COX-2 regulates E-cadherin expression through the NF-κB/Snail signaling pathway in gastric cancer Corrigendum in /10.3892/ijmm.2022.5083

Chen,Zhaofeng; Liu,Min; Liu,Xiaojun; Huang,Shanshan; Li,Linlin; Song,Bo; Li,Hailong; Ren,Qian; Hu,Zenan; Zhou,Yongning; Qiao,Liang

doi:10.3892/ijmm.2013.1376

July 2013 Volume 32 Issue 1

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July 2013 Volume 32 Issue 1

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Article

COX-2 regulates E-cadherin expression through the NF-κB/Snail signaling pathway in gastric cancer

Corrigendum in: /10.3892/ijmm.2022.5083

Authors:
- Zhaofeng Chen
- Min Liu
- Xiaojun Liu
- Shanshan Huang
- Linlin Li
- Bo Song
- Hailong Li
- Qian Ren
- Zenan Hu
- Yongning Zhou
- Liang Qiao
View Affiliations / Copyright

Affiliations: Division of Gastroenterology and Hepatology, The First Hospital of Lanzhou University, Lanzhou, Gansu, P.R. China, Division of Gastroenterology and Hepatology, Dongying District Hospital, Dongying, Shandong, P.R. China, Storr Liver Unit, Westmead Millennium Institute, Western Clinical School, Faculty of Medicine, Westmead Hospital, University of Sydney, Westmead, NSW, Australia
Pages: 93-100
|
Published online on: May 10, 2013

https://doi.org/10.3892/ijmm.2013.1376
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Abstract

Cyclooxygenase-2 (COX-2) participates in cancer invasion and metastasis by decreasing the expression of E-cadherin. However, the molecular mechanisms through which COX-2 regulates E-cadherin expression and function have not yet been fully elucidated. The aim of this study was to investigate the possible molecular mechanisms through which COX-2 regulates E-cadherin expression in gastric cancer. The mRNA and protein expression of COX-2, nuclear factor-κB (NF-κB), Snail and E-cadherin was detected in gastric cancer cells by quantitative PCR and western blot analysis, respectively. The expression of these genes was also detected in healthy gastric mucosa and gastric cancer tissues by immunohistochemistry. We detected various levels of COX-2, nuclear factor-κB (NF-κB), Snail and E-cadherin expression in the normal gastric mucosa and cancer tissues; however, the expression patterns differed: the increased expression of COX-2, NF-κB and Snail was observed in the gastric cancer tissues, whereas there was a considerable reduction in E-cadherin expression in the cancer tissues compared to the normal gastric mucosa. The expression patterns of COX-2, NF-κB and Snail were similar. The increased expression of COX-2 in the gastric cancer tissues closely correlated with the increased expression of NF-κB and Snail, but inversely correlated with the expression of E-cadherin. Treatment of the SGC7901 cells (which express high levels of COX-2) with celecoxib, a COX-2 inhibitor, not only led to a marked dose- and time-dependent decrease in the expression of COX-2, NF-κB and Snail, but also led to a significant increase in the expression of E-cadherin, and this was associated with a reduction in cell invasion. By contrast, the same treatment did not alter the expression of these genes in another gastric cancer cell line, MGC803 (which barely expresses COX-2). These data suggest that COX-2 regulates the expression of E-cadherin through the NF-κB and Snail signaling pathway in gastric cancer.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

COX-2 regulates E-cadherin expression through the NF-κB/Snail signaling pathway in gastric cancer

Corrigendum in: /10.3892/ijmm.2022.5083

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Abstract

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