Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
November 2013 Volume 32 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November 2013 Volume 32 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effects of diagnostic ultrasound on HSP70 expression in chorionic villi in rats during early pregnancy and the role of HSP70 in apoptosis in chorionic villi

  • Authors:
    • Haitao Liu
    • Feng Hou
    • Haiying Liang
    • Guocheng Liu
    • Yunfei Li
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Guangdong Provincial Women and Children's Hospital, Guangzhou, Guangdong 511400, P.R. China, Department of Urology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong 511400, P.R. China
  • Pages: 1085-1092
    |
    Published online on: September 12, 2013
       https://doi.org/10.3892/ijmm.2013.1489
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of this study was to investigate the effects of diagnostic ultrasound on chorionic villi during early pregnancy in rats through the examination of heat shock protein 70 (HSP70) expression and apoptosis in chorionic villi. Using TUNEL, RT-PCR, western blot analysis and immunohistochemistry, the extent of apoptosis and the gene/protein expression of HSP70, Bcl-2 and Bax was determined in the samples of the chorionic villus from the rats during early pregnancy. Compared with the unexposed group, there were clear signs that apoptosis had occurred in the villi exposed to ultrasound for 30 min. The expression of HSP70 and Bax in the villi gradually increased with the extended duration of exposure to ultrasound, whereas the expression of Bcl-2 gradually decreased. Bcl-2 expression did not differ significantly between the group exposed for 10 min and the group exposed for 20 min. Taken together, our data demonstrate that HSP70 may protect chorionic villi exposed to ultrasound by inhibiting apoptosis; however, its protective effects are limited over the extended duration of exposure to ultrasound. Thus, the prudent use of ultrasound during the early stages of pregnancy should be advocated.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

ter Haar Gail: Ultrasonic imaging: safety considerations. Interface Focus. 1:686–697. 2011.PubMed/NCBI

2 

Advisory Group on Non-ionising Radiation. Health effects of exposure to ultrasound and infrasound. Health Protection Agency; UK: 2010

3 

Li T, Shi HT, Yang WX and Xiong RP: Effect of diagnostic dose of color doppler ultrasound on apoptosis and Bcl-xl mRNA, Caspase3 mRNA express in rats embryos cells. Zhongguo Yi Xue Wu Li Xue Za Zhi. 3:1242–1245. 2009.(In Chinese).

4 

Luft JC and Dix DJ: Hsp70 expression and function during embryogenesis. Cell Stress Chaperones. 3:162–170. 1999. View Article : Google Scholar : PubMed/NCBI

5 

Hristova I: Role of heat shock proteins (Hsp) in human and mammalian fertilization and pregnancy. Part I Akush Ginekol (Sofiia). 5:45–49. 2012.

6 

Le Masson F and Christians E: HSFs and regulation of Hsp70.1 (Hspa1b) in oocytes and preimplantation embryos: new insights brought by transgenic and knockout mouse models. Cell Stress Chaperones. 3:275–285. 2011.PubMed/NCBI

7 

Sharma L, Kaur J and Shukla G: Role of oxidative stress and apoptosis in the placental pathology of Plasmodium berghei infected mice. PLoS One. 3:e326942012. View Article : Google Scholar : PubMed/NCBI

8 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C (T)) method. Methods. 25:402–408. 2001.

9 

Fowlkes JB; Bioeffects Committee of the American Institute of Ultrasound in Medicine. American Institute of Ultrasound in Medicine consensus report on potential bioeffects of diagnostic ultrasound: executive summary. J Ultrasound Med. 27:503–515. 2008.PubMed/NCBI

10 

Sheiner E, Shoham-Vardi I, Hussey MJ, Pombar X, Strassner HT, Freeman J and Abramowicz JS: First-trimester sonography: is the fetus exposed to high levels of acoustic energy? J Clin Ultrasound. 35:245–249. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Tarantal AF and Hendrickx AG: Evaluation of the bioeffects of prenatal ultrasound exposure in the cynomolgus macaque (Macaca fascicularis). II. Growth and behavior during the first year. Teratology. 39:149–162. 1989.

