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International Journal of Molecular Medicine
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August 2013 Volume 32 Issue 2

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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Article

Kinase inhibitors fail to induce mesenchymal-epithelial transition in fibroblasts from fibrotic lung tissue

Corrigendum in: /10.3892/ijmm.2020.4660
  • Authors:
    • Xuefeng Xu
    • Xuan Wan
    • Jing Geng
    • Fei Li
    • Chen Wang
    • Huaping Dai
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory and Critical Care Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, P.R. China, Beijing Key Laboratory of Respiratory and Pulmonary Circulation Disorders, Beijing Institute of Respiratory Medicine, Beijing 100020, P.R. China
  • Pages: 430-438
    |
    Published online on: June 11, 2013
       https://doi.org/10.3892/ijmm.2013.1415
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Abstract

Epithelial-mesenchymal transition (EMT) has been considered to be involved in idiopathic pulmonary fibrosis (IPF). However, the EMT process in vivo is much more complex and controversial. Studies regarding the opposite process, mesenchymal-epithelial transition (MET) in fibroblasts, are limited. Therefore, the aim of this study was to verify the involvement of the transforming growth factor (TGF)-β1-dependent EMT network in the process of pulmonary fibrosis and to explore the possibility of MET. Fibrotic lung tissues were obtained from patients with IPF with histological evidence of usual interstitial pneumonia at the time of surgical lung biopsy. For the controls, histologically normal lung tissues were obtained from patients with primary spontaneous pneumothorax at the time of thoracoscopy with stapling of any air leaks. Real-time RT-PCR and western blot analysis revealed that the mRNA and protein levels of TGF-β1, TGF-β1 receptor type I/II/III (TβRI/II/III), Smad2/3/4 and Snail1/2 were significantly upregulated in the fibrotic lung tissue. Inhibitors of various kinases implicated in EMT, including TGF-β1/Smad, Rho kinase (ROCK), p38 mitogen-activated protein kinase (p38 MAPK) and c-Jun NH-terminal kinase (JNK) were used to determine the MET potential in fibroblasts from fibrotic lung tissue. Western blot analysis or indirect immunofluorescence staining revealed that Smad inhibitor, as well as other kinase inhibitors failed to induce the MET process, determined by cellular morphology and protein markers. Our data suggest that the MET process may not be the exact reversal of EMT. In addition to using kinase inhibitors, other intervention measures should be used to explore the possibility of the MET process in fibroblasts from fibrotic lung tissue.
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Copy and paste a formatted citation
Spandidos Publications style
Xu X, Wan X, Geng J, Li F, Wang C and Dai H: Kinase inhibitors fail to induce mesenchymal-epithelial transition in fibroblasts from fibrotic lung tissue Corrigendum in /10.3892/ijmm.2020.4660. Int J Mol Med 32: 430-438, 2013.
APA
Xu, X., Wan, X., Geng, J., Li, F., Wang, C., & Dai, H. (2013). Kinase inhibitors fail to induce mesenchymal-epithelial transition in fibroblasts from fibrotic lung tissue Corrigendum in /10.3892/ijmm.2020.4660. International Journal of Molecular Medicine, 32, 430-438. https://doi.org/10.3892/ijmm.2013.1415
MLA
Xu, X., Wan, X., Geng, J., Li, F., Wang, C., Dai, H."Kinase inhibitors fail to induce mesenchymal-epithelial transition in fibroblasts from fibrotic lung tissue Corrigendum in /10.3892/ijmm.2020.4660". International Journal of Molecular Medicine 32.2 (2013): 430-438.
Chicago
Xu, X., Wan, X., Geng, J., Li, F., Wang, C., Dai, H."Kinase inhibitors fail to induce mesenchymal-epithelial transition in fibroblasts from fibrotic lung tissue Corrigendum in /10.3892/ijmm.2020.4660". International Journal of Molecular Medicine 32, no. 2 (2013): 430-438. https://doi.org/10.3892/ijmm.2013.1415
Copy and paste a formatted citation
x
Spandidos Publications style
Xu X, Wan X, Geng J, Li F, Wang C and Dai H: Kinase inhibitors fail to induce mesenchymal-epithelial transition in fibroblasts from fibrotic lung tissue Corrigendum in /10.3892/ijmm.2020.4660. Int J Mol Med 32: 430-438, 2013.
APA
Xu, X., Wan, X., Geng, J., Li, F., Wang, C., & Dai, H. (2013). Kinase inhibitors fail to induce mesenchymal-epithelial transition in fibroblasts from fibrotic lung tissue Corrigendum in /10.3892/ijmm.2020.4660. International Journal of Molecular Medicine, 32, 430-438. https://doi.org/10.3892/ijmm.2013.1415
MLA
Xu, X., Wan, X., Geng, J., Li, F., Wang, C., Dai, H."Kinase inhibitors fail to induce mesenchymal-epithelial transition in fibroblasts from fibrotic lung tissue Corrigendum in /10.3892/ijmm.2020.4660". International Journal of Molecular Medicine 32.2 (2013): 430-438.
Chicago
Xu, X., Wan, X., Geng, J., Li, F., Wang, C., Dai, H."Kinase inhibitors fail to induce mesenchymal-epithelial transition in fibroblasts from fibrotic lung tissue Corrigendum in /10.3892/ijmm.2020.4660". International Journal of Molecular Medicine 32, no. 2 (2013): 430-438. https://doi.org/10.3892/ijmm.2013.1415
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