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International Journal of Molecular Medicine
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September 2013 Volume 32 Issue 3

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Article

Proteomic analysis of endothelial progenitor cells exposed to oxidative stress

  • Authors:
    • Ying Liu
    • Jun Wei
    • Ming Chang
    • Zhiqiang Liu
    • Dawei Li
    • Shengying Hu
    • Linsen Hu
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, the Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China, Institute of Neurology, China Three Gorges University, Yichang, Hubei 443000, P.R. China, Department of Neurology, The First Hospital, Jilin University, Changchun, Jilin 130021, P.R. China, Cardiovascular Department, The First Hospital, Jilin University, Changchun, Jilin 130021, P.R. China
  • Pages: 607-614
    |
    Published online on: June 18, 2013
       https://doi.org/10.3892/ijmm.2013.1419
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Abstract

Endothelial progenitor cells (EPCs) repair vascular damage and participate in neovascularization. Accumulating evidence has demonstrated that EPCs have therapeutic potential in reactive oxygen species (ROS)-mediated vascular diseases. In this study, to investigate the effects of oxidative stress on EPCs, EPCs were treated with H2O2 at different final concentrations for 3 h. MTT assay, scratch-wound assay and Matrigel invasion assay revealed that cell proliferation, migration and tubule formation and function, respectively, were impaired under H2O2 stress in a concentration-dependent manner. To determine protein response to H2O2 stress, two-dimensional differential in-gel electrophoresis (2D-DIGE) combined with matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF/TOF) mass spectrometry were performed. The results revealed that triosephosphate isomerase and ADP-sugar pyrophosphatase were downregulated, while peroxiredoxin-2, thioredoxin-dependent peroxide reductase, mitochondrial (Prx‑3), peroxiredoxin-6, EGF-containing fibulin-like extracellular matrix protein 1, vimentin and Rab GDP dissociation inhibitor α were upregulated in the H2O2-treated EPCs. To further confirm the results from mass spectrometry, the expression pattern of Prx-3 in response to H2O2 stress was examined by western blot analysis. The data presented in this study provide novel insight into the defensive mechanisms of EPCs and the pathways of oxidative damage in an oxidative environment.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Wei J, Chang M, Liu Z, Li D, Hu S and Hu L: Proteomic analysis of endothelial progenitor cells exposed to oxidative stress . Int J Mol Med 32: 607-614, 2013.
APA
Liu, Y., Wei, J., Chang, M., Liu, Z., Li, D., Hu, S., & Hu, L. (2013). Proteomic analysis of endothelial progenitor cells exposed to oxidative stress . International Journal of Molecular Medicine, 32, 607-614. https://doi.org/10.3892/ijmm.2013.1419
MLA
Liu, Y., Wei, J., Chang, M., Liu, Z., Li, D., Hu, S., Hu, L."Proteomic analysis of endothelial progenitor cells exposed to oxidative stress ". International Journal of Molecular Medicine 32.3 (2013): 607-614.
Chicago
Liu, Y., Wei, J., Chang, M., Liu, Z., Li, D., Hu, S., Hu, L."Proteomic analysis of endothelial progenitor cells exposed to oxidative stress ". International Journal of Molecular Medicine 32, no. 3 (2013): 607-614. https://doi.org/10.3892/ijmm.2013.1419
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Wei J, Chang M, Liu Z, Li D, Hu S and Hu L: Proteomic analysis of endothelial progenitor cells exposed to oxidative stress . Int J Mol Med 32: 607-614, 2013.
APA
Liu, Y., Wei, J., Chang, M., Liu, Z., Li, D., Hu, S., & Hu, L. (2013). Proteomic analysis of endothelial progenitor cells exposed to oxidative stress . International Journal of Molecular Medicine, 32, 607-614. https://doi.org/10.3892/ijmm.2013.1419
MLA
Liu, Y., Wei, J., Chang, M., Liu, Z., Li, D., Hu, S., Hu, L."Proteomic analysis of endothelial progenitor cells exposed to oxidative stress ". International Journal of Molecular Medicine 32.3 (2013): 607-614.
Chicago
Liu, Y., Wei, J., Chang, M., Liu, Z., Li, D., Hu, S., Hu, L."Proteomic analysis of endothelial progenitor cells exposed to oxidative stress ". International Journal of Molecular Medicine 32, no. 3 (2013): 607-614. https://doi.org/10.3892/ijmm.2013.1419
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