Induction of Hantaan virus-specific immune responses in C57BL/6 mice by immunization with a modified recombinant adenovirus containing the chimeric gene, GcS0.7

Li,Kai; Li,Pu-Yuan; Wu,Xing-An; Zhang,Liang; Liu,Zi-Yu; Yu,Lan; Zhang,Lei; Cheng,Lin-Feng; Bai,Wen-Tao; Zhang,Fang-Lin; Xu,Zhi-Kai

doi:10.3892/ijmm.2013.1421

September 2013 Volume 32 Issue 3

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September 2013 Volume 32 Issue 3

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Article

Induction of Hantaan virus-specific immune responses in C57BL/6 mice by immunization with a modified recombinant adenovirus containing the chimeric gene, GcS0.7

Authors:
- Kai Li
- Pu-Yuan Li
- Xing-An Wu
- Liang Zhang
- Zi-Yu Liu
- Lan Yu
- Lei Zhang
- Lin-Feng Cheng
- Wen-Tao Bai
- Fang-Lin Zhang
- Zhi-Kai Xu
View Affiliations / Copyright

Affiliations: Department of Microbiology, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Minimally Invasive Surgery of the Military General Surgery Center, General Hospital of People's Liberation Army Chengdu Military Region, Chengdu, Sichuan 610083, P.R. China
Pages: 709-716
|
Published online on: June 20, 2013

https://doi.org/10.3892/ijmm.2013.1421
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Abstract

Hantavirus glycoprotein Gc is one of the main components that contribute to the generation of humoral immune responses, while the nucleocapsid protein (NP) is involved in cellular immune responses through the induction of antibody-dependent cytotoxic T cells. In this study, a chimeric gene, GcS0.7, which encodes a fusion protein containing Gc and truncated NP, was constructed as a candidate for Hantaan virus (HTNV) vaccine development. The chimeric gene was cloned into an adenoviral vector in conjunction with the powerful hybrid cytomegalovirus (CMV) enhancer/chicken β-actin (CAG) promoter or the woodchuck hepatitis virus (WHV) post-transcriptional regulatory element (WPRE), or both. Both elements increased the expression level of the fusion protein. The rAd-GcS0.7-pCAG group demonstrated the highest fusion protein expression level, with a 2.3‑fold increase compared with the unmodified adenoviral vector. To further evaluate the humoral and cellular immunity induced by the recombinant adenovirus, the antibody titers, interferon (IFN)-γ secretion level and cytotoxic T cell ratio were detected in immunized mice. The strongest HTNV‑specific humoral and cellular immune responses were detected in the rAd-GcS0.7‑pCAG group. The immunogenicity of these recombinant adenoviruses was compared with that of the inactivated vaccine through a series of immunological assays. In terms of the cellular immune responses, the rAd-GcS0.7-pCAG group even exceeded those induced by the vaccine control. The CAG hybrid promoter improved not only the expression level, but also the immunogenicity of the fusion protein, and may thus provide a promising strategy for HTNV vaccine research.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Induction of Hantaan virus-specific immune responses in C57BL/6 mice by immunization with a modified recombinant adenovirus containing the chimeric gene, GcS0.7

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