Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
July 2013 Volume 32 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July 2013 Volume 32 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Expression and distribution of Src in the nucleus of myocytes in cardiac hypertrophy

  • Authors:
    • Ping Chen
    • Faqian Li
    • Zeqing Xu
    • Zhanyu Li
    • Xian Ping Yi
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, Guangdong 519000, P.R. China, Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA
  • Pages: 165-173
    |
    Published online on: May 14, 2013
       https://doi.org/10.3892/ijmm.2013.1382
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The Src kinase is involved in signaling events leading to cardiac hypertrophy. The exact effects of tyrosine phosphorylation and subnuclear distribution on cardiac hypertrophy and failure remain to be investigated. In this study, we examined the intranuclear expression and distribution of c-Src, Src phosphorylated at tyrosine 529 (Src[pY529]), Src phosphorylated at tyrosine 418 (Src[pY418]) and Src phosphorylated at tyrosine 215 (Src[pY215]) in the myocardial nuclei of the left ventricle (LV) from 2-, 6-, 12- and 18-month-old spontaneously hypertensive heart failure (SHHF) rats and age-matched Wistar-Kyoto (WKY) rats as normotensive controls by western blot analysis, immunofluorescent labeling and immunoprecipitation. Cellular Src (c-Src) expression in the myocardial nuclei of the LV of the 2-, 6-, 12- and 18-month-old SHHF rats was not significantly different from that in the myocardial nuclei of the LV of the age-matched WKY rats. Although there were no significant differences observed between the levels of Src[pY529] and Src[pY418] in the myocardial nuclei of the LV of the 2-month-old SHHF and WKY rats, the expression of Src[pY529] significantly decreased, while that of Src[pY418] significantly increased in the myocardial nuclei of the LV of the 6-, 12- and 18-month-old SHHF rats compared to the age-matched WKY controls. Furthermore, as demonstrated by double labeling with antibodies against fibrillarin and Src-associated in mitosis 68 kDa (Sam68), c-Src was co-localized with both Sam68 and fibrillarin in the nuclei; Src[pY529] co-localized with fibrillarin, but Src[pY418] co-localized with Sam68. The results from the present study suggest that the dephosphorylation of Src tyrosine kinase 529, the phosphorylation of tyrosine 418 and their subnuclear redistribution are involved in endonuclear signal transduction in cardiac myocytes, which regulates the development and progression of LV eccentric hypertrophy induced by hypertension.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1. 

Drazner MH: The progression of hypertensive heart disease. Circulation. 123:327–334. 2011. View Article : Google Scholar : PubMed/NCBI

2. 

Diamond JA and Phillips RA: Hypertensive heart disease. Hypertens Res. 28:191–202. 2005. View Article : Google Scholar

3. 

Krauser DG and Devereux RB: Ventricular hypertrophy and hypertension: prognostic elements and implications for management. Herz. 31:305–316. 2006. View Article : Google Scholar : PubMed/NCBI

4. 

Marin TM, Clemente CF, Santos AM, et al: Shp2 negatively regulates growth in cardiomyocytes by controlling focal adhesion kinase/Src and mTOR pathways. Circ Res. 103:813–824. 2008. View Article : Google Scholar : PubMed/NCBI

5. 

Torsoni AS, Constancio SS, Nadruz W Jr, Hanks SK and Franchini KG: Focal adhesion kinase is activated and mediates the early hypertrophic response to stretch in cardiac myocytes. Circ Res. 93:140–147. 2003. View Article : Google Scholar : PubMed/NCBI

6. 

Kuppuswamy D, Kerr C, Narishige T, Kasi VS, Menick DR and Cooper G IV: Association of tyrosine-phosphorylated c-Src with the cytoskeleton of hypertrophying myocardium. J Biol Chem. 272:4500–4508. 1997. View Article : Google Scholar : PubMed/NCBI

7. 

Kovacic B, Ilic D, Damsky CH and Gardner DG: c-Src activation plays a role in endothelin-dependent hypertrophy of the cardiac myocyte. J Biol Chem. 273:35185–35193. 1998.PubMed/NCBI

8. 

Zou Y, Komuro I, Yamazaki T, et al: Both Gs and Gi proteins are critically involved in isoproterenol-induced cardiomyocyte hypertrophy. J Biol Chem. 274:9760–9770. 1999. View Article : Google Scholar : PubMed/NCBI

9. 

