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Article

Differential expression of miRNAs in the nervous system of a rat model of bilateral sciatic nerve chronic constriction injury

  • Authors:
    • Haixia Li
    • Le Shen
    • Chao Ma
    • Yuguang Huang
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Peking Union Medical College Hospital, Beijing 100730, P.R. China, Department of Anatomy, Histology and Embryology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing 100730, P.R. China
  • Pages: 219-226
    |
    Published online on: May 14, 2013
       https://doi.org/10.3892/ijmm.2013.1381
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Abstract

Chronic neuropathic pain is associated with global changes in gene expression in different areas of the nociceptive pathway. MicroRNAs (miRNAs) are small (~22 nt long) non-coding RNAs, which are able to regulate hundreds of different genes post-transcriptionally. The aim of this study was to determine the miRNA expression patterns in the different regions of the pain transmission pathway using a rat model of human neuropathic pain induced by bilateral sciatic nerve chronic constriction injury (bCCI). Using microarray analysis and quantitative reverse transcriptase-PCR, we observed a significant upregulation in miR-341 expression in the dorsal root ganglion (DRG), but not in the spinal dorsal horn (SDH), hippocampus or anterior cingulate cortex (ACC), in the rats with neuropathic pain compared to rats in the naïve and sham-operated groups. By contrast, the expression of miR-203, miR-181a-1* and miR-541* was significantly reduced in the SDH of rats with neuropathic pain. Our data indicate that miR-341 is upregulated in the DRG, whereas miR-203, miR-181a-1* and miR-541* are downregulated in the SDH under neuropathic pain conditions. Thus, the differential expression of miRNAs in the nervous system may play a role in the development of chronic pain. These observations may aid in the development of novel treatment methods for neuropathic pain, which may involve miRNA gene therapy in local regions.
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View References

1. 

Treede RD, Jensen TS, Campbell JN, et al: Neuropathic pain: redefinition and a grading system for clinical and research purposes. Neurology. 70:1630–1635. 2008. View Article : Google Scholar : PubMed/NCBI

2. 

Haanpää M, Attal N, Backonja M, et al: NeuPSIG guidelines on neuropathic pain assessment. Pain. 152:14–27. 2011.

3. 

Green L and McGhie J: Assessment of acute and chronic pain. Anaesth Intensive Care Med. 12:9–11. 2011. View Article : Google Scholar

4. 

Bie B, Brown DL and Naguib M: Increased synaptic GluR1 subunits in the anterior cingulate cortex of rats with peripheral inflammation. Eur J Pharmacol. 653:26–31. 2011. View Article : Google Scholar : PubMed/NCBI

5. 

Boroujerdi A, Zeng J, Sharp K, Kim D, Steward O and Luo ZD: Calcium channel alpha-2-delta-1 protein upregulation in dorsal spinal cord mediates spinal cord injury-induced neuropathic pain states. Pain. 152:649–655. 2011. View Article : Google Scholar : PubMed/NCBI

6. 

Chou CW, Wong GT, Lim G, et al: Peripheral nerve injury alters the expression of NF-kappaB in the rat’s hippocampus. Brain Res. 1378:66–71. 2011.PubMed/NCBI

7. 

Emery EC, Young GT, Berrocoso EM, Chen L and McNaughton PA: HCN2 ion channels play a central role in inflammatory and neuropathic pain. Science. 333:1462–1466. 2011. View Article : Google Scholar : PubMed/NCBI

8. 

Jaggi AS and Singh N: Role of different brain areas in peripheral nerve injury-induced neuropathic pain. Brain Res. 1381:187–201. 2011. View Article : Google Scholar : PubMed/NCBI

9. 

Vogt BA: Pain and emotion interactions in subregions of the cingulate gyrus. Nat Rev Neurosci. 6:533–544. 2005. View Article : Google Scholar : PubMed/NCBI

10. 

Hodgdon KE, Hingtgen CM and Nicol GD: Dorsal root ganglia isolated from Nf1+/− mice exhibit increased levels of mRNA expression of voltage-dependent sodium channels. Neuroscience. 206:237–244. 2012.PubMed/NCBI

11. 

Kim DS, Figueroa KW, Li KW, Boroujerdi A, Yolo T and Luo ZD: Profiling of dynamically changed gene expression in dorsal root ganglia post peripheral nerve injury and a critical role of injury-induced glial fibrillary acetic protein in maintenance of pain behaviors. Pain. 143:114–122. 2009. View Article : Google Scholar : PubMed/NCBI

12. 

Cady RJ, Glenn JR, Smith KM and Durham PL: Calcitonin gene-related peptide promotes cellular changes in trigeminal neurons and glia implicated in peripheral and central sensitization. Mol Pain. 7:942011. View Article : Google Scholar : PubMed/NCBI

13. 

Uchida H, Ma L and Ueda H: Epigenetic gene silencing underlies C-fiber dysfunctions in neuropathic pain. J Neurosci. 30:4806–4814. 2010. View Article : Google Scholar : PubMed/NCBI

14. 

