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Article

Development of a dendritic cell vaccine encoding multiple cytotoxic T lymphocyte epitopes targeting hepatitis C virus

  • Authors:
    • Yun Zhou
    • Futao Zhao
    • Lin Chen
    • Li Ma
    • Yu Wang
    • Yu He
    • Zhiyuan Ma
    • Haili Liu
    • Yonghong Guo
    • Ying Zhang
    • Zhiqiang Yao
    • Chunqiu Hao
    • Zhansheng Jia
  • View Affiliations / Copyright

    Affiliations: Center of Diagnosis and Treatment for Infectious Diseases of Chinese PLA, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China, Department of Medical Quality Control, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China, Department of Internal Medicine, Division of Infectious Diseases, East Tennessee State University Quillen College of Medicine, Johnson City, TN 37614, USA
  • Pages: 901-909
    |
    Published online on: August 7, 2013
       https://doi.org/10.3892/ijmm.2013.1466
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Abstract

The aim of the present study was to develop a dendritic cell (DC) vaccine encoding hepatitis C virus (HCV) multiple cytotoxic T lymphocyte (CTL) epitopes that can stimulate T cell responses in vitro, and can be used for immunization in vivo. DCs were infected with recombinant replication-defective adenoviruses (Ads) expressing 2 HCV sequences fused with green fluorescent protein (GFP) and FLAG tags. One sequence (sequence 1) contained the HCV CTL epitopes, NS4B 1793‑1801 and P7 774-782, as well as the HCV Th epitope, NS3 1248‑1261. A second sequence (sequence 2) was the positive epitope control which contained HCV core 35-44, core 132‑140 and NS3 1248-1261. The efficiency of infection was detected by flow cytometry and the expression of HCV epitopes in the DCs was confirmed by RT-PCR and western blot analysis. Ad infection significantly enhanced DC maturation and interleukin (IL)-12p70 production, resulting in T cell proliferation and increased interferon-γ secretion. The CTLs stimulated by Ad-infected DCs specifically killed Huh7.5 human hepatoma cells. The recombinant Ad-expressing multiple CTL HCV epitopes effectively infected the DCs in vitro and promoted T cell antiviral immune responses, thereby laying the foundation for the development of anti-HCV DC vaccines.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou Y, Zhao F, Chen L, Ma L, Wang Y, He Y, Ma Z, Liu H, Guo Y, Zhang Y, Zhang Y, et al: Development of a dendritic cell vaccine encoding multiple cytotoxic T lymphocyte epitopes targeting hepatitis C virus. Int J Mol Med 32: 901-909, 2013.
APA
Zhou, Y., Zhao, F., Chen, L., Ma, L., Wang, Y., He, Y. ... Jia, Z. (2013). Development of a dendritic cell vaccine encoding multiple cytotoxic T lymphocyte epitopes targeting hepatitis C virus. International Journal of Molecular Medicine, 32, 901-909. https://doi.org/10.3892/ijmm.2013.1466
MLA
Zhou, Y., Zhao, F., Chen, L., Ma, L., Wang, Y., He, Y., Ma, Z., Liu, H., Guo, Y., Zhang, Y., Yao, Z., Hao, C., Jia, Z."Development of a dendritic cell vaccine encoding multiple cytotoxic T lymphocyte epitopes targeting hepatitis C virus". International Journal of Molecular Medicine 32.4 (2013): 901-909.
Chicago
Zhou, Y., Zhao, F., Chen, L., Ma, L., Wang, Y., He, Y., Ma, Z., Liu, H., Guo, Y., Zhang, Y., Yao, Z., Hao, C., Jia, Z."Development of a dendritic cell vaccine encoding multiple cytotoxic T lymphocyte epitopes targeting hepatitis C virus". International Journal of Molecular Medicine 32, no. 4 (2013): 901-909. https://doi.org/10.3892/ijmm.2013.1466
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou Y, Zhao F, Chen L, Ma L, Wang Y, He Y, Ma Z, Liu H, Guo Y, Zhang Y, Zhang Y, et al: Development of a dendritic cell vaccine encoding multiple cytotoxic T lymphocyte epitopes targeting hepatitis C virus. Int J Mol Med 32: 901-909, 2013.
APA
Zhou, Y., Zhao, F., Chen, L., Ma, L., Wang, Y., He, Y. ... Jia, Z. (2013). Development of a dendritic cell vaccine encoding multiple cytotoxic T lymphocyte epitopes targeting hepatitis C virus. International Journal of Molecular Medicine, 32, 901-909. https://doi.org/10.3892/ijmm.2013.1466
MLA
Zhou, Y., Zhao, F., Chen, L., Ma, L., Wang, Y., He, Y., Ma, Z., Liu, H., Guo, Y., Zhang, Y., Yao, Z., Hao, C., Jia, Z."Development of a dendritic cell vaccine encoding multiple cytotoxic T lymphocyte epitopes targeting hepatitis C virus". International Journal of Molecular Medicine 32.4 (2013): 901-909.
Chicago
Zhou, Y., Zhao, F., Chen, L., Ma, L., Wang, Y., He, Y., Ma, Z., Liu, H., Guo, Y., Zhang, Y., Yao, Z., Hao, C., Jia, Z."Development of a dendritic cell vaccine encoding multiple cytotoxic T lymphocyte epitopes targeting hepatitis C virus". International Journal of Molecular Medicine 32, no. 4 (2013): 901-909. https://doi.org/10.3892/ijmm.2013.1466
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