12 

Ang ES Jr, Gluncic V, Duque A, Schafer ME and Rakic P: Prenatal exposure to ultrasound waves impacts neuronal migration in mice. Proc Natl Acad Sci. 103:12903–12910. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Md Dom S, Abdul Razak HR, Ahmad Zaiki FW, Saat NH, Abd Manan K, Che Isa IN and Hashim UF: Ultrasound exposure during pregnancy affects rabbit foetal parathyroid hormone (PTH) level. Quant Imaging Med Surg. 3:49–53. 2013.PubMed/NCBI

14 

Zhang J, Zhou F, Song Y, Ying W and Zhang Y: Long dwell-time exposure of human chorionic villi to transvaginal ultrasound in the first trimester of pregnancy induces activation of caspase-3 and cytochrome C release. Biol Reprod. 67:580–583. 2002. View Article : Google Scholar

15 

Abramowicz JS, Barnett SB, Duck FA, Edmonds PD, Hynynen KH and Ziskin MC: Fetal thermal effects of diagnostic ultrasound. J Ultrasound Med. 27:541–563. 2008.PubMed/NCBI

16 

Ziskin MC and Barnett SB: Ultrasound and the developing nervous system. Ultrasound Med Biol. 27:875–876. 2001. View Article : Google Scholar

17 

Driver AM and Khatib H: Physiology and Endocrinology Symposium: heat shock proteins: potentially powerful markers for preimplantation embryonic development and fertility in livestock species. J Anim Sci. 91:1154–1161. 2013. View Article : Google Scholar

18 

Gupta SC, Sharma A, Mishra M, Mishra RK and Chowdhuri DK: Heat shock proteins in toxicology: how close and how far? Life Sci. 86:377–384. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Zhang B, Peñagaricano F, Driver A, Chen H and Khatib H: Differential expression of heat shock protein genes and their splice variants in bovine preimplantation embryos. J Dairy Sci. 94:4174–4182. 2011. View Article : Google Scholar : PubMed/NCBI

20 

King AM and MacRae TH: The small heat shock protein p26 aids development of encysting Artemia embryos, prevents spontaneous diapause termination and protects against stress. PLoS One. 7:e437232012. View Article : Google Scholar : PubMed/NCBI

21 

Bradley E, Bieberich E, Mivechi NF, Tangpisuthipongsa D and Wang G: Regulation of embryonic stem cell pluripotency by heat shock protein 90. Stem Cells. 8:1624–1633. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Mayer MP and Bukau B: Hsp70 chaperones, cellular functions and molecular mechanism. Cell Mol Life Sci. 62:670–684. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Welch WJ: How cells respond to stress. Sci Am. 268:56–64. 1993. View Article : Google Scholar : PubMed/NCBI

24 

Lindquist S and Craig EA: The heat shock proteins. Annu Rev Genet. 22:631–677. 1988. View Article : Google Scholar

25 

Rokutan K, Hirakawa T, Teshima S, et al: Implications of heat shock/stress proteins for medicine and disease. J Med Invest. 44:137–147. 1998.PubMed/NCBI

26 

Neuer A, Mele C, Liu HC, et al: Monoclonal antibodies to mammalian heat shock proteins impair mouse embryo development in vitro. Hum Reprod. 4:987–990. 1998. View Article : Google Scholar : PubMed/NCBI

27 

Ola MS, Nawaz M and Ahsan H: Role of Bcl-2 family proteins and caspases in the regulation of apoptosis. Mol Cell Biochem. 351:41–58. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Kelly S, Zhang ZJ, Zhao H, Xu L, et al: Gene transfer of HSP72 protects cornu ammonis 1 region of the hippocampus neurons from global ischemia: influence of Bcl-2. Ann Neurol. 52:160–167. 2002. View Article : Google Scholar : PubMed/NCBI

