Chrysanthemum zawadskii extract protects osteoblastic cells from highly reducing sugar-induced oxidative damage

Suh,Kwang  Sik; Rhee,Sang  Youl; Jung,Woon  Won; Kim,Nam  Jae; Jang,Young  Pyo; Kim,Hye  Jin; Kim,Min  Kyoung; Choi,Young  Kil; Kim,Young  Seol

doi:10.3892/ijmm.2013.1371

Chrysanthemum zawadskii extract protects osteoblastic cells from highly reducing sugar-induced oxidative damage

Authors:
- Kwang Sik Suh
- Sang Youl Rhee
- Woon Won Jung
- Nam Jae Kim
- Young Pyo Jang
- Hye Jin Kim
- Min Kyoung Kim
- Young Kil Choi
- Young Seol Kim
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Affiliations: Research Institute of Endocrinology, Kyung Hee University Hospital, Dongdaemun-gu, Seoul 130-702, Republic of Korea, Department of Endocrinology and Metabolism, School of Medicine, Kyung Hee University, Dongdaemun-gu, Seoul 130-701, Republic of Korea, Department of Biomedical Science, College of Health Sciences, Cheongju University, Sangdang-gu, Cheongju, Chungbuk 360-764, Republic of Korea, East-West Medical Research Institute, Kyung Hee University Hospital, Dongdaemun-gu, Seoul 130-702, Republic of Korea, Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University, Dongdaemun-gu, Seoul 130-701, Republic of Korea, Department of Endocrinology and Metabolism, Kangnam Cha Hospital, Kangnam-gu, Seoul 135-913, Republic of Korea
Published online on: May 8, 2013 https://doi.org/10.3892/ijmm.2013.1371
Pages: 241-250

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Abstract

In this study, Chrysanthemum zawadskii extract (CZE) was investigated to determine its effects on 2-deoxy-D-ribose (dRib)-induced oxidative damage and cellular dysfunction in the MC3T3-E1 mouse osteoblastic cell line. Osteoblastic cells were treated with the highly reducing sugar, dRib, in the presence or absence of CZE. Cell viability, apoptosis and reactive oxygen species (ROS) production were subsequently examined. It was observed that dRib reduced cell survival, while it markedly increased the intracellular levels of ROS and apoptosis. However, pre-treatment of the cells with CZE attenuated all the dRib-induced effects. The antioxidant, N-acetyl-L-cysteine (NAC), also prevented dRib-induced oxidative cell damage. In addition, treatment with CZE resulted in a significant increase in alkaline phosphatase (ALP) activity and collagen content, as well as in the expression of genes associated with osteoblast differentiation [ALP, collagen, osteopontin (OPN), osteoprotegerin (OPG), bone sialoprotein (BSP), osteocalcin (OC) and bone morphogenetic protein (BMP)2, BMP4 and BMP7]. In mechanistic studies of the antioxidative potential of CZE, we found that CZE reversed the dRib-induced decrease in the expression of phosphatidylinositol 3-kinase (PI3K) and protein kinase B (AKT)1 and AKT2 genes, which are master regulators of survival-related signaling pathways. CZE also upregulated the gene expression of the antioxidant enzymes, superoxide dismutase (SOD)2, SOD3 and glutathione peroxidase 4 (GPx4), which was inhibited by dRib. Taken together, these results suggest that CZE attenuates dRib-induced cell damage in osteoblastic cells and may be useful for the treatment of diabetes-associated bone disease.

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