Heidkamp MC, Bayer AL, Scully BT, Eble DM and Samarel AM: Activation of focal adhesion kinase by protein kinase C epsilon in neonatal rat ventricular myocytes. Am J Physiol Heart Circ Physiol. 285:H1684–H1696. 2003. View Article : Google Scholar : PubMed/NCBI

10. 

Aikawa R, Nagai T, Kudoh S, et al: Integrins play a critical role in mechanical stress-induced p38 MAPK activation. Hypertension. 39:233–238. 2002. View Article : Google Scholar : PubMed/NCBI

11. 

Roskoski R Jr: Src protein-tyrosine kinase structure and regulation. Biochem Biophys Res Commun. 324:1155–1164. 2004. View Article : Google Scholar : PubMed/NCBI

12. 

Roskoski R Jr: Src kinase regulation by phosphorylation and dephosphorylation. Biochem Biophys Res Commun. 331:1–14. 2005. View Article : Google Scholar : PubMed/NCBI

13. 

Li LH, Wang XH, Xie YY, et al: Membrane translocation of Src kinase in cardiac myocytes of hypertrophic left ventricle of hypertensive rats. Chinese Heart Journal. 23:295–299. 2011.(In Chinese).

14. 

Li ZY, Yi XP, Zhong L, et al: Expression of focal adhesion kinase in cardiac myocytes of hypertrophic ventricle. Zhonghua Bing Li Xue Za Zhi. 36:677–680. 2007.(In Chinese).

15. 

Zhong L, Yi XP, Li ZY and Faqian L: Phosphorylation and nuclear translocation of serine 722 and serine 910 of focal adhesion kinase in hypertrophic cardiac myocytes of left ventricle of spontaneously hypertensive rats. Zhonghua Bing Li Xue Za Zhi. 37:328–332. 2008.(In Chinese).

16. 

Yi XP, Gerdes AM and Li F: Myocyte redistribution of GRK2 and GRK5 in hypertensive, heart-failure-prone rats. Hypertension. 39:1058–1063. 2002. View Article : Google Scholar : PubMed/NCBI

17. 

Blough E, Dineen B and Esser K: Extraction of nuclear proteins from striated muscle tissue. Biotechniques. 26:202–204. 206:1999

18. 

Vielreicher M, Harms G, Butt E, Walter U and Obergfell A: Dynamic interaction between Src and C-terminal Src kinase in integrin alphaIIbbeta3-mediated signaling to the cytoskeleton. J Biol Chem. 282:33623–33631. 2007. View Article : Google Scholar : PubMed/NCBI

19. 

Papadopoulos DP and Papademetriou V: Hypertrophic and hypertensive hypertrophic cardiomyopathy - a true association? Angiology. 61:92–99. 2010. View Article : Google Scholar : PubMed/NCBI

20. 

Li F, Wang X, Yi XP and Gerdes AM: Structural basis of ventricular remodeling: role of the myocyte. Curr Heart Fail Rep. 1:5–8. 2004. View Article : Google Scholar

21. 

Heyen JR, Blasi ER, Nikula K, et al: Structural, functional, and molecular characterization of the SHHF model of heart failure. Am J Physiol Heart Circ Physiol. 283:H1775–H1784. 2002. View Article : Google Scholar : PubMed/NCBI

22. 

Johnsen DD, Kacimi R, Anderson BE, Thomas TA, Said S and Gerdes AM: Protein kinase C isozymes in hypertension and hypertrophy: insight from SHHF rat hearts. Mol Cell Biochem. 270:63–69. 2005. View Article : Google Scholar : PubMed/NCBI

23. 

Ingley E: Src family kinases: regulation of their activities, levels and identification of new pathways. Biochim Biophys Acta. 1784:56–65. 2008. View Article : Google Scholar : PubMed/NCBI

24. 

Schaller MD, Hildebrand JD and Parsons JT: Complex formation with focal adhesion kinase: A mechanism to regulate activity and subcellular localization of Src kinases. Mol Biol Cell. 10:3489–3505. 1999. View Article : Google Scholar : PubMed/NCBI

25. 

