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Article

The in vitro and in vivo treatment effects of overexpressed lentiviral vector-mediated human BMP2 gene in the femoral bone marrow stromal cells of osteoporotic rats

  • Authors:
    • Bing Zhao
    • Qiang Wang
    • Tianzun Tao
    • Juan Li
    • Qingyuan Lin
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, the Second Hospital Affiliated to Harbin Medical University, Harbin 150081, P.R. China, Department of Clinical Laboratory Medicine, the Second Hospital Affiliated to Harbin Medical University, Harbin 150081, P.R. China, Department of Immunology, Harbin Medical University, Harbin 150081, P.R. China
  • Pages: 1355-1365
    |
    Published online on: September 25, 2013
       https://doi.org/10.3892/ijmm.2013.1507
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Abstract

This study aimed to compare the treatment effects of lentiviral vector-mediated hBMP2 which was overexpressed in the femoral bone marrow stromal cells of osteoporotic rats through genetic infection in vitro and in vivo. Comparison of the two transgenic effects may be crucial to determining the lentivirus infection method to be used. Following a comparison of the rat bone marrow stromal cells (rBMSCs) in osteoporotic (MSCs OVX) and normal (MSCs CON) groups, the lentiviral vector-mediated human bone morphogenetic protein 2 (hBMP2), which overexpressed the BMSCs of osteoporotic rats in vitro (rBMSCs in OE group), was constructed. The osteogenic ability in the overexpressed (OE) group was then compared to that of the MSCs CON. The rBMSCs in the OE group (transplants of genetic infection in vitro) and the lentivirus-containing solution (injected material of genetic infection in vivo) were injected into the femurs. The treatment effect of each group was compared via bone mineral density (BMD) and bone histomorphometry. The hBMP2-modified osteoporosis rBMSCs formed by genetic infection in vitro (n=7) had an ameliorated treatment effect on the femur as compared to that of the in vivo (n=7) (BMD: 0.315 vs. 0.19 g/cm2, P<0.01; bone histomorphometry: For bone trabeculars (Tb.Ar/T.Ar): 0.301 vs. 0.114, P<0.01; for trabecular thickness (Tb.Th): 43.54 vs. 21.39 µm, P<0.01; for trabecular separation (Tb.Sp): 115.7 vs. 304.87 µm, P<0.01). The results showed that the treatment effects of osteoporotic rBMSCs on local osteoporosis performed by genetic infection were improved in vitro as compared to those in vivo.
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View References

1 

Kim S, Kang Y, Krueger CA, et al: Sequential delivery of BMP-2 and IGF-1 using a chitosan gel with gelatin microspheres enhances early osteoblastic differentiation. Acta Biomater. 8:1768–1777. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Song I, Kim BS, Kim CS and Im GI: Effects of BMP-2 and vitamin D3 on the osteogenic differentiation of adipose stem cells. Biochem Biophys Res Commun. 408:126–131. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Edgar CM, Chakravarthy V, Barnes G, Kakar S, Gerstenfeld LC and Einhorn TA: Autogenous regulation of a network of bone morphogenetic proteins (BMPs) mediates the osteogenic differentiation in murine marrow stromal cells. Bone. 40:1389–1398. 2007. View Article : Google Scholar

4 

Ben-David D, Srouji S, Shapira-Schweitzer K, et al: Low dose BMP-2 treatment for bone repair using a PEGylated fibrinogen hydrogel matrix. Biomaterials. 34:2902–2910. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Tsuji K, Bandyopadhyay A, Harfe BD, et al: BMP2 activity, although dispensable for bone formation, is required for the initiation of fracture healing. Nat Genet. 38:1424–1429. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Matsumoto A, Yamaji K, Kawanami M and Kato H: Effect of aging on bone formation induced by recombinant human bone morphogenetic protein-2 combined with fibrous collagen membranes at subperiosteal sites. J Periodontal Res. 36:175–182. 2001. View Article : Google Scholar

7 

Bai Y, Li P, Yin G, et al: BMP-2, VEGF and bFGF synergistically promote the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells. Biotechnol Lett. 35:301–308. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Lieberman JR, Daluiski A, Stevenson S, et al: The effect of regional gene therapy with bone morphogenetic protein-2-producing bone-marrow cells on the repair of segmental femoral defects in rats. J Bone Joint Surg Am. 81:905–917. 1999.PubMed/NCBI

9 

Tsuda H, Wada T, Ito Y, et al: Efficient BMP2 gene transfer and bone formation of mesenchymal stem cells by a fiber-mutant adenoviral vector. Mol Ther. 7:354–365. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Jiang J, Fan CY and Zeng BF: Osteogenic differentiation effects on rat bone marrow-derived mesenchymal stromal cells by lentivirus-mediated co-transfection of human BMP2 gene and VEGF165 gene. Biotechnol Lett. 30:197–203. 2008. View Article : Google Scholar

