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October 2013 Volume 32 Issue 4

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Article

Ethanol extract of Cnidium officinale exhibits anti-inflammatory effects in BV2 microglial cells by suppressing NF-κB nuclear translocation and the activation of the PI3K/Akt signaling pathway

  • Authors:
    • Shin Hwa Lee
    • Jun Hyuk Lee
    • Eun Young Oh
    • Gi-Young Kim
    • Byung Tae Choi
    • Cheolmin Kim
    • Yung Hyun Choi
  • View Affiliations / Copyright

    Affiliations: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea, Biotechnology Examination Division, Chemistry and Biotechnology Examination Bureau, Korean Intellectual Property Office, Daejeon 302-701, Republic of Korea, Division of Meridian and Structural Medicine, School of Korean Medicine, Busan National University, Yangsan 626-870, Republic of Korea, Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea, Research Center for Anti-Aging Technology Development, Busan National University, Busan 609-735, Republic of Korea, Department of Biochemistry, Dongeui University College of Oriental Medicine, Busan 614-052, Republic of Korea
  • Pages: 876-882
    |
    Published online on: July 16, 2013
       https://doi.org/10.3892/ijmm.2013.1447
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Abstract

Chronic microglial activation endangers neuronal survival through the release of various toxic pro-inflammatory molecules; thus, negative regulators of microglial activation have been identified as potential therapeutic candidates for several neurological diseases. In this study, we investigated the inhibitory effects of an ethanol extract of Cnidium officinale rhizomes (EECO), which has been used as a herbal drug in Oriental medicine, on the production of lipopolysaccharide (LPS)-induced pro-inflammatory mediators, such as nitric oxide (NO) and prostaglandin E2 (PGE2), as well as that of pro-inflammatory cytokines in BV2 microglia cells. EECO significantly inhibited the excess production of NO and PGE2 in LPS-stimulated BV2 microglia cells. It also attenuated the expression of inducible NO synthase, cyclooxygenase-2, as well as that of pro-inflammatory cytokines, such as interleukin-1β and tumor necrosis factor-α. Moreover, EECO exhibited anti-inflammatory properties by suppressing nuclear factor-κB (NF-κB) translocation and the activation of the phosphoinositide 3-kinase/Akt pathway in LPS-stimulated BV2 cells. These results indicate that EECO exerts anti-inflammatory effects in LPS-stimulated BV2 microglial cells by inhibiting pro-inflammatory mediators and cytokine production by blocking the NF-κB pathway. These findings suggest that EECO has substantial therapeutic potential for the treatment of neurodegenerative diseases accompanied by microglial activation.
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Copy and paste a formatted citation
Spandidos Publications style
Lee SH, Lee JH, Oh EY, Kim G, Choi BT, Kim C and Choi YH: Ethanol extract of Cnidium officinale exhibits anti-inflammatory effects in BV2 microglial cells by suppressing NF-κB nuclear translocation and the activation of the PI3K/Akt signaling pathway. Int J Mol Med 32: 876-882, 2013.
APA
Lee, S.H., Lee, J.H., Oh, E.Y., Kim, G., Choi, B.T., Kim, C., & Choi, Y.H. (2013). Ethanol extract of Cnidium officinale exhibits anti-inflammatory effects in BV2 microglial cells by suppressing NF-κB nuclear translocation and the activation of the PI3K/Akt signaling pathway. International Journal of Molecular Medicine, 32, 876-882. https://doi.org/10.3892/ijmm.2013.1447
MLA
Lee, S. H., Lee, J. H., Oh, E. Y., Kim, G., Choi, B. T., Kim, C., Choi, Y. H."Ethanol extract of Cnidium officinale exhibits anti-inflammatory effects in BV2 microglial cells by suppressing NF-κB nuclear translocation and the activation of the PI3K/Akt signaling pathway". International Journal of Molecular Medicine 32.4 (2013): 876-882.
Chicago
Lee, S. H., Lee, J. H., Oh, E. Y., Kim, G., Choi, B. T., Kim, C., Choi, Y. H."Ethanol extract of Cnidium officinale exhibits anti-inflammatory effects in BV2 microglial cells by suppressing NF-κB nuclear translocation and the activation of the PI3K/Akt signaling pathway". International Journal of Molecular Medicine 32, no. 4 (2013): 876-882. https://doi.org/10.3892/ijmm.2013.1447
Copy and paste a formatted citation
x
Spandidos Publications style
Lee SH, Lee JH, Oh EY, Kim G, Choi BT, Kim C and Choi YH: Ethanol extract of Cnidium officinale exhibits anti-inflammatory effects in BV2 microglial cells by suppressing NF-κB nuclear translocation and the activation of the PI3K/Akt signaling pathway. Int J Mol Med 32: 876-882, 2013.
APA
Lee, S.H., Lee, J.H., Oh, E.Y., Kim, G., Choi, B.T., Kim, C., & Choi, Y.H. (2013). Ethanol extract of Cnidium officinale exhibits anti-inflammatory effects in BV2 microglial cells by suppressing NF-κB nuclear translocation and the activation of the PI3K/Akt signaling pathway. International Journal of Molecular Medicine, 32, 876-882. https://doi.org/10.3892/ijmm.2013.1447
MLA
Lee, S. H., Lee, J. H., Oh, E. Y., Kim, G., Choi, B. T., Kim, C., Choi, Y. H."Ethanol extract of Cnidium officinale exhibits anti-inflammatory effects in BV2 microglial cells by suppressing NF-κB nuclear translocation and the activation of the PI3K/Akt signaling pathway". International Journal of Molecular Medicine 32.4 (2013): 876-882.
Chicago
Lee, S. H., Lee, J. H., Oh, E. Y., Kim, G., Choi, B. T., Kim, C., Choi, Y. H."Ethanol extract of Cnidium officinale exhibits anti-inflammatory effects in BV2 microglial cells by suppressing NF-κB nuclear translocation and the activation of the PI3K/Akt signaling pathway". International Journal of Molecular Medicine 32, no. 4 (2013): 876-882. https://doi.org/10.3892/ijmm.2013.1447
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