Amin MA, Matsunaga S, Ma N, et al: Fibrillarin, a nucleolar protein, is required for normal nuclear morphology and cellular growth in HeLa cells. Biochem Biophys Res Commun. 360:320–326. 2007. View Article : Google Scholar : PubMed/NCBI

26. 

Yanagida M, Hayano T, Yamauchi Y, et al: Human fibrillarin forms a sub-complex with splicing factor 2-associated p32, protein arginine methyltransferases, and tubulins alpha 3 and beta 1 that is independent of its association with preribosomal ribonucleoprotein complexes. J Biol Chem. 279:1607–1614. 2004. View Article : Google Scholar

27. 

Barygina VV, Veiko VP and Zatsepina OV: Analysis of nucleolar protein fibrillarin mobility and functional state in living HeLa cells. Biochemistry (Mosc). 75:979–988. 2010. View Article : Google Scholar : PubMed/NCBI

28. 

Rajan P, Gaughan L, Dalgliesh C, et al: Regulation of gene expression by the RNA-binding protein Sam68 in cancer. Biochem Soc Trans. 36:505–507. 2008. View Article : Google Scholar : PubMed/NCBI

29. 

Bielli P, Busa R, Paronetto MP and Sette C: The RNA-binding protein Sam68 is a multifunctional player in human cancer. Endocr Relat Cancer. 18:R91–R102. 2011. View Article : Google Scholar : PubMed/NCBI

30. 

Najib S, Martin-Romero C, Gonzalez-Yanes C and Sanchez-Margalet V: Role of Sam68 as an adaptor protein in signal transduction. Cell Mol Life Sci. 62:36–43. 2005. View Article : Google Scholar : PubMed/NCBI

31. 

Sette C: Post-translational regulation of star proteins and effects on their biological functions. Adv Exp Med Biol. 693:54–66. 2010. View Article : Google Scholar : PubMed/NCBI

32. 

Fuller SJ, Gillespie-Brown J and Sugden PH: Oncogenic src, raf, and ras stimulate a hypertrophic pattern of gene expression and increase cell size in neonatal rat ventricular myocytes. J Biol Chem. 273:18146–18152. 1998. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen P, Li F, Xu Z, Li Z and Yi XP: Expression and distribution of Src in the nucleus of myocytes in cardiac hypertrophy. Int J Mol Med 32: 165-173, 2013.
APA
Chen, P., Li, F., Xu, Z., Li, Z., & Yi, X.P. (2013). Expression and distribution of Src in the nucleus of myocytes in cardiac hypertrophy. International Journal of Molecular Medicine, 32, 165-173. https://doi.org/10.3892/ijmm.2013.1382
MLA
Chen, P., Li, F., Xu, Z., Li, Z., Yi, X. P."Expression and distribution of Src in the nucleus of myocytes in cardiac hypertrophy". International Journal of Molecular Medicine 32.1 (2013): 165-173.
Chicago
Chen, P., Li, F., Xu, Z., Li, Z., Yi, X. P."Expression and distribution of Src in the nucleus of myocytes in cardiac hypertrophy". International Journal of Molecular Medicine 32, no. 1 (2013): 165-173. https://doi.org/10.3892/ijmm.2013.1382
Copy and paste a formatted citation
x
Spandidos Publications style
Chen P, Li F, Xu Z, Li Z and Yi XP: Expression and distribution of Src in the nucleus of myocytes in cardiac hypertrophy. Int J Mol Med 32: 165-173, 2013.
APA
Chen, P., Li, F., Xu, Z., Li, Z., & Yi, X.P. (2013). Expression and distribution of Src in the nucleus of myocytes in cardiac hypertrophy. International Journal of Molecular Medicine, 32, 165-173. https://doi.org/10.3892/ijmm.2013.1382
MLA
Chen, P., Li, F., Xu, Z., Li, Z., Yi, X. P."Expression and distribution of Src in the nucleus of myocytes in cardiac hypertrophy". International Journal of Molecular Medicine 32.1 (2013): 165-173.
Chicago
Chen, P., Li, F., Xu, Z., Li, Z., Yi, X. P."Expression and distribution of Src in the nucleus of myocytes in cardiac hypertrophy". International Journal of Molecular Medicine 32, no. 1 (2013): 165-173. https://doi.org/10.3892/ijmm.2013.1382
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team