A method for the ultrastructural preservation of tiny percutaneous needle biopsy material from skeletal muscle

Pietrangelo,Tiziana; Perni,Stefano; Di Tano,Guglielmo; Fanò-Illic,Giorgio; Franzini-Armstrong,Clara

doi:10.3892/ijmm.2013.1454

October 2013 Volume 32 Issue 4

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October 2013 Volume 32 Issue 4

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Article

A method for the ultrastructural preservation of tiny percutaneous needle biopsy material from skeletal muscle

Authors:
- Tiziana Pietrangelo
- Stefano Perni
- Guglielmo Di Tano
- Giorgio Fanò-Illic
- Clara Franzini-Armstrong
View Affiliations / Copyright

Affiliations: Department of Neuroscience and Imaging, Section of Physiology and Physiopathology, ‘G. d’Annunzio’ University of Chieti-Pescara and Clinical Research Center (CRC) of G. d'Annunzio Foundation, Chieti I-66100, Italy, Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
Pages: 965-970
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Published online on: July 23, 2013

https://doi.org/10.3892/ijmm.2013.1454
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Abstract

Skeletal muscle biopsies require transecting the muscle fibers resulting, in structural damage near the cut ends. Classically, the optimal ultrastructural preservation has been obtained by the use of relatively large biopsies in which the tissue fibers are restrained by ligating to a suitable retaining support prior to excision, and by examining regions at some distance from the cut ends. However, these methods require invasive surgical procedures. In the present study, we present and substantiate an alternative approach that allows for the excellent ultrastructural preservation of needle biopsy samples, even the very small samples obtained through tiny percutaneous needle biopsy (TPNB). TPNB represents an advantage, relative to standard muscle biopsy techniques and to other needle biopsies currently in use, as in addition to not requiring a skin incision, it leaves no scars in the muscle and requires an extremely brief recovery period. It is most appropriate for obtaining repeated samples in horizontal studies, e.g., in order to follow changes with athletic training and/or aging in a single individual and for studies of sarcopenic muscles in elderly patients. Due to the small size of the sample, TPNB may present limited usefulness for classical pathology diagnostics. However, it offers the major advantage of allowing multiple samples within a single session and this may be useful under specific circumstances.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

A method for the ultrastructural preservation of tiny percutaneous needle biopsy material from skeletal muscle

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