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International Journal of Molecular Medicine
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July 2013 Volume 32 Issue 1

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Article

Sann-Joong-Kuey-Jian-Tang decreases the protein expression of Mcl‑1 and TCTP and increases that of TNF-α and Bax in BxPC‑3 pancreatic carcinoma cells

  • Authors:
    • Su-Yu Chien
    • Shou-Jen Kuo
    • Dar-Ren Chen
    • Chin-Cheng Su
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Changhua Christian Hospital, Changhua 50006, Taiwan, R.O.C., Comprehensive Breast Cancer Center, Changhua Christian Hospital, Changhua 50006, Taiwan, R.O.C., Tumor Research Center of Integrative Medicine, Changhua Christian Hospital, Changhua 50006, Taiwan, R.O.C.
  • Pages: 85-92
    |
    Published online on: May 8, 2013
       https://doi.org/10.3892/ijmm.2013.1369
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Abstract

Sann-Joong-Kuey-Jian-Tang (SJKJT), a traditional Chinese medicinal prescription, has been used for the treatment of lymphadenopathy and solid tumors, and has shown therapeutic potential in several human malignant tumor cell lines. However, the efficacy and molecular mechanisms of action of SJKJT in human pancreatic cancer have not yet been elucidated. In the present study, we evaluated the cytotoxic effects of SJKJT on BxPC-3 human pancreatic carcinoma cells by MTT assay. The protein expression levels of myeloid cell leukemia 1 protein (Mcl-1), translationally controlled tumor protein (TCTP), tumor necrosis factor-α (TNF‑α), caspase-8, caspase-3, Bax and Bcl-2 family in the BxPC-3 cells were measured by western blot analysis. The cell cycle was analyzed by flow cytometry. The protein expression of caspase-3 was also detected by immunocytochemistry (ICC). The results revealed that SJKJT inhibited the proliferation of BxPC-3 cells in a time- and dose-dependent manner. The protein expression levels of TNF-α, caspase-8, caspase-3 and Bax increased in the BxPC-3 cells treated with SJKJT; however, the levels of Mcl-1, TCTP and Bcl-xL decreased. The results also demonstrated that SJKJT increased the percentage of BxPC-3 cells in the sub-G1 phase. In addition, ICC staining indicated that the protein expression of caspase-3 was upregulated in the BxPC-3 cells treated with SJKJT. These findings indicate that SJKJT inhibits the proliferation of BxPC-3 cells through the extrinsic and intrinsic pathway, inducing apoptosis in vitro. Our study, using BxPC-3 human pancreatic cancer cells, demonstrates that SJKJT has potential as a chemotherapeutic agent for the treatment of pancreatic cancer. Further sutdies are warranted to fully elucidate its mechanisms of action.
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Copy and paste a formatted citation
Spandidos Publications style
Chien S, Kuo S, Chen D and Su C: Sann-Joong-Kuey-Jian-Tang decreases the protein expression of Mcl‑1 and TCTP and increases that of TNF-α and Bax in BxPC‑3 pancreatic carcinoma cells. Int J Mol Med 32: 85-92, 2013.
APA
Chien, S., Kuo, S., Chen, D., & Su, C. (2013). Sann-Joong-Kuey-Jian-Tang decreases the protein expression of Mcl‑1 and TCTP and increases that of TNF-α and Bax in BxPC‑3 pancreatic carcinoma cells. International Journal of Molecular Medicine, 32, 85-92. https://doi.org/10.3892/ijmm.2013.1369
MLA
Chien, S., Kuo, S., Chen, D., Su, C."Sann-Joong-Kuey-Jian-Tang decreases the protein expression of Mcl‑1 and TCTP and increases that of TNF-α and Bax in BxPC‑3 pancreatic carcinoma cells". International Journal of Molecular Medicine 32.1 (2013): 85-92.
Chicago
Chien, S., Kuo, S., Chen, D., Su, C."Sann-Joong-Kuey-Jian-Tang decreases the protein expression of Mcl‑1 and TCTP and increases that of TNF-α and Bax in BxPC‑3 pancreatic carcinoma cells". International Journal of Molecular Medicine 32, no. 1 (2013): 85-92. https://doi.org/10.3892/ijmm.2013.1369
Copy and paste a formatted citation
x
Spandidos Publications style
Chien S, Kuo S, Chen D and Su C: Sann-Joong-Kuey-Jian-Tang decreases the protein expression of Mcl‑1 and TCTP and increases that of TNF-α and Bax in BxPC‑3 pancreatic carcinoma cells. Int J Mol Med 32: 85-92, 2013.
APA
Chien, S., Kuo, S., Chen, D., & Su, C. (2013). Sann-Joong-Kuey-Jian-Tang decreases the protein expression of Mcl‑1 and TCTP and increases that of TNF-α and Bax in BxPC‑3 pancreatic carcinoma cells. International Journal of Molecular Medicine, 32, 85-92. https://doi.org/10.3892/ijmm.2013.1369
MLA
Chien, S., Kuo, S., Chen, D., Su, C."Sann-Joong-Kuey-Jian-Tang decreases the protein expression of Mcl‑1 and TCTP and increases that of TNF-α and Bax in BxPC‑3 pancreatic carcinoma cells". International Journal of Molecular Medicine 32.1 (2013): 85-92.
Chicago
Chien, S., Kuo, S., Chen, D., Su, C."Sann-Joong-Kuey-Jian-Tang decreases the protein expression of Mcl‑1 and TCTP and increases that of TNF-α and Bax in BxPC‑3 pancreatic carcinoma cells". International Journal of Molecular Medicine 32, no. 1 (2013): 85-92. https://doi.org/10.3892/ijmm.2013.1369
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