Assessment of cardiac inflammation and remodeling during the development of streptozotocin-induced diabetic cardiomyopathy in vivo: A time course analysis

  • Authors:
    • P. M. Becher
    • D. Lindner
    • M. Fröhlich
    • K. Savvatis
    • D. Westermann
    • C. Tschöpe
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  • Published online on: May 2, 2013     https://doi.org/10.3892/ijmm.2013.1368
  • Pages: 158-164
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Abstract

In this study, we examined cardiac inflammation, fibrosis and left ventricular (LV) function during the development of streptozotocin (STZ)-induced diabetic cardiomyopathy using an animal model of diabetes mellitus (DM). Diabetes was induced in 22 Sprague‑Dawley rats by an intraperitoneal single injection of STZ (70 mg/kg). Non-diabetic animals served as the controls (n=6). LV function was documented using the conductance catheter technique 2 and 6 weeks after the induction of diabetes. Cardiac tissue was analyzed for cardiac immune cell infiltration, oxidative stress and remodeling in rats with STZ-induced diabetes at 2 different time points by immunohistochemistry. Cardiac function was significantly impaired in the diabetic animals. After 2 weeks, the induction of diabetes resulted in impaired cardiac function indexed by a decrease in systolic and diastolic LV function. This impairment of LV performance continued for up to 6 weeks after the STZ injection. This was associated with an increase in cardiac CD3+ and CD8a+ immune cell invasion and fibrosis, indexed by an increase in collagen content (p<0.05). Furthermore, oxidative stress response and matrix remodeling were increased after 2 weeks and this continued for up to 6 weeks after the induction of diabetes. In conclusion, cardiac dysfunction is associated with cardiac inflammation and adverse remodeling in experimental diabetic cardiomyopathy. Our results suggest that the model of STZ-induced diabetic cardiomyopathy is a robust model for investigating cardiac immune response and LV remodeling processes under diabetic conditions.
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July 2013
Volume 32 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Becher PM, Lindner D, Fröhlich M, Savvatis K, Westermann D and Tschöpe C: Assessment of cardiac inflammation and remodeling during the development of streptozotocin-induced diabetic cardiomyopathy in vivo: A time course analysis. Int J Mol Med 32: 158-164, 2013.
APA
Becher, P.M., Lindner, D., Fröhlich, M., Savvatis, K., Westermann, D., & Tschöpe, C. (2013). Assessment of cardiac inflammation and remodeling during the development of streptozotocin-induced diabetic cardiomyopathy in vivo: A time course analysis. International Journal of Molecular Medicine, 32, 158-164. https://doi.org/10.3892/ijmm.2013.1368
MLA
Becher, P. M., Lindner, D., Fröhlich, M., Savvatis, K., Westermann, D., Tschöpe, C."Assessment of cardiac inflammation and remodeling during the development of streptozotocin-induced diabetic cardiomyopathy in vivo: A time course analysis". International Journal of Molecular Medicine 32.1 (2013): 158-164.
Chicago
Becher, P. M., Lindner, D., Fröhlich, M., Savvatis, K., Westermann, D., Tschöpe, C."Assessment of cardiac inflammation and remodeling during the development of streptozotocin-induced diabetic cardiomyopathy in vivo: A time course analysis". International Journal of Molecular Medicine 32, no. 1 (2013): 158-164. https://doi.org/10.3892/ijmm.2013.1368