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International Journal of Molecular Medicine
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Article

Hexane extract of aged black garlic reduces cell proliferation and attenuates the expression of ICAM-1 and VCAM‑1 in TNF-α-activated human endometrial stromal cells

  • Authors:
    • Ki-Hyung Kim
    • Jin Kyeong Park
    • Young-Whan Choi
    • Youn-Han Kim
    • Eun Na Lee
    • Ja-Rang Lee
    • Heui-Soo Kim
    • Sun-Yong Baek
    • Bong-Seon Kim
    • Kyu-Sup Lee
    • Sik Yoon
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Pusan National University School of Medicine, Busan 602-739, Republic of Korea, Department of Anatomy, Pusan National University School of Medicine, Yangsan 626-870, Republic of Korea, Department of Horticultural Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang 627-706, Republic of Korea, Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea
  • Pages: 67-78
    |
    Published online on: April 25, 2013
       https://doi.org/10.3892/ijmm.2013.1362
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Abstract

Increasing evidence indicates the potentially crucial roles of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in the pathological process underlying endometriosis. The present study aimed to investigate the effects of a hexane extract of aged black garlic (HEABG) on the proliferation and expression of ICAM-1 and VCAM-1 in tumor necrosis factor-α (TNF-α)-activated human endometrial stromal cells (HESCs) isolated from patients with endometriosis. HESCs were isolated from endometriotic tissues obtained from women with advanced endometriosis who underwent laparoscopic surgery for ovarian endometrioma (n=18). Cell proliferation and cell cycle analysis were assessed by WST-1 assay and flow cytometry, respectively. The expression of ICAM-1 and VCAM-1 was measured by flow cytometry, immunofluorescence staining, immunoblotting and quantitative reverse transcriptase-PCR. The secretion of interleukin-6 (IL-6) was determined by enzyme-linked immunosorbent assay (ELISA). The activation of nuclear factor-κB (NF-κB) and activator protein-1 (AP-1) was detected by electrophoretic mobility shift assay (EMSA) and the activation of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and p38 MAPK was analyzed by immunoblotting. Cell proliferation and cell cycle progression were significantly suppressed by HEABG in the TNF-α-induced HESCs through the inhibition of the ERK and JNK signaling pathways. Remarkably, the treatment of the HESCs with HEABG potently suppressed the TNF-α-induced ICAM-1 and VCAM-1 transcript and protein expression by inhibiting the activation of NF-κB and AP-1 transcription factors. Our results suggest that HEABG may be effective in the prevention and treatment of endometriosis in humans.
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Copy and paste a formatted citation
Spandidos Publications style
Kim K, Park JK, Choi Y, Kim Y, Lee EN, Lee J, Kim H, Baek S, Kim B, Lee K, Lee K, et al: Hexane extract of aged black garlic reduces cell proliferation and attenuates the expression of ICAM-1 and VCAM‑1 in TNF-α-activated human endometrial stromal cells. Int J Mol Med 32: 67-78, 2013.
APA
Kim, K., Park, J.K., Choi, Y., Kim, Y., Lee, E.N., Lee, J. ... Yoon, S. (2013). Hexane extract of aged black garlic reduces cell proliferation and attenuates the expression of ICAM-1 and VCAM‑1 in TNF-α-activated human endometrial stromal cells. International Journal of Molecular Medicine, 32, 67-78. https://doi.org/10.3892/ijmm.2013.1362
MLA
Kim, K., Park, J. K., Choi, Y., Kim, Y., Lee, E. N., Lee, J., Kim, H., Baek, S., Kim, B., Lee, K., Yoon, S."Hexane extract of aged black garlic reduces cell proliferation and attenuates the expression of ICAM-1 and VCAM‑1 in TNF-α-activated human endometrial stromal cells". International Journal of Molecular Medicine 32.1 (2013): 67-78.
Chicago
Kim, K., Park, J. K., Choi, Y., Kim, Y., Lee, E. N., Lee, J., Kim, H., Baek, S., Kim, B., Lee, K., Yoon, S."Hexane extract of aged black garlic reduces cell proliferation and attenuates the expression of ICAM-1 and VCAM‑1 in TNF-α-activated human endometrial stromal cells". International Journal of Molecular Medicine 32, no. 1 (2013): 67-78. https://doi.org/10.3892/ijmm.2013.1362
Copy and paste a formatted citation
x
Spandidos Publications style
Kim K, Park JK, Choi Y, Kim Y, Lee EN, Lee J, Kim H, Baek S, Kim B, Lee K, Lee K, et al: Hexane extract of aged black garlic reduces cell proliferation and attenuates the expression of ICAM-1 and VCAM‑1 in TNF-α-activated human endometrial stromal cells. Int J Mol Med 32: 67-78, 2013.
APA
Kim, K., Park, J.K., Choi, Y., Kim, Y., Lee, E.N., Lee, J. ... Yoon, S. (2013). Hexane extract of aged black garlic reduces cell proliferation and attenuates the expression of ICAM-1 and VCAM‑1 in TNF-α-activated human endometrial stromal cells. International Journal of Molecular Medicine, 32, 67-78. https://doi.org/10.3892/ijmm.2013.1362
MLA
Kim, K., Park, J. K., Choi, Y., Kim, Y., Lee, E. N., Lee, J., Kim, H., Baek, S., Kim, B., Lee, K., Yoon, S."Hexane extract of aged black garlic reduces cell proliferation and attenuates the expression of ICAM-1 and VCAM‑1 in TNF-α-activated human endometrial stromal cells". International Journal of Molecular Medicine 32.1 (2013): 67-78.
Chicago
Kim, K., Park, J. K., Choi, Y., Kim, Y., Lee, E. N., Lee, J., Kim, H., Baek, S., Kim, B., Lee, K., Yoon, S."Hexane extract of aged black garlic reduces cell proliferation and attenuates the expression of ICAM-1 and VCAM‑1 in TNF-α-activated human endometrial stromal cells". International Journal of Molecular Medicine 32, no. 1 (2013): 67-78. https://doi.org/10.3892/ijmm.2013.1362
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