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International Journal of Molecular Medicine
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September 2013 Volume 32 Issue 3

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Article

Association between mRNA levels of DNMT1, DNMT3A, DNMT3B, MBD2 and LINE-1 methylation status in infants with tetralogy of Fallot

  • Authors:
    • Wei Sheng
    • Yanyan Qian
    • Huijun Wang
    • Xiaojing Ma
    • Ping Zhang
    • Long Chen
    • Duan Ma
    • Guoying Huang
  • View Affiliations / Copyright

    Affiliations: Children's Hospital, Fudan University, Shanghai 201102, P.R. China, Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai Medical College, Fudan University, Shanghai 200032, P.R. China, Department of Forensic Medicine, Fudan University, Shanghai 200032, P.R. China
  • Pages: 694-702
    |
    Published online on: July 2, 2013
       https://doi.org/10.3892/ijmm.2013.1427
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Abstract

DNA methylation is catalyzed and maintained by DNA methyltransferases (DNMTs: DNMT1, DNMT3A and DNMT3B) and methyl-CpG-binding domain protein 2 (MBD2). However, little is known about the biological and clinical significance of the expression changes of DNMTs and MBD2 and their association with the methylation levels of long interspersed nuclear element-1 (LINE-1) in patients with tetralogy of Fallot (TOF). In this study, quantitative RT-PCR (qRT-PCR) was applied to analyze the mRNA levels of DNMTs and MBD2. The methylation status of LINE-1 was measured using the sequenom MassARRAY platform. The mRNA levels of the DNMTs and MBD2 showed a statistically significant decrease in the patients with TOF (P<0.001). The results also showed that patients with TOF had significantly lower global DNA methylation levels with a median of 61.50% [interquartile range (IQR), 59.78-63.77] compared with 63.54% (IQR, 62.49‑64.88) among the controls (P=0.0099). In the controls, only DNMT1 showed a significant positive correlation with the DNMT3A mRNA levels (r=0.718, P=0.002). Of note, the DNMT1, DNMT3A, DNMT3B and MBD2 mRNA levels positively correlated with each other; this was statistically significant (P<0.05). A significant positive correlation with the global DNA methylation status was observed only for MBD2 (r=-0.579, P=0.005) in patients with TOF. In conclusion, lower LINE-1 methylation levels significantly correlate with aberrant MBD2 mRNA levels. The lower expression of DNMT1 and DNMT3B may play an important role in the pathogenesis of TOF.
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Copy and paste a formatted citation
Spandidos Publications style
Sheng W, Qian Y, Wang H, Ma X, Zhang P, Chen L, Ma D and Huang G: Association between mRNA levels of DNMT1, DNMT3A, DNMT3B, MBD2 and LINE-1 methylation status in infants with tetralogy of Fallot. Int J Mol Med 32: 694-702, 2013.
APA
Sheng, W., Qian, Y., Wang, H., Ma, X., Zhang, P., Chen, L. ... Huang, G. (2013). Association between mRNA levels of DNMT1, DNMT3A, DNMT3B, MBD2 and LINE-1 methylation status in infants with tetralogy of Fallot. International Journal of Molecular Medicine, 32, 694-702. https://doi.org/10.3892/ijmm.2013.1427
MLA
Sheng, W., Qian, Y., Wang, H., Ma, X., Zhang, P., Chen, L., Ma, D., Huang, G."Association between mRNA levels of DNMT1, DNMT3A, DNMT3B, MBD2 and LINE-1 methylation status in infants with tetralogy of Fallot". International Journal of Molecular Medicine 32.3 (2013): 694-702.
Chicago
Sheng, W., Qian, Y., Wang, H., Ma, X., Zhang, P., Chen, L., Ma, D., Huang, G."Association between mRNA levels of DNMT1, DNMT3A, DNMT3B, MBD2 and LINE-1 methylation status in infants with tetralogy of Fallot". International Journal of Molecular Medicine 32, no. 3 (2013): 694-702. https://doi.org/10.3892/ijmm.2013.1427
Copy and paste a formatted citation
x
Spandidos Publications style
Sheng W, Qian Y, Wang H, Ma X, Zhang P, Chen L, Ma D and Huang G: Association between mRNA levels of DNMT1, DNMT3A, DNMT3B, MBD2 and LINE-1 methylation status in infants with tetralogy of Fallot. Int J Mol Med 32: 694-702, 2013.
APA
Sheng, W., Qian, Y., Wang, H., Ma, X., Zhang, P., Chen, L. ... Huang, G. (2013). Association between mRNA levels of DNMT1, DNMT3A, DNMT3B, MBD2 and LINE-1 methylation status in infants with tetralogy of Fallot. International Journal of Molecular Medicine, 32, 694-702. https://doi.org/10.3892/ijmm.2013.1427
MLA
Sheng, W., Qian, Y., Wang, H., Ma, X., Zhang, P., Chen, L., Ma, D., Huang, G."Association between mRNA levels of DNMT1, DNMT3A, DNMT3B, MBD2 and LINE-1 methylation status in infants with tetralogy of Fallot". International Journal of Molecular Medicine 32.3 (2013): 694-702.
Chicago
Sheng, W., Qian, Y., Wang, H., Ma, X., Zhang, P., Chen, L., Ma, D., Huang, G."Association between mRNA levels of DNMT1, DNMT3A, DNMT3B, MBD2 and LINE-1 methylation status in infants with tetralogy of Fallot". International Journal of Molecular Medicine 32, no. 3 (2013): 694-702. https://doi.org/10.3892/ijmm.2013.1427
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