Development of a dendritic cell vaccine encoding multiple cytotoxic T lymphocyte epitopes targeting hepatitis C virus

  • Authors:
    • Yun Zhou
    • Futao Zhao
    • Lin Chen
    • Li Ma
    • Yu Wang
    • Yu He
    • Zhiyuan Ma
    • Haili Liu
    • Yonghong Guo
    • Ying Zhang
    • Zhiqiang Yao
    • Chunqiu Hao
    • Zhansheng Jia
  • View Affiliations

  • Published online on: August 7, 2013     https://doi.org/10.3892/ijmm.2013.1466
  • Pages: 901-909
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Abstract

The aim of the present study was to develop a dendritic cell (DC) vaccine encoding hepatitis C virus (HCV) multiple cytotoxic T lymphocyte (CTL) epitopes that can stimulate T cell responses in vitro, and can be used for immunization in vivo. DCs were infected with recombinant replication-defective adenoviruses (Ads) expressing 2 HCV sequences fused with green fluorescent protein (GFP) and FLAG tags. One sequence (sequence 1) contained the HCV CTL epitopes, NS4B 1793‑1801 and P7 774-782, as well as the HCV Th epitope, NS3 1248‑1261. A second sequence (sequence 2) was the positive epitope control which contained HCV core 35-44, core 132‑140 and NS3 1248-1261. The efficiency of infection was detected by flow cytometry and the expression of HCV epitopes in the DCs was confirmed by RT-PCR and western blot analysis. Ad infection significantly enhanced DC maturation and interleukin (IL)-12p70 production, resulting in T cell proliferation and increased interferon-γ secretion. The CTLs stimulated by Ad-infected DCs specifically killed Huh7.5 human hepatoma cells. The recombinant Ad-expressing multiple CTL HCV epitopes effectively infected the DCs in vitro and promoted T cell antiviral immune responses, thereby laying the foundation for the development of anti-HCV DC vaccines.
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October 2013
Volume 32 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhou Y, Zhao F, Chen L, Ma L, Wang Y, He Y, Ma Z, Liu H, Guo Y, Zhang Y, Zhang Y, et al: Development of a dendritic cell vaccine encoding multiple cytotoxic T lymphocyte epitopes targeting hepatitis C virus. Int J Mol Med 32: 901-909, 2013
APA
Zhou, Y., Zhao, F., Chen, L., Ma, L., Wang, Y., He, Y. ... Jia, Z. (2013). Development of a dendritic cell vaccine encoding multiple cytotoxic T lymphocyte epitopes targeting hepatitis C virus. International Journal of Molecular Medicine, 32, 901-909. https://doi.org/10.3892/ijmm.2013.1466
MLA
Zhou, Y., Zhao, F., Chen, L., Ma, L., Wang, Y., He, Y., Ma, Z., Liu, H., Guo, Y., Zhang, Y., Yao, Z., Hao, C., Jia, Z."Development of a dendritic cell vaccine encoding multiple cytotoxic T lymphocyte epitopes targeting hepatitis C virus". International Journal of Molecular Medicine 32.4 (2013): 901-909.
Chicago
Zhou, Y., Zhao, F., Chen, L., Ma, L., Wang, Y., He, Y., Ma, Z., Liu, H., Guo, Y., Zhang, Y., Yao, Z., Hao, C., Jia, Z."Development of a dendritic cell vaccine encoding multiple cytotoxic T lymphocyte epitopes targeting hepatitis C virus". International Journal of Molecular Medicine 32, no. 4 (2013): 901-909. https://doi.org/10.3892/ijmm.2013.1466