11 

Zhu C, Chang Q, Zou D, et al: LvBMP-2 gene-modified BMSCs combined with calcium phosphate cement scaffolds for the repair of calvarial defects in rats. J Mater Sci Mater Med. 22:1965–1973. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Hsu WK, Sugiyama O, Park SH, et al: Lentiviral-mediated BMP-2 gene transfer enhances healing of segmental femoral defects in rats. Bone. 40:931–938. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Oluigbo CO and Solanki GA: Use of recombinant human bone morphogenetic protein-2 to enhance posterior cervical spine fusion at 2 years of age: technical note. Pediatr Neurosurg. 44:393–396. 2008.PubMed/NCBI

14 

Zara JN, Siu RK, Zhang X, et al: High doses of bone morphogenetic protein 2 induce structurally abnormal bone and inflammation in vivo. Tissue Eng Part A. 17:1389–1399. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Tsuda H, Wada T, Yamashita T and Hamada H: Enhanced osteoinduction by mesenchymal stem cells transfected with a fiber- mutant adenoviral BMP2 gene. J Gene Med. 7:1322–1334. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Yukawa Y, Lou J, Fukui N and Lenke LG: Optimal treatment timing to attenuate neuronal apoptosis via Bcl-2 gene transfer in vitro and in vivo. J Neurotrauma. 19:1091–1103. 2002. View Article : Google Scholar : PubMed/NCBI

17 

Sapet C, Pellegrino C, Laurent N, Sicard F and Zelphati O: Magnetic nanoparticles enhance adenovirus transduction in vitro and in vivo. Pharm Res. 29:1203–1218. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Zhang L, Liu HJ, Li TJ, et al: Lentiviral vector-mediated siRNA knockdown of SR-PSOX inhibits foam cell formation in vitro. Acta Pharmacol Sin. 29:847–852. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Zhou S, Mizuno S and Glowacki J: Wnt pathway regulation by demineralized bone is approximated by both BMP-2 and TGF-β1 signaling. J Orthop Res. 31:554–560. 2013.PubMed/NCBI

20 

Jiang XQ, Chen JG, Gittens S, Chen CJ, Zhang XL and Zhang ZY: The ectopic study of tissue-engineered bone with hBMP-4 gene modified bone marrow stromal cells in rabbits. Chin Med J (Engl). 118:281–288. 2005.PubMed/NCBI

21 

Krebsbach PH, Zhang K, Malik AK and Kurachi K: Bone marrow stromal cells as a genetic platform for systemic delivery of therapeutic proteins in vivo: human factor IX model. J Gene Med. 5:11–17. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Hao L, Wang J, Zou Z, et al: Transplantation of BMSCs expressing hPDGF-A/hBD2 promotes wound healing in rats with combined radiation-wound injury. Gene Ther. 16:34–42. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Huang Q, Liu XZ, Kang CS, Wang GX, Zhong Y and Pu PY: The anti-glioma effect of suicide gene therapy using BMSC expressing HSV/TK combined with overexpression of Cx43 in glioma cells. Cancer Gene Ther. 17:192–202. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Maniatopoulos C, Sodek J and Melcher AH: Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats. Cell Tissue Res. 254:317–330. 1988. View Article : Google Scholar : PubMed/NCBI

25 

Zou D, Han W, You S, et al: In vitro study of enhanced osteogenesis induced by HIF-1α-transduced bone marrow stem cells. Cell Prolif. 44:234–243. 2011.

26 

Nishida S, Endo N, Yamagiwa H, Tanizawa T and Takahashi HE: Number of osteoprogenitor cells in human bone marrow markedly decreases after skeletal maturation. J Bone Miner Metab. 17:171–177. 1999. View Article : Google Scholar : PubMed/NCBI

27 

Liu HC, ELL, Wang DS, et al: Reconstruction of alveolar bone defects using bone morphogenetic protein 2 mediated rabbit dental pulp stem cells seeded on nano-hydroxyapatite/collagen/poly(L-lactide). Tissue Eng Part A. 17:2417–2433. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Tamminen IS, Isaksson H, Aula AS, Honkanen E, Jurvelin JS and Kröger H: Reproducibility and agreement of micro-CT and histomorphometry in human trabecular bone with different metabolic status. J Bone Miner Metab. 29:442–448. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Hapidin H, Othman F, Soelaiman IN, Shuid AN and Mohamed N: Effects of nicotine administration and nicotine cessation on bone histomorphometry and bone biomarkers in Sprague-Dawley male rats. Calcif Tissue Int. 88:41–47. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Owen M and Friedenstein AJ: Stromal stem cells: marrow-derived osteogenic precursors. Ciba Found Symp. 136:42–60. 1988.PubMed/NCBI

31 

Derubeis AR and Cancedda R: Bone marrow stromal cells (BMSCs) in bone engineering: limitations and recent advances. Ann Biomed Eng. 32:160–165. 2004. View Article : Google Scholar : PubMed/NCBI

32 

Zou L, Zou X, Chen L, et al: Multilineage differentiation of porcine bone marrow stromal cells associated with specific gene expression pattern. J Orthop Res. 26:56–64. 2008. View Article : Google Scholar : PubMed/NCBI

