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Article

Decoy receptor 3 regulates the expression of various genes in rheumatoid arthritis synovial fibroblasts

  • Authors:
    • Koji Fukuda
    • Yasushi Miura
    • Toshihisa Maeda
    • Masayasu Takahashi
    • Shinya Hayashi
    • Masahiro Kurosaka
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Chuo, Kobe, Hyogo 650-0017, Japan, Division of Orthopaedic Science, Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Suma, Kobe, Hyogo 654-0142, Japan, Department of Orthopaedic Surgery, Kohnan Hospital, Higashinada, Kobe, Hyogo 658-0064, Japan
  • Pages: 910-916
    |
    Published online on: August 1, 2013
       https://doi.org/10.3892/ijmm.2013.1461
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Abstract

Decoy receptor 3 (DcR3), a member of the tumor necrosis factor (TNF) receptor (TNFR) superfamily, lacks the transmembrane domain of conventional TNFRs in order to be a secreted protein. DcR3 competitively binds and inhibits members of the TNF family, including Fas ligand (FasL), LIGHT and TNF-like ligand 1A (TL1A). We previously reported that TNFα-induced DcR3 overexpression in rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) protects cells from Fas-induced apoptosis. Previous studies have suggested that DcR3 acting as a ligand directly induces the differentiation of macrophages into osteoclasts. Furthermore, we reported that DcR3 induces very late antigen-4 (VLA-­4) expression in THP-1 macrophages, inhibiting cycloheximide-induced apoptosis and that DcR3 binds to membrane-bound TL1A expressed on RA-FLS, resulting in the negative regulation of cell proliferation induced by inflammatory cytokines. In the current study, we used cDNA microarray to search for genes in RA-FLS whose expression was regulated by the ligation of DcR3. The experiments revealed the expression profiles of genes in RA-FLS regulated by DcR3. The profiles showed that among the 100 genes most significantly regulated by DcR3, 45 were upregulated and 55 were downregulated. The upregulated genes were associated with protein complex assembly, cell motility, regulation of transcription, cellular protein catabolic processes, cell membrane, nucleotide binding and glycosylation. The downregulated genes were associated with transcription regulator activity, RNA biosynthetic processes, cytoskeleton, zinc finger region, protein complex assembly, phosphate metabolic processes, mitochondrion, ion transport, nucleotide binding and cell fractionation. Further study of the genes detected in the current study may provide insight into the pathogenesis and treatment of rheumatoid arthritis by DcR3-TL1A signaling.
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Copy and paste a formatted citation
Spandidos Publications style
Fukuda K, Miura Y, Maeda T, Takahashi M, Hayashi S and Kurosaka M: Decoy receptor 3 regulates the expression of various genes in rheumatoid arthritis synovial fibroblasts. Int J Mol Med 32: 910-916, 2013.
APA
Fukuda, K., Miura, Y., Maeda, T., Takahashi, M., Hayashi, S., & Kurosaka, M. (2013). Decoy receptor 3 regulates the expression of various genes in rheumatoid arthritis synovial fibroblasts. International Journal of Molecular Medicine, 32, 910-916. https://doi.org/10.3892/ijmm.2013.1461
MLA
Fukuda, K., Miura, Y., Maeda, T., Takahashi, M., Hayashi, S., Kurosaka, M."Decoy receptor 3 regulates the expression of various genes in rheumatoid arthritis synovial fibroblasts". International Journal of Molecular Medicine 32.4 (2013): 910-916.
Chicago
Fukuda, K., Miura, Y., Maeda, T., Takahashi, M., Hayashi, S., Kurosaka, M."Decoy receptor 3 regulates the expression of various genes in rheumatoid arthritis synovial fibroblasts". International Journal of Molecular Medicine 32, no. 4 (2013): 910-916. https://doi.org/10.3892/ijmm.2013.1461
Copy and paste a formatted citation
x
Spandidos Publications style
Fukuda K, Miura Y, Maeda T, Takahashi M, Hayashi S and Kurosaka M: Decoy receptor 3 regulates the expression of various genes in rheumatoid arthritis synovial fibroblasts. Int J Mol Med 32: 910-916, 2013.
APA
Fukuda, K., Miura, Y., Maeda, T., Takahashi, M., Hayashi, S., & Kurosaka, M. (2013). Decoy receptor 3 regulates the expression of various genes in rheumatoid arthritis synovial fibroblasts. International Journal of Molecular Medicine, 32, 910-916. https://doi.org/10.3892/ijmm.2013.1461
MLA
Fukuda, K., Miura, Y., Maeda, T., Takahashi, M., Hayashi, S., Kurosaka, M."Decoy receptor 3 regulates the expression of various genes in rheumatoid arthritis synovial fibroblasts". International Journal of Molecular Medicine 32.4 (2013): 910-916.
Chicago
Fukuda, K., Miura, Y., Maeda, T., Takahashi, M., Hayashi, S., Kurosaka, M."Decoy receptor 3 regulates the expression of various genes in rheumatoid arthritis synovial fibroblasts". International Journal of Molecular Medicine 32, no. 4 (2013): 910-916. https://doi.org/10.3892/ijmm.2013.1461
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