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Effect of angiopoietin-like protein 4 on rat pulmonary microvascular endothelial cells exposed to LPS

  • Authors:
    • Yuxi Wang
    • Hailong Chen
    • Hailong Li
    • Jingwen Zhang
    • Yanyan Gao
  • View Affiliations / Copyright

    Affiliations: Dalian Medical University, Dalian, Liaoning, P.R. China, Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, P.R. China, Department of Central Laboratory, Dalian Municipal Central Hospital, Dalian, Liaoning, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 568-576
    |
    Published online on: June 20, 2013
       https://doi.org/10.3892/ijmm.2013.1420
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Abstract

Pulmonary microvascular endothelial cells (PMVECs) possess highly proliferative and angiogenic capacities and are localized at the critical interface between the blood and microvessel wall; they are the primary targets of in­flammatory cytokines during lung inflammation. Angiopoietin-like protein 4 (Angptl4) is a circulating protein that has recently been im­plicated in the regulation of angiogenesis and metastasis. This study aimed to investigate the effect of Angptl4 on rat PMVECs (RPMVECs) exposed to lipopolysaccharide (LPS). The cell culture was stimulated with LPS. Total Angptl4 cDNA was obtained from Source BioScience. The PCR product was cloned into the pcDNA3.1-eGFP or the pcDNA3.1‑eGFP‑Angptl4 vector, which were then transfec­ted into the RPMVECs using SuperFect transfection reagent. The Angptl4 mRNA levels, protein levels and cell morphology of the RPMVECs in the experimental groups were detected using real time‑PCR, western blot analysis, MTT assay, ELISA and confocal microscopy methods, respective­ly. The Angptl4 expression vector, pcDNA3.1‑eGFP-Angptl4, was successfully constructed. The Angptl4 mRNA level in the LPS-pcDNA3.1-eGFP-transfected group (blank control) was slightly increased and was significantly higher in the experimental group compared with the empty vector and blank control group with significant differences. Pro-apoptotic caspase-8, -9 and Bax protein were inhibited, while p-AKT/AKT and p-Mek1/2 protein expression was also decreased. The rosiglitazone group had significantly decreased levels of the inflammatory cytokine, tumor necrosis factor (TNF)-α (P<0.01). The overexpression of Angptl4 inhibited the LPS-induced increase in the per­meability of the RPMVECs, which was associated with the depolymerization of central F-actin in the RPMVECs. In conclusion, our study demonstrates that the overexpression of Angptl4 exerts protective, anti‑inflammatory and anti‑angioge­nic effects. It re­presents a novel therapeutic target gene for the treatment of acute lung injury induced by LPS.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Chen H, Li H, Zhang J and Gao Y: Effect of angiopoietin-like protein 4 on rat pulmonary microvascular endothelial cells exposed to LPS. Int J Mol Med 32: 568-576, 2013.
APA
Wang, Y., Chen, H., Li, H., Zhang, J., & Gao, Y. (2013). Effect of angiopoietin-like protein 4 on rat pulmonary microvascular endothelial cells exposed to LPS. International Journal of Molecular Medicine, 32, 568-576. https://doi.org/10.3892/ijmm.2013.1420
MLA
Wang, Y., Chen, H., Li, H., Zhang, J., Gao, Y."Effect of angiopoietin-like protein 4 on rat pulmonary microvascular endothelial cells exposed to LPS". International Journal of Molecular Medicine 32.3 (2013): 568-576.
Chicago
Wang, Y., Chen, H., Li, H., Zhang, J., Gao, Y."Effect of angiopoietin-like protein 4 on rat pulmonary microvascular endothelial cells exposed to LPS". International Journal of Molecular Medicine 32, no. 3 (2013): 568-576. https://doi.org/10.3892/ijmm.2013.1420
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Chen H, Li H, Zhang J and Gao Y: Effect of angiopoietin-like protein 4 on rat pulmonary microvascular endothelial cells exposed to LPS. Int J Mol Med 32: 568-576, 2013.
APA
Wang, Y., Chen, H., Li, H., Zhang, J., & Gao, Y. (2013). Effect of angiopoietin-like protein 4 on rat pulmonary microvascular endothelial cells exposed to LPS. International Journal of Molecular Medicine, 32, 568-576. https://doi.org/10.3892/ijmm.2013.1420
MLA
Wang, Y., Chen, H., Li, H., Zhang, J., Gao, Y."Effect of angiopoietin-like protein 4 on rat pulmonary microvascular endothelial cells exposed to LPS". International Journal of Molecular Medicine 32.3 (2013): 568-576.
Chicago
Wang, Y., Chen, H., Li, H., Zhang, J., Gao, Y."Effect of angiopoietin-like protein 4 on rat pulmonary microvascular endothelial cells exposed to LPS". International Journal of Molecular Medicine 32, no. 3 (2013): 568-576. https://doi.org/10.3892/ijmm.2013.1420
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