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Serum microRNA expression levels can predict lymph node metastasis in patients with early-stage cervical squamous cell carcinoma

  • Authors:
    • Junying Chen
    • Desheng Yao
    • Yue Li
    • Hong Chen
    • Chanjuan He
    • Nan Ding
    • Yan Lu
    • Tingyu Ou
    • Shan Zhao
    • Li Li
    • Fengyi Long
  • View Affiliations / Copyright

    Affiliations: Department of Gynecological Oncology, Affiliated Cancer Hospital of Guangxi Medical University, Nanning, Guangxi 530022, P.R. China, Department of Health and Statistics, School of Public Health, Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Gynecology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530022, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 557-567
    |
    Published online on: June 21, 2013
       https://doi.org/10.3892/ijmm.2013.1424
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Abstract

Circulating microRNA expression levels can serve as diagnostic/prognostic biomarkers in several types of malignant tumors; however, to our knowledge, there have been reports describing their value in cervical squamous cell carcinoma (SCC). In this study, we used hybridization arrays to compare the microRNA expression profiles in cervical squamous cell carcinomas (SCC) samples among patients with lymph node metastasis (LNM) or without LNM; 89 microRNAs were found to fit our inclusion criteria. Using quantitative PCR (qPCR), we examined the expression levels of these microRNAs in cervical cancer tissue, as well as in serum from patients and healthy women. We compared the expression levels between patients with LNM (n=40) and those without LNM (n=40) and healthy controls (n=20). Using regression analysis, we generated a comprehensive set of marker microRNAs and drew the fitted binormal receiver operating characteristic (ROC) curves to access the predictive value. We identified 6 serum microRNAs that can predict LNM in cervical SCC patients; these microRNAs were miR-1246, miR-20a, miR-2392, miR-3147, miR-3162-5p and miR-4484. The area under the curve (AUC) of the comprehensive set of serum microRNAs predicting LNM was 0.932 (sensitivity, 0.856; specificity, 0.850). The predictive value of the serum microRNAs was inferior to that in tissue (AUC 0.992; sensitivity, 0.967; specificity, 0.950; P=0.018). We compared the LNM predictive value of serum microRNAs and SCC antigen (SCC-Ag) by drawing fitted binormal ROC curves However, serum microRNA analysis is by far superior to serum SCC‑Ag analysis (AUC 0.713; sensitivity, 0.612; specificity, 0.700; P<0.0001). Serum microRNAs are a good predictor of LNM with clinical value in early-stage cervical SCC.
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Copy and paste a formatted citation
Spandidos Publications style
Chen J, Yao D, Li Y, Chen H, He C, Ding N, Lu Y, Ou T, Zhao S, Li L, Li L, et al: Serum microRNA expression levels can predict lymph node metastasis in patients with early-stage cervical squamous cell carcinoma. Int J Mol Med 32: 557-567, 2013.
APA
Chen, J., Yao, D., Li, Y., Chen, H., He, C., Ding, N. ... Long, F. (2013). Serum microRNA expression levels can predict lymph node metastasis in patients with early-stage cervical squamous cell carcinoma. International Journal of Molecular Medicine, 32, 557-567. https://doi.org/10.3892/ijmm.2013.1424
MLA
Chen, J., Yao, D., Li, Y., Chen, H., He, C., Ding, N., Lu, Y., Ou, T., Zhao, S., Li, L., Long, F."Serum microRNA expression levels can predict lymph node metastasis in patients with early-stage cervical squamous cell carcinoma". International Journal of Molecular Medicine 32.3 (2013): 557-567.
Chicago
Chen, J., Yao, D., Li, Y., Chen, H., He, C., Ding, N., Lu, Y., Ou, T., Zhao, S., Li, L., Long, F."Serum microRNA expression levels can predict lymph node metastasis in patients with early-stage cervical squamous cell carcinoma". International Journal of Molecular Medicine 32, no. 3 (2013): 557-567. https://doi.org/10.3892/ijmm.2013.1424
Copy and paste a formatted citation
x
Spandidos Publications style
Chen J, Yao D, Li Y, Chen H, He C, Ding N, Lu Y, Ou T, Zhao S, Li L, Li L, et al: Serum microRNA expression levels can predict lymph node metastasis in patients with early-stage cervical squamous cell carcinoma. Int J Mol Med 32: 557-567, 2013.
APA
Chen, J., Yao, D., Li, Y., Chen, H., He, C., Ding, N. ... Long, F. (2013). Serum microRNA expression levels can predict lymph node metastasis in patients with early-stage cervical squamous cell carcinoma. International Journal of Molecular Medicine, 32, 557-567. https://doi.org/10.3892/ijmm.2013.1424
MLA
Chen, J., Yao, D., Li, Y., Chen, H., He, C., Ding, N., Lu, Y., Ou, T., Zhao, S., Li, L., Long, F."Serum microRNA expression levels can predict lymph node metastasis in patients with early-stage cervical squamous cell carcinoma". International Journal of Molecular Medicine 32.3 (2013): 557-567.
Chicago
Chen, J., Yao, D., Li, Y., Chen, H., He, C., Ding, N., Lu, Y., Ou, T., Zhao, S., Li, L., Long, F."Serum microRNA expression levels can predict lymph node metastasis in patients with early-stage cervical squamous cell carcinoma". International Journal of Molecular Medicine 32, no. 3 (2013): 557-567. https://doi.org/10.3892/ijmm.2013.1424
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