In vitro assessment of the dual-targeting behavior of a peptide-based magnetic resonance imaging contrast agent

  • Authors:
    • Yuping Yang
    • Kaichao Yu
    • Hailu Zhang
    • Jianwu Dai
    • Zongwu Deng
  • View Affiliations

  • Published online on: November 7, 2013     https://doi.org/10.3892/ijmm.2013.1551
  • Pages: 215-220
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Abstract

In this study, a peptide-based dual-targeting magnetic resonance imaging (MRI) contrast agent (S8) was designed and synthesized. Arg-Gly-Asp (RGD) and Asn-Gly-Arg (NGR) were combined in the targeting vector so as to allow binding, on the surface of tumor cells, to integrin αvβ3 and aminopeptidase N (CD13), respectively. The longitudinal relaxivity (r1) value of S8 was 8.297 mM-1sec-1 at a magnetic field of 11.7 T, which is approximately double the r1 value (4.25 mM-1sec-1) of Magnevist, a commercially available contrast agent. MDA-MB-231 human breast cancer cells (which overexpress αvβ3) and human prostate cancer cells PC-3 (which overexpress CD13) were used to investigate the tumor‑targeting behavior of S8. The results from the present study indicate that the designed contrast agent, S8, targets both MDA-MB‑231 and PC-3 cells.
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2014-January
Volume 33 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yang Y, Yu K, Zhang H, Dai J and Deng Z: In vitro assessment of the dual-targeting behavior of a peptide-based magnetic resonance imaging contrast agent. Int J Mol Med 33: 215-220, 2014
APA
Yang, Y., Yu, K., Zhang, H., Dai, J., & Deng, Z. (2014). In vitro assessment of the dual-targeting behavior of a peptide-based magnetic resonance imaging contrast agent. International Journal of Molecular Medicine, 33, 215-220. https://doi.org/10.3892/ijmm.2013.1551
MLA
Yang, Y., Yu, K., Zhang, H., Dai, J., Deng, Z."In vitro assessment of the dual-targeting behavior of a peptide-based magnetic resonance imaging contrast agent". International Journal of Molecular Medicine 33.1 (2014): 215-220.
Chicago
Yang, Y., Yu, K., Zhang, H., Dai, J., Deng, Z."In vitro assessment of the dual-targeting behavior of a peptide-based magnetic resonance imaging contrast agent". International Journal of Molecular Medicine 33, no. 1 (2014): 215-220. https://doi.org/10.3892/ijmm.2013.1551