33 

Stucki U, Schmid J, Hämmerle CF and Lang NP: Temporal and local appearance of alkaline phosphatase activity in early stages of guided bone regeneration. A descriptive histochemical study in humans. Clin Oral Implants Res. 12:121–127. 2001. View Article : Google Scholar : PubMed/NCBI

34 

Luu HH, Song WX, Luo X, et al: Distinct roles of bone morphogenetic proteins in osteogenic differentiation of mesenchymal stem cells. J Orthop Res. 25:665–677. 2007. View Article : Google Scholar : PubMed/NCBI

35 

Ying X, Cheng S, Wang W, et al: Effect of boron on osteogenic differentiation of human bone marrow stromal cells. Biol Trace Elem Res. 144:306–315. 2011. View Article : Google Scholar : PubMed/NCBI

36 

Li DJ, Ge DX, Wu WC, Wu J and Li L: Osteogenic potential of bone marrow mesenchymal stem cells from ovariectomied osteoporotic rat. Sichuan Da Xue Xue Bao Yi Xue Ban. 36:318–321. 2005.(In Chinese).

37 

Vaidya R, Weir R, Sethi A, Meisterling S, Hakeos W and Wybo CD: Interbody fusion with allograft and rhBMP-2 leads to consistent fusion but early subsidence. J Bone Joint Surg Br. 89:342–345. 2007. View Article : Google Scholar : PubMed/NCBI

38 

Vaidya R, Carp J, Sethi A, Bartol S, Craig J and Les CM: Complications of anterior cervical discectomy and fusion using recombinant human bone morphogenetic protein-2. Eur Spine J. 16:1257–1265. 2007. View Article : Google Scholar : PubMed/NCBI

39 

Irie K, Alpaslan C, Takahashi K, et al: Osteoclast differentiation in ectopic bone formation induced by recombinant human bone morphogenetic protein 2 (rhBMP-2). J Bone Miner Metab. 21:363–369. 2003. View Article : Google Scholar : PubMed/NCBI

40 

Wan Y, Chong LW and Evans RM: PPAR-gamma regulates osteoclastogenesis in mice. Nat Med. 13:1496–1503. 2007. View Article : Google Scholar : PubMed/NCBI

41 

Glass DA II and Karsenty G: Canonical Wnt signaling in osteoblasts is required for osteoclast differentiation. Ann N Y Acad Sci. 1068:117–130. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhao B, Wang Q, Tao T, Li J and Lin Q: The in vitro and in vivo treatment effects of overexpressed lentiviral vector-mediated human BMP2 gene in the femoral bone marrow stromal cells of osteoporotic rats. Int J Mol Med 32: 1355-1365, 2013.
APA
Zhao, B., Wang, Q., Tao, T., Li, J., & Lin, Q. (2013). The in vitro and in vivo treatment effects of overexpressed lentiviral vector-mediated human BMP2 gene in the femoral bone marrow stromal cells of osteoporotic rats. International Journal of Molecular Medicine, 32, 1355-1365. https://doi.org/10.3892/ijmm.2013.1507
MLA
Zhao, B., Wang, Q., Tao, T., Li, J., Lin, Q."The in vitro and in vivo treatment effects of overexpressed lentiviral vector-mediated human BMP2 gene in the femoral bone marrow stromal cells of osteoporotic rats". International Journal of Molecular Medicine 32.6 (2013): 1355-1365.
Chicago
Zhao, B., Wang, Q., Tao, T., Li, J., Lin, Q."The in vitro and in vivo treatment effects of overexpressed lentiviral vector-mediated human BMP2 gene in the femoral bone marrow stromal cells of osteoporotic rats". International Journal of Molecular Medicine 32, no. 6 (2013): 1355-1365. https://doi.org/10.3892/ijmm.2013.1507
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao B, Wang Q, Tao T, Li J and Lin Q: The in vitro and in vivo treatment effects of overexpressed lentiviral vector-mediated human BMP2 gene in the femoral bone marrow stromal cells of osteoporotic rats. Int J Mol Med 32: 1355-1365, 2013.
APA
Zhao, B., Wang, Q., Tao, T., Li, J., & Lin, Q. (2013). The in vitro and in vivo treatment effects of overexpressed lentiviral vector-mediated human BMP2 gene in the femoral bone marrow stromal cells of osteoporotic rats. International Journal of Molecular Medicine, 32, 1355-1365. https://doi.org/10.3892/ijmm.2013.1507
MLA
Zhao, B., Wang, Q., Tao, T., Li, J., Lin, Q."The in vitro and in vivo treatment effects of overexpressed lentiviral vector-mediated human BMP2 gene in the femoral bone marrow stromal cells of osteoporotic rats". International Journal of Molecular Medicine 32.6 (2013): 1355-1365.
Chicago
Zhao, B., Wang, Q., Tao, T., Li, J., Lin, Q."The in vitro and in vivo treatment effects of overexpressed lentiviral vector-mediated human BMP2 gene in the femoral bone marrow stromal cells of osteoporotic rats". International Journal of Molecular Medicine 32, no. 6 (2013): 1355-1365. https://doi.org/10.3892/ijmm.2013.1507
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