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Article

Crude extract of Ceriporia lacerata has a protective effect on dexamethasone-induced cytotoxicity in INS-1 cells via the modulation of PI3K/PKB activity

  • Authors:
    • Ji-Hye Kim
    • Yu-Kyoung Park
    • Ji-Eun Kim
    • Sam-Pin Lee
    • Byoung-Cheon Kim
    • Byeong-Churl Jang
  • View Affiliations / Copyright

    Affiliations: Department of Medical Genetic Engineering, Keimyung University School of Medicine, Dalseo-gu, Daegu 704-701, Republic of Korea, Worldbiotech Co, Ltd., Sangju, Gyeongbuk 742-912, Republic of Korea, Department of Food Science and Technology, Keimyung University, Dalseo-gu, Daegu 704-701, Republic of Korea
  • Pages: 179-186
    |
    Published online on: April 26, 2013
       https://doi.org/10.3892/ijmm.2013.1364
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Abstract

Excessive and/or long-term glucocorticoid therapy reduces β-cell mass and induces hyperglycemia, which contribute to the development of steroid‑induced diabetes. Ceriporia (C.) lacerata is one of the white‑rot fungi and has been used in bioremediations, such as lignocellulose degradation, in nature. The pharmacologic effect of C. lacerata on steroid-induced β-cell toxicity is not known. In this study, we evaluated the effect of a crude extract from a submerged cultivation of C. lacerata on the survival and apoptosis of INS-1 rat insulin-secreting cells exposed to dexamethasone (Dex), a synthetic diabetogenic glucocorticoid. Treatment with the C. lacerata crude extract (CLCE) largely blocked the Dex-induced reduction in survival and apoptosis of INS-1 cells. Moreover, CLCE treatment inhibited Dex-induced protein kinase B (PKB) dephosphorylation without affecting Dex-induced extracellular signal-regulated protein kinase-1/2 dephosphorylation and MKP-1 upregulation. Importantly, the protective effect of CLCE on Dex-induced cytotoxicity in INS-1 cells was attenuated by LY294002, an inhibitor of PI3K/PKB. CLCE treatment, however, did not protect the INS-1 cells from the cytotoxic effects triggered by other insults, such as interleukin-1β (an inflammatory cytokine), streptozotocin (a diabetogenic drug), thapsigargin (a calcium mobilizing agent), and tunicamycin (an ER stress inducer). Collectively, these findings demonstrate for the first time the ability of CLCE to specifically protect INS-1 cells from Dex-induced cytotoxicity through the modulation of the PI3K/PKB pathway. It is suggested that CLCE may be applied for the prevention and/or treatment of steroid diabetes in which reduction of β-cell survival and induction of β-cell apoptosis play pathogenic roles.
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Copy and paste a formatted citation
Spandidos Publications style
Kim J, Park Y, Kim J, Lee S, Kim B and Jang B: Crude extract of Ceriporia lacerata has a protective effect on dexamethasone-induced cytotoxicity in INS-1 cells via the modulation of PI3K/PKB activity. Int J Mol Med 32: 179-186, 2013.
APA
Kim, J., Park, Y., Kim, J., Lee, S., Kim, B., & Jang, B. (2013). Crude extract of Ceriporia lacerata has a protective effect on dexamethasone-induced cytotoxicity in INS-1 cells via the modulation of PI3K/PKB activity. International Journal of Molecular Medicine, 32, 179-186. https://doi.org/10.3892/ijmm.2013.1364
MLA
Kim, J., Park, Y., Kim, J., Lee, S., Kim, B., Jang, B."Crude extract of Ceriporia lacerata has a protective effect on dexamethasone-induced cytotoxicity in INS-1 cells via the modulation of PI3K/PKB activity". International Journal of Molecular Medicine 32.1 (2013): 179-186.
Chicago
Kim, J., Park, Y., Kim, J., Lee, S., Kim, B., Jang, B."Crude extract of Ceriporia lacerata has a protective effect on dexamethasone-induced cytotoxicity in INS-1 cells via the modulation of PI3K/PKB activity". International Journal of Molecular Medicine 32, no. 1 (2013): 179-186. https://doi.org/10.3892/ijmm.2013.1364
Copy and paste a formatted citation
x
Spandidos Publications style
Kim J, Park Y, Kim J, Lee S, Kim B and Jang B: Crude extract of Ceriporia lacerata has a protective effect on dexamethasone-induced cytotoxicity in INS-1 cells via the modulation of PI3K/PKB activity. Int J Mol Med 32: 179-186, 2013.
APA
Kim, J., Park, Y., Kim, J., Lee, S., Kim, B., & Jang, B. (2013). Crude extract of Ceriporia lacerata has a protective effect on dexamethasone-induced cytotoxicity in INS-1 cells via the modulation of PI3K/PKB activity. International Journal of Molecular Medicine, 32, 179-186. https://doi.org/10.3892/ijmm.2013.1364
MLA
Kim, J., Park, Y., Kim, J., Lee, S., Kim, B., Jang, B."Crude extract of Ceriporia lacerata has a protective effect on dexamethasone-induced cytotoxicity in INS-1 cells via the modulation of PI3K/PKB activity". International Journal of Molecular Medicine 32.1 (2013): 179-186.
Chicago
Kim, J., Park, Y., Kim, J., Lee, S., Kim, B., Jang, B."Crude extract of Ceriporia lacerata has a protective effect on dexamethasone-induced cytotoxicity in INS-1 cells via the modulation of PI3K/PKB activity". International Journal of Molecular Medicine 32, no. 1 (2013): 179-186. https://doi.org/10.3892/ijmm.2013.1364
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