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International Journal of Molecular Medicine
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Article

Directed neuronal differentiation of mouse embryonic and induced pluripotent stem cells and their gene expression profiles

  • Authors:
    • Xuesong Chen
    • Qi Gu
    • Xiang Wang
    • Qingwen Ma
    • Huixiang Tang
    • Xiaoshuang Yan
    • Xinbing Guo
    • Hao Yan
    • Jie Hao
    • Fanyi Zeng
  • View Affiliations / Copyright

    Affiliations: Laboratory of Developmental Biology, Institute of Medical Science, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China, College of Life Science, Northeast Agricultural University of China, Harbin 150030, P.R. China, Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai 200040, P.R. China, Shanghai Key Laboratory of Embryo and Reproduction Engineering and Key Laboratory of Embryo Molecular Biology, Ministry of Health, Shanghai 200040, P.R. China, State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China
  • Pages: 25-34
    |
    Published online on: May 8, 2013
       https://doi.org/10.3892/ijmm.2013.1372
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Abstract

Embryonic stem cells (ESCs) may be useful as a therapeutic source of cells for the production of healthy tissue; however, they are associated with certain challenges including immunorejection as well as ethical issues. Induced pluripotent stem cells (iPSCs) are a promising substitute since a patient's own adult cells would serve as tissue precursors. Ethical concerns prevent a full evaluation of the developmental potency of human ESCs and iPSCs, therefore, mouse iPSC models are required for protocol development and safety assessments. We used a modified culturing protocol to differentiate pluripotent cells from a mouse iPS cell line and two mouse ES cell lines into neurons. Our results indicated that all three pluripotent stem cell lines underwent nearly the same differentiation process when induced to form neurons in vitro. Genomic expression microarray profiling and single-cell RT-qPCR were used to analyze the neural lineage differentiation process, and more than one thousand differentially expressed genes involved in multiple molecular processes relevant to neural development were identified.
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Copy and paste a formatted citation
Spandidos Publications style
Chen X, Gu Q, Wang X, Ma Q, Tang H, Yan X, Guo X, Yan H, Hao J, Zeng F, Zeng F, et al: Directed neuronal differentiation of mouse embryonic and induced pluripotent stem cells and their gene expression profiles. Int J Mol Med 32: 25-34, 2013.
APA
Chen, X., Gu, Q., Wang, X., Ma, Q., Tang, H., Yan, X. ... Zeng, F. (2013). Directed neuronal differentiation of mouse embryonic and induced pluripotent stem cells and their gene expression profiles. International Journal of Molecular Medicine, 32, 25-34. https://doi.org/10.3892/ijmm.2013.1372
MLA
Chen, X., Gu, Q., Wang, X., Ma, Q., Tang, H., Yan, X., Guo, X., Yan, H., Hao, J., Zeng, F."Directed neuronal differentiation of mouse embryonic and induced pluripotent stem cells and their gene expression profiles". International Journal of Molecular Medicine 32.1 (2013): 25-34.
Chicago
Chen, X., Gu, Q., Wang, X., Ma, Q., Tang, H., Yan, X., Guo, X., Yan, H., Hao, J., Zeng, F."Directed neuronal differentiation of mouse embryonic and induced pluripotent stem cells and their gene expression profiles". International Journal of Molecular Medicine 32, no. 1 (2013): 25-34. https://doi.org/10.3892/ijmm.2013.1372
Copy and paste a formatted citation
x
Spandidos Publications style
Chen X, Gu Q, Wang X, Ma Q, Tang H, Yan X, Guo X, Yan H, Hao J, Zeng F, Zeng F, et al: Directed neuronal differentiation of mouse embryonic and induced pluripotent stem cells and their gene expression profiles. Int J Mol Med 32: 25-34, 2013.
APA
Chen, X., Gu, Q., Wang, X., Ma, Q., Tang, H., Yan, X. ... Zeng, F. (2013). Directed neuronal differentiation of mouse embryonic and induced pluripotent stem cells and their gene expression profiles. International Journal of Molecular Medicine, 32, 25-34. https://doi.org/10.3892/ijmm.2013.1372
MLA
Chen, X., Gu, Q., Wang, X., Ma, Q., Tang, H., Yan, X., Guo, X., Yan, H., Hao, J., Zeng, F."Directed neuronal differentiation of mouse embryonic and induced pluripotent stem cells and their gene expression profiles". International Journal of Molecular Medicine 32.1 (2013): 25-34.
Chicago
Chen, X., Gu, Q., Wang, X., Ma, Q., Tang, H., Yan, X., Guo, X., Yan, H., Hao, J., Zeng, F."Directed neuronal differentiation of mouse embryonic and induced pluripotent stem cells and their gene expression profiles". International Journal of Molecular Medicine 32, no. 1 (2013): 25-34. https://doi.org/10.3892/ijmm.2013.1372
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