiation for fine-tuning radiation-controlled gene regulation

  • Authors:
    • Akihiro Morii
    • Ryohei Ogawa
    • Akihiko Watanabe
    • Zeng-Guo Cui
    • Ichiro Takasaki
    • Nobutaka Doi
    • Takashi Kondo
    • Hideki Fuse
  • View Affiliations

  • Published online on: April 23, 2013     https://doi.org/10.3892/ijmm.2013.1360
  • Pages: 9-16
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Abstract

We previously developed a promoter that was responsive to radiation by randomly combining cis-elements of transcription factors that are activated in response to radiation in prostate cancer cells. The promoter enhanced the expression of the luciferase gene linked downstream by more than 10-fold 12 h after X-ray irradiation at 10 Gy. However, without radiation, it still significantly drove its expression. To suppress expression while retaining its enhancement in response to radiation, we focused our attention on microRNAs (miRNAs). miRNAs are a group of non-coding RNAs approximately 22 nucleotides long that control gene expression by binding to a target sequence residing on the 3'-untranslated region (3'UTR) of a target gene. We identified 8 miRNAs that were downregulated in response to X-ray irradiation, and inserted artificial target sequences composed of randomly combined complementary sequences into 3 representative miRNAs into the 3'UTR of the luciferase gene. The target sequences suppressed the expression, and released the expression, after X-ray irradiation, as expected. When we combined an artificial target sequence with the radiation-responsive promoter, it resulted in a clear-cut gene regulation of expression that was greater than that induced by the promoter alone.
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July 2013
Volume 32 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Morii A, Ogawa R, Watanabe A, Cui Z, Takasaki I, Doi N, Kondo T and Fuse H: Utilization of microRNAs with decreased expression levels in response to X-ray irradiation for fine-tuning radiation-controlled gene regulation. Int J Mol Med 32: 9-16, 2013
APA
Morii, A., Ogawa, R., Watanabe, A., Cui, Z., Takasaki, I., Doi, N. ... Fuse, H. (2013). Utilization of microRNAs with decreased expression levels in response to X-ray irradiation for fine-tuning radiation-controlled gene regulation. International Journal of Molecular Medicine, 32, 9-16. https://doi.org/10.3892/ijmm.2013.1360
MLA
Morii, A., Ogawa, R., Watanabe, A., Cui, Z., Takasaki, I., Doi, N., Kondo, T., Fuse, H."Utilization of microRNAs with decreased expression levels in response to X-ray irradiation for fine-tuning radiation-controlled gene regulation". International Journal of Molecular Medicine 32.1 (2013): 9-16.
Chicago
Morii, A., Ogawa, R., Watanabe, A., Cui, Z., Takasaki, I., Doi, N., Kondo, T., Fuse, H."Utilization of microRNAs with decreased expression levels in response to X-ray irradiation for fine-tuning radiation-controlled gene regulation". International Journal of Molecular Medicine 32, no. 1 (2013): 9-16. https://doi.org/10.3892/ijmm.2013.1360