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International Journal of Molecular Medicine
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Article

In vitro assessment of the dual-targeting behavior of a peptide-based magnetic resonance imaging contrast agent

  • Authors:
    • Yuping Yang
    • Kaichao Yu
    • Hailu Zhang
    • Jianwu Dai
    • Zongwu Deng
  • View Affiliations / Copyright

    Affiliations: School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, Hubei 430007, P.R. China, Division of Nanobionics, Suzhou Institute of Nanotech and Nanobionics, Chinese Academy of Sciences, Suzhou, Jiangsu 215123, P.R. China, Division of Nanobiomedicine, Suzhou Institute of Nanotech and Nanobionics, Chinese Academy of Sciences, Suzhou, Jiangsu 215123, P.R. China
  • Pages: 215-220
    |
    Published online on: November 7, 2013
       https://doi.org/10.3892/ijmm.2013.1551
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Abstract

In this study, a peptide-based dual-targeting magnetic resonance imaging (MRI) contrast agent (S8) was designed and synthesized. Arg-Gly-Asp (RGD) and Asn-Gly-Arg (NGR) were combined in the targeting vector so as to allow binding, on the surface of tumor cells, to integrin αvβ3 and aminopeptidase N (CD13), respectively. The longitudinal relaxivity (r1) value of S8 was 8.297 mM-1sec-1 at a magnetic field of 11.7 T, which is approximately double the r1 value (4.25 mM-1sec-1) of Magnevist, a commercially available contrast agent. MDA-MB-231 human breast cancer cells (which overexpress αvβ3) and human prostate cancer cells PC-3 (which overexpress CD13) were used to investigate the tumor‑targeting behavior of S8. The results from the present study indicate that the designed contrast agent, S8, targets both MDA-MB‑231 and PC-3 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Yu K, Zhang H, Dai J and Deng Z: In vitro assessment of the dual-targeting behavior of a peptide-based magnetic resonance imaging contrast agent. Int J Mol Med 33: 215-220, 2014.
APA
Yang, Y., Yu, K., Zhang, H., Dai, J., & Deng, Z. (2014). In vitro assessment of the dual-targeting behavior of a peptide-based magnetic resonance imaging contrast agent. International Journal of Molecular Medicine, 33, 215-220. https://doi.org/10.3892/ijmm.2013.1551
MLA
Yang, Y., Yu, K., Zhang, H., Dai, J., Deng, Z."In vitro assessment of the dual-targeting behavior of a peptide-based magnetic resonance imaging contrast agent". International Journal of Molecular Medicine 33.1 (2014): 215-220.
Chicago
Yang, Y., Yu, K., Zhang, H., Dai, J., Deng, Z."In vitro assessment of the dual-targeting behavior of a peptide-based magnetic resonance imaging contrast agent". International Journal of Molecular Medicine 33, no. 1 (2014): 215-220. https://doi.org/10.3892/ijmm.2013.1551
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Yu K, Zhang H, Dai J and Deng Z: In vitro assessment of the dual-targeting behavior of a peptide-based magnetic resonance imaging contrast agent. Int J Mol Med 33: 215-220, 2014.
APA
Yang, Y., Yu, K., Zhang, H., Dai, J., & Deng, Z. (2014). In vitro assessment of the dual-targeting behavior of a peptide-based magnetic resonance imaging contrast agent. International Journal of Molecular Medicine, 33, 215-220. https://doi.org/10.3892/ijmm.2013.1551
MLA
Yang, Y., Yu, K., Zhang, H., Dai, J., Deng, Z."In vitro assessment of the dual-targeting behavior of a peptide-based magnetic resonance imaging contrast agent". International Journal of Molecular Medicine 33.1 (2014): 215-220.
Chicago
Yang, Y., Yu, K., Zhang, H., Dai, J., Deng, Z."In vitro assessment of the dual-targeting behavior of a peptide-based magnetic resonance imaging contrast agent". International Journal of Molecular Medicine 33, no. 1 (2014): 215-220. https://doi.org/10.3892/ijmm.2013.1551
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