Detection of mitochondrial DNA mutations by high-throughput sequencing in the blood of breast cancer patients

Li,Lin   Hai; Kang,Tao; Chen,Lidan; Zhang,Weiyun; Liao,Yang; Chen,Jianyun; Shi,Yuling

doi:10.3892/ijmm.2013.1559

2014-January Volume 33 Issue 1

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2014-January Volume 33 Issue 1

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Article

Detection of mitochondrial DNA mutations by high-throughput sequencing in the blood of breast cancer patients

Authors:
- Lin Hai Li
- Tao Kang
- Lidan Chen
- Weiyun Zhang
- Yang Liao
- Jianyun Chen
- Yuling Shi
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Affiliations: Graduate School of Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, International Center for Metabolic Diseases, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Laboratory Medicine, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010, P.R. China
Pages: 77-82
|
Published online on: November 19, 2013

https://doi.org/10.3892/ijmm.2013.1559
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Abstract

Mitochondrial DNA mutations have been identified in serveral types of cancer. In breast cancer, germline and somatic mitochondrial DNA (mtDNA) mutations have been identified. A number of mtDNA mutations in breast cancer have been identified in protein-coding regions (in protein-coding genes, such as ND2, COX3, ND4, ND5 and CytB). Mutations in these structure proteins cause impaired electron transport function and lead to electron leakage and increased reactive oxygen species (ROS) production, which in turn increases oxidative stress and oxidative damage to the mitochondria, as well as to cells. These data establish an association between mtDNA mutations and breast cancer; however, there is no reliable prediction of breast cancer predisposition or progression based on mtDNA mutation patterns thus far. In this study, we used high-throughput sequencing to detect mtDNA mutations in the blood of breast cancer patients. Some of these mutations may be used as potential markers for breast cancer diagnosis.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Detection of mitochondrial DNA mutations by high-throughput sequencing in the blood of breast cancer patients

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Abstract

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