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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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2014-March Volume 33 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Overexpression of GNAO1 correlates with poor prognosis in patients with gastric cancer and plays a role in gastric cancer cell proliferation and apoptosis

  • Authors:
    • Zhen Liu
    • Jun Zhang
    • Lijun Wu
    • Jie Liu
    • Mingqing Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Digestive Diseases, Southeast Hospital, Xiamen University, Zhangzhou, Fujian, P.R. China, Department of Digestive Diseases, Huashan Hospital, Fudan University, Shanghai, P.R. China
  • Pages: 589-596
    |
    Published online on: December 20, 2013
       https://doi.org/10.3892/ijmm.2013.1598
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Abstract

Guanine nucleotide-binding protein (G protein), alpha activating activity polypeptide O (GNAO1) encodes an alpha subunit of the heterotrimeric guanine nucleotide-binding proteins