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Article

Probiotic-fermented purple sweet potato yogurt activates compensatory IGF‑IR/PI3K/Akt survival pathways and attenuates cardiac apoptosis in the hearts of spontaneously hypertensive rats

  • Authors:
    • Pei-Pei Lin
    • You-Miin Hsieh
    • Wei-Wen Kuo
    • Yueh-Min Lin
    • Yu-Lan Yeh
    • Chien-Chung Lin
    • Fuu-Jen Tsai
    • Chang-Hai Tsai
    • Chih-Yang Huang
    • Cheng-Chih Tsai
  • View Affiliations / Copyright

    Affiliations: Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C., Department of Food and Nutrition, Providence University, Taichung, Taiwan, R.O.C., Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C., Department of Pathology, Changhua Christian Hospital, Changhua, Taiwan, R.O.C., Department of Orthopaedics, Armed Forces General Hospital, Taichung, Taiwan, R.O.C., Graduate Institute of Chinese Medical Science, Medical Research and Medical Genetics, China Medical University, Taichung, Taiwan, R.O.C., Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, R.O.C., Department of Food Science and Technology, HungKuang University, Taichung, Taiwan, R.O.C.
  • Pages: 1319-1328
    |
    Published online on: October 11, 2013
       https://doi.org/10.3892/ijmm.2013.1524
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Abstract

Apoptosis is recognized as a predictor of adverse outcomes in subjects with cardiac diseases. The aim of this study was to explore the effects of probiotic-fermented purple sweet potato yogurt (PSPY) with high γ-aminobutyric acid (GABA) content on cardiac apoptosis in spontaneously hypertensive rat (SHR) hearts. The rats were orally adminsitered with 2 different concentrations of PSPY (10 and 100%) or captopril, 15.6 mg/kg, body weight (BW)/day. The control group was administered distilled water. DAPI and TUNEL staining were used to detect the numbers of apoptotic cells. A decrease in the number of TUNEL-positive cardiac myocytes was observed in the SHR-PSPY (10 and 100%) groups. In addition, the levels of key components of the Fas receptor- and mitochondrial-dependent apoptotic pathways were determined by western blot analysis. The results revealed that the levels of the key components of the Fas receptor- and mitochondrial-dependent apoptotic pathway were significantly decreased in the SHR-captopril, and 10 and 100% PSPY groups. Additionally, the levels of phosphorylated insulin-like growth factor‑I receptor (p-IGF‑IR) were increased in SHR hearts from the SHR-control group; however, no recovery in the levels of downstream signaling components was observed. In addition, the levels of components of the compensatory IGF-IR-dependent survival pathway (p-PI3K and p-Akt) were all highly enhanced in the left ventricles in the hearts form the SHR-10 and 100% PSPY groups. Therefore, the oral administration of PSPY may attenuate cardiomyocyte apoptosis in SHR hearts by activating IGF‑IR-dependent survival signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Lin P, Hsieh Y, Kuo W, Lin Y, Yeh Y, Lin C, Tsai F, Tsai C, Huang C, Tsai C, Tsai C, et al: Probiotic-fermented purple sweet potato yogurt activates compensatory IGF‑IR/PI3K/Akt survival pathways and attenuates cardiac apoptosis in the hearts of spontaneously hypertensive rats. Int J Mol Med 32: 1319-1328, 2013.
APA
Lin, P., Hsieh, Y., Kuo, W., Lin, Y., Yeh, Y., Lin, C. ... Tsai, C. (2013). Probiotic-fermented purple sweet potato yogurt activates compensatory IGF‑IR/PI3K/Akt survival pathways and attenuates cardiac apoptosis in the hearts of spontaneously hypertensive rats. International Journal of Molecular Medicine, 32, 1319-1328. https://doi.org/10.3892/ijmm.2013.1524
MLA
Lin, P., Hsieh, Y., Kuo, W., Lin, Y., Yeh, Y., Lin, C., Tsai, F., Tsai, C., Huang, C., Tsai, C."Probiotic-fermented purple sweet potato yogurt activates compensatory IGF‑IR/PI3K/Akt survival pathways and attenuates cardiac apoptosis in the hearts of spontaneously hypertensive rats". International Journal of Molecular Medicine 32.6 (2013): 1319-1328.
Chicago
Lin, P., Hsieh, Y., Kuo, W., Lin, Y., Yeh, Y., Lin, C., Tsai, F., Tsai, C., Huang, C., Tsai, C."Probiotic-fermented purple sweet potato yogurt activates compensatory IGF‑IR/PI3K/Akt survival pathways and attenuates cardiac apoptosis in the hearts of spontaneously hypertensive rats". International Journal of Molecular Medicine 32, no. 6 (2013): 1319-1328. https://doi.org/10.3892/ijmm.2013.1524
Copy and paste a formatted citation
x
Spandidos Publications style
Lin P, Hsieh Y, Kuo W, Lin Y, Yeh Y, Lin C, Tsai F, Tsai C, Huang C, Tsai C, Tsai C, et al: Probiotic-fermented purple sweet potato yogurt activates compensatory IGF‑IR/PI3K/Akt survival pathways and attenuates cardiac apoptosis in the hearts of spontaneously hypertensive rats. Int J Mol Med 32: 1319-1328, 2013.
APA
Lin, P., Hsieh, Y., Kuo, W., Lin, Y., Yeh, Y., Lin, C. ... Tsai, C. (2013). Probiotic-fermented purple sweet potato yogurt activates compensatory IGF‑IR/PI3K/Akt survival pathways and attenuates cardiac apoptosis in the hearts of spontaneously hypertensive rats. International Journal of Molecular Medicine, 32, 1319-1328. https://doi.org/10.3892/ijmm.2013.1524
MLA
Lin, P., Hsieh, Y., Kuo, W., Lin, Y., Yeh, Y., Lin, C., Tsai, F., Tsai, C., Huang, C., Tsai, C."Probiotic-fermented purple sweet potato yogurt activates compensatory IGF‑IR/PI3K/Akt survival pathways and attenuates cardiac apoptosis in the hearts of spontaneously hypertensive rats". International Journal of Molecular Medicine 32.6 (2013): 1319-1328.
Chicago
Lin, P., Hsieh, Y., Kuo, W., Lin, Y., Yeh, Y., Lin, C., Tsai, F., Tsai, C., Huang, C., Tsai, C."Probiotic-fermented purple sweet potato yogurt activates compensatory IGF‑IR/PI3K/Akt survival pathways and attenuates cardiac apoptosis in the hearts of spontaneously hypertensive rats". International Journal of Molecular Medicine 32, no. 6 (2013): 1319-1328. https://doi.org/10.3892/ijmm.2013.1524
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