Aldrich BT, Frakes EP, Kasuya J, Hammond DL and Kitamoto T: Changes in expression of sensory organ-specific microRNAs in rat dorsal root ganglia in association with mechanical hyper-sensitivity induced by spinal nerve ligation. Neuroscience. 164:711–723. 2009. View Article : Google Scholar : PubMed/NCBI

15. 

Bak M, Silahtaroglu A, Moller M, et al: MicroRNA expression in the adult mouse central nervous system. RNA. 14:432–444. 2008. View Article : Google Scholar : PubMed/NCBI

16. 

Bastian I, Tam Tam S, Zhou X-F, et al: Differential expression of microRNA-1 in dorsal root ganglion neurons. Histochem Cell Biol. 135:37–45. 2010. View Article : Google Scholar : PubMed/NCBI

17. 

von Schack D, Agostino MJ, Murray BS, et al: Dynamic changes in the microRNA expression profile reveal multiple regulatory mechanisms in the spinal nerve ligation model of neuropathic pain. PLoS One. 6:e176702011.PubMed/NCBI

18. 

Chiang HR, Schoenfeld LW, Ruby JG, et al: Mammalian microRNAs: experimental evaluation of novel and previously annotated genes. Genes Dev. 24:992–1009. 2010. View Article : Google Scholar : PubMed/NCBI

19. 

Hua YJ, Tang ZY, Tu K, et al: Identification and target prediction of miRNAs specifically expressed in rat neural tissue. BMC Genomics. 10:2142009. View Article : Google Scholar : PubMed/NCBI

20. 

Kosik KS: The neuronal microRNA system. Nat Rev Neurosci. 7:911–920. 2006. View Article : Google Scholar : PubMed/NCBI

21. 

Wheeler G, Ntounia-Fousara S, Granda B, Rathjen T and Dalmay T: Identification of new central nervous system specific mouse microRNAs. FEBS Lett. 580:2195–2200. 2006. View Article : Google Scholar : PubMed/NCBI

22. 

Kusuda R, Cadetti F, Ravanelli MI, et al: Differential expression of microRNAs in mouse pain models. Mol Pain. 7:172011. View Article : Google Scholar : PubMed/NCBI

23. 

Harraz MM, Dawson TM and Dawson VL: MicroRNAs in Parkinson’s disease. J Chem Neuroanat. 42:127–130. 2011.

24. 

Zhang HY, Zheng SJ, Zhao JH, et al: MicroRNAs 144, 145, and 214 are down-regulated in primary neurons responding to sciatic nerve transection. Brain Res. 1383:62–70. 2011. View Article : Google Scholar : PubMed/NCBI

25. 

Datta S, Chatterjee K, Kline RH and Wiley RG: Behavioral and anatomical characterization of the bilateral sciatic nerve chronic constriction (bCCI) injury: correlation of anatomic changes and responses to cold stimuli. Mol Pain. 6:72010. View Article : Google Scholar

26. 

Vierck CJ, Acosta-Rua AJ and Johnson RD: Bilateral chronic constriction of the sciatic nerve: a model of long-term cold hyper-algesia. J Pain. 6:507–517. 2005. View Article : Google Scholar : PubMed/NCBI

27. 

Chaplan SR, Bach FW, Pogrel JW, Chung JM and Yaksh TL: Quantitative assessment of tactile allodynia in the rat paw. J Neurosci Methods. 53:55–63. 1994. View Article : Google Scholar : PubMed/NCBI

28. 

Schmittgen TD and Livak KJ: Analyzing real-time PCR data by the comparative CT method. Nat Protoc. 3:1101–1108. 2008. View Article : Google Scholar : PubMed/NCBI

29. 

Krichevsky AM, King KS, Donahue CP, Khrapko K and Kosik KS: A microRNA array reveals extensive regulation of microRNAs during brain development. RNA. 9:1274–1281. 2003. View Article : Google Scholar : PubMed/NCBI

30. 

Ambros V: The functions of animal microRNAs. Nature. 431:350–355. 2004. View Article : Google Scholar : PubMed/NCBI

31. 

Schratt GM, Tuebing F, Nigh EA, et al: A brain-specific microRNA regulates dendritic spine development. Nature. 439:283–289. 2006. View Article : Google Scholar : PubMed/NCBI

32. 

Mercader JM, Gonzalez JR, Lozano JJ, et al: Aberrant brain microRNA target and miRISC gene expression in the anx/anx anorexia mouse model. Gene. 497:181–190. 2012. View Article : Google Scholar : PubMed/NCBI

33. 

Wei L, Wang M, Qu X, et al: Differential expression of microRNAs during allograft rejection. Am J Transplant. 12:1113–1123. 2012. View Article : Google Scholar : PubMed/NCBI

34. 

Liu PT, Wheelwright M, Teles R, et al: MicroRNA-21 targets the vitamin D-dependent antimicrobial pathway in leprosy. Nat Med. 18:267–273. 2012. View Article : Google Scholar : PubMed/NCBI

35. 

