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Article

Purpurogallin exerts anti‑inflammatory effects in lipopolysaccharide‑stimulated BV2 microglial cells through the inactivation of the NF‑κB and MAPK signaling pathways

  • Authors:
    • Hye Young Park
    • Tae Hoon Kim
    • Chan Gil Kim
    • Gi‑Young Kim
    • Cheol Min Kim
    • Nam Deuk Kim
    • Byung Woo Kim
    • Hye Jin Hwang
    • Yung Hyun Choi
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Busan National University, Busan 609‑735, Republic of Korea, Department of Herbal Medicinal Pharmacology, Daegu Haany University, Gyeongsan 712‑715, Republic of Korea, Department of Biotechnology, Konkuk University, Chungju 380‑701, Republic of Korea, Department of Marine Life Sciences, Jeju National University, Jeju 690‑756, Republic of Korea, Research Center for Anti‑Aging Technology Development, Busan National University, Busan 609‑735, Republic of Korea, Anti‑Aging Research Center and Blue‑Bio Industry RIC, Dongeui University, Busan 614‑714, Republic of Korea
  • Pages: 1171-1178
    |
    Published online on: August 30, 2013
       https://doi.org/10.3892/ijmm.2013.1478
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Abstract

In this study, we investigated the inhibitory effects of purpurogallin, a naturally occurring phenol, on the production of lipopolysaccharide (LPS)‑induced pro‑inflammatory mediators and cytokines in BV2 microglia cells. The cells were pre-treated or not with various concentrations of purpurogallin and then stimulated with 0.5 µg/ml LPS. Cell viability was measured by MTT assay. We also measured the production of nictric oxice (NO) and prostaglandin E2 (PGE2). Our data indicated that treatment with purpurogallin significantly inhibited the excessive production of NO and PGE2 in LPS‑stimulated BV2 microglial cells. These inhibitory effects were associated with the downregulation of key enzymes for NO and PGE2, inducible NO synthase (iNOS) and cyclooxygenase‑2 (COX2) expression, respectively. Purpurogallin also attenuated the production of pro‑inflammatory cytokines, including interleukin‑1β (IL‑1β) and tumor necrosis factor‑α (TNF‑α) by suppressing their mRNA and protein expression. The molecular mechanisms underlying the purpurogallin‑mediated attenuation of inflammation in BV2 cells closely correlated with the suppression of the translocation of the nuclear factor‑κB (NF‑κB) p65 subunit into the nucleus and the degradation of the inhibitor of NF‑κB (IκB). Moreover, purpurogallin exhibited anti‑inflammatory properties by suppressing the phosphatidylinositol 3‑kinase/Akt and mitogen‑activated protein kinase signaling pathways. These findings suggest that purpurogallin exerts neuroprotective effects through the suppression of pro‑inflammatory pathways in activated microglia.
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Copy and paste a formatted citation
Spandidos Publications style
Park HY, Kim TH, Kim CG, Kim GY, Kim CM, Kim ND, Kim BW, Hwang HJ and Choi YH: Purpurogallin exerts anti‑inflammatory effects in lipopolysaccharide‑stimulated BV2 microglial cells through the inactivation of the NF‑κB and MAPK signaling pathways. Int J Mol Med 32: 1171-1178, 2013.
APA
Park, H.Y., Kim, T.H., Kim, C.G., Kim, G., Kim, C.M., Kim, N.D. ... Choi, Y.H. (2013). Purpurogallin exerts anti‑inflammatory effects in lipopolysaccharide‑stimulated BV2 microglial cells through the inactivation of the NF‑κB and MAPK signaling pathways. International Journal of Molecular Medicine, 32, 1171-1178. https://doi.org/10.3892/ijmm.2013.1478
MLA
Park, H. Y., Kim, T. H., Kim, C. G., Kim, G., Kim, C. M., Kim, N. D., Kim, B. W., Hwang, H. J., Choi, Y. H."Purpurogallin exerts anti‑inflammatory effects in lipopolysaccharide‑stimulated BV2 microglial cells through the inactivation of the NF‑κB and MAPK signaling pathways". International Journal of Molecular Medicine 32.5 (2013): 1171-1178.
Chicago
Park, H. Y., Kim, T. H., Kim, C. G., Kim, G., Kim, C. M., Kim, N. D., Kim, B. W., Hwang, H. J., Choi, Y. H."Purpurogallin exerts anti‑inflammatory effects in lipopolysaccharide‑stimulated BV2 microglial cells through the inactivation of the NF‑κB and MAPK signaling pathways". International Journal of Molecular Medicine 32, no. 5 (2013): 1171-1178. https://doi.org/10.3892/ijmm.2013.1478
Copy and paste a formatted citation
x
Spandidos Publications style
Park HY, Kim TH, Kim CG, Kim GY, Kim CM, Kim ND, Kim BW, Hwang HJ and Choi YH: Purpurogallin exerts anti‑inflammatory effects in lipopolysaccharide‑stimulated BV2 microglial cells through the inactivation of the NF‑κB and MAPK signaling pathways. Int J Mol Med 32: 1171-1178, 2013.
APA
Park, H.Y., Kim, T.H., Kim, C.G., Kim, G., Kim, C.M., Kim, N.D. ... Choi, Y.H. (2013). Purpurogallin exerts anti‑inflammatory effects in lipopolysaccharide‑stimulated BV2 microglial cells through the inactivation of the NF‑κB and MAPK signaling pathways. International Journal of Molecular Medicine, 32, 1171-1178. https://doi.org/10.3892/ijmm.2013.1478
MLA
Park, H. Y., Kim, T. H., Kim, C. G., Kim, G., Kim, C. M., Kim, N. D., Kim, B. W., Hwang, H. J., Choi, Y. H."Purpurogallin exerts anti‑inflammatory effects in lipopolysaccharide‑stimulated BV2 microglial cells through the inactivation of the NF‑κB and MAPK signaling pathways". International Journal of Molecular Medicine 32.5 (2013): 1171-1178.
Chicago
Park, H. Y., Kim, T. H., Kim, C. G., Kim, G., Kim, C. M., Kim, N. D., Kim, B. W., Hwang, H. J., Choi, Y. H."Purpurogallin exerts anti‑inflammatory effects in lipopolysaccharide‑stimulated BV2 microglial cells through the inactivation of the NF‑κB and MAPK signaling pathways". International Journal of Molecular Medicine 32, no. 5 (2013): 1171-1178. https://doi.org/10.3892/ijmm.2013.1478
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