29 

Nagashima M, Fujikawa C, Mawatari K, Mori Y and Kato S: HSP70, the earliest-induced gene in the zebrafish retina during optic nerve regeneration: its role in cell survival. Neurochem Int. 58:888–895. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Sharp FR, Massa SM and Swanson RA: Heat-shock protein protection. Trends Neurosci. 22:97–99. 1999. View Article : Google Scholar : PubMed/NCBI

31 

Tanaka K and Mizushima T: Protective role of HSF1 and HSP70 against gastrointestinal diseases. Int J Hyperthermia. 25:668–676. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Kim MK, Fibrianto YH, Oh HJ, et al: Effect of beta-mercaptoethanol or epidermal growth factor supplementation on in vitro maturation of canine oocytes collected from dogs with different stages of the estrus cycle. J Vet Sci. 5:253–258. 2004.PubMed/NCBI

33 

Glabowski W, Wiszniewska B and Kurzawa R: Protective potential of SCF for mice preimplantation embryos cultured in vitro in suboptimal conditions. J Assist Reprod Genet. 25:395–402. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Bonilla AQ, Oliveira LJ, Ozawa M, Newsom EM, Lucy MC and Hansen PJ: Developmental changes in thermoprotective actions of insulin-like growth factor-1 on the preimplantation bovine embryo. Mol Cell Endocrinol. 332:170–179. 2011. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Liu H, Hou F, Liang H, Liu G and Li Y: Effects of diagnostic ultrasound on HSP70 expression in chorionic villi in rats during early pregnancy and the role of HSP70 in apoptosis in chorionic villi. Int J Mol Med 32: 1085-1092, 2013.
APA
Liu, H., Hou, F., Liang, H., Liu, G., & Li, Y. (2013). Effects of diagnostic ultrasound on HSP70 expression in chorionic villi in rats during early pregnancy and the role of HSP70 in apoptosis in chorionic villi. International Journal of Molecular Medicine, 32, 1085-1092. https://doi.org/10.3892/ijmm.2013.1489
MLA
Liu, H., Hou, F., Liang, H., Liu, G., Li, Y."Effects of diagnostic ultrasound on HSP70 expression in chorionic villi in rats during early pregnancy and the role of HSP70 in apoptosis in chorionic villi". International Journal of Molecular Medicine 32.5 (2013): 1085-1092.
Chicago
Liu, H., Hou, F., Liang, H., Liu, G., Li, Y."Effects of diagnostic ultrasound on HSP70 expression in chorionic villi in rats during early pregnancy and the role of HSP70 in apoptosis in chorionic villi". International Journal of Molecular Medicine 32, no. 5 (2013): 1085-1092. https://doi.org/10.3892/ijmm.2013.1489
Copy and paste a formatted citation
x
Spandidos Publications style
Liu H, Hou F, Liang H, Liu G and Li Y: Effects of diagnostic ultrasound on HSP70 expression in chorionic villi in rats during early pregnancy and the role of HSP70 in apoptosis in chorionic villi. Int J Mol Med 32: 1085-1092, 2013.
APA
Liu, H., Hou, F., Liang, H., Liu, G., & Li, Y. (2013). Effects of diagnostic ultrasound on HSP70 expression in chorionic villi in rats during early pregnancy and the role of HSP70 in apoptosis in chorionic villi. International Journal of Molecular Medicine, 32, 1085-1092. https://doi.org/10.3892/ijmm.2013.1489
MLA
Liu, H., Hou, F., Liang, H., Liu, G., Li, Y."Effects of diagnostic ultrasound on HSP70 expression in chorionic villi in rats during early pregnancy and the role of HSP70 in apoptosis in chorionic villi". International Journal of Molecular Medicine 32.5 (2013): 1085-1092.
Chicago
Liu, H., Hou, F., Liang, H., Liu, G., Li, Y."Effects of diagnostic ultrasound on HSP70 expression in chorionic villi in rats during early pregnancy and the role of HSP70 in apoptosis in chorionic villi". International Journal of Molecular Medicine 32, no. 5 (2013): 1085-1092. https://doi.org/10.3892/ijmm.2013.1489
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team