Kocerha J, Faghihi MA, Lopez-Toledano MA, et al: MicroRNA-219 modulates NMDA receptor-mediated neurobehavioral dysfunction. Proc Natl Acad Sci USA. 106:3507–3512. 2009. View Article : Google Scholar : PubMed/NCBI

36. 

Zhao J, Lee MC, Momin A, et al: Small RNAs control sodium channel expression, nociceptor excitability, and pain thresholds. J Neurosci. 30:10860–10871. 2010. View Article : Google Scholar : PubMed/NCBI

37. 

Kuner R: Central mechanisms of pathological pain. Nature Med. 16:1258–1266. 2010. View Article : Google Scholar : PubMed/NCBI

38. 

Gold MS and Gebhart GF: Nociceptor sensitization in pain pathogenesis. Nature Med. 16:1248–1257. 2010. View Article : Google Scholar : PubMed/NCBI

39. 

Ruangsri S, Lin A, Mulpuri Y, Lee K, Spigelman I and Nishimura I: Relationship of axonal voltage-gated sodium channel 1.8 (NaV1.8) mRNA accumulation to sciatic nerve injury-induced painful neuropathy in rats. J Biol Chem. 286:39836–39847. 2011. View Article : Google Scholar : PubMed/NCBI

40. 

Huang B, Zhao X, Zheng LB, Zhang L, Ni B and Wang YW: Different expression of tissue inhibitor of metalloproteinase family members in rat dorsal root ganglia and their changes after peripheral nerve injury. Neuroscience. 193:421–428. 2011. View Article : Google Scholar

41. 

Koturbash I, Zemp F, Kolb B and Kovalchuk O: Sex-specific radiation-induced microRNAome responses in the hippocampus, cerebellum and frontal cortex in a mouse model. Mutat Res. 722:114–118. 2011. View Article : Google Scholar : PubMed/NCBI

42. 

Rao MK, Pham J, Imam JS, et al: Tissue-specific RNAi reveals that WT1 expression in nurse cells controls germ cell survival and spermatogenesis. Genes Dev. 20:147–152. 2006. View Article : Google Scholar : PubMed/NCBI

43. 

Bo J, Yang G, Huo K, et al: microRNA-203 suppresses bladder cancer development by repressing bcl-w expression. FEBS J. 278:786–792. 2011. View Article : Google Scholar : PubMed/NCBI

44. 

Elmén J, Lindow M, Schütz S, et al: LNA-mediated microRNA silencing in non-human primates. Nature. 452:896–899. 2008.PubMed/NCBI

45. 

Broderick JA and Zamore PD: MicroRNA therapeutics. Gene Ther. 18:1104–1110. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li H, Shen L, Ma C and Huang Y: Differential expression of miRNAs in the nervous system of a rat model of bilateral sciatic nerve chronic constriction injury. Int J Mol Med 32: 219-226, 2013.
APA
Li, H., Shen, L., Ma, C., & Huang, Y. (2013). Differential expression of miRNAs in the nervous system of a rat model of bilateral sciatic nerve chronic constriction injury. International Journal of Molecular Medicine, 32, 219-226. https://doi.org/10.3892/ijmm.2013.1381
MLA
Li, H., Shen, L., Ma, C., Huang, Y."Differential expression of miRNAs in the nervous system of a rat model of bilateral sciatic nerve chronic constriction injury". International Journal of Molecular Medicine 32.1 (2013): 219-226.
Chicago
Li, H., Shen, L., Ma, C., Huang, Y."Differential expression of miRNAs in the nervous system of a rat model of bilateral sciatic nerve chronic constriction injury". International Journal of Molecular Medicine 32, no. 1 (2013): 219-226. https://doi.org/10.3892/ijmm.2013.1381
Copy and paste a formatted citation
x
Spandidos Publications style
Li H, Shen L, Ma C and Huang Y: Differential expression of miRNAs in the nervous system of a rat model of bilateral sciatic nerve chronic constriction injury. Int J Mol Med 32: 219-226, 2013.
APA
Li, H., Shen, L., Ma, C., & Huang, Y. (2013). Differential expression of miRNAs in the nervous system of a rat model of bilateral sciatic nerve chronic constriction injury. International Journal of Molecular Medicine, 32, 219-226. https://doi.org/10.3892/ijmm.2013.1381
MLA
Li, H., Shen, L., Ma, C., Huang, Y."Differential expression of miRNAs in the nervous system of a rat model of bilateral sciatic nerve chronic constriction injury". International Journal of Molecular Medicine 32.1 (2013): 219-226.
Chicago
Li, H., Shen, L., Ma, C., Huang, Y."Differential expression of miRNAs in the nervous system of a rat model of bilateral sciatic nerve chronic constriction injury". International Journal of Molecular Medicine 32, no. 1 (2013): 219-226. https://doi.org/10.3892/ijmm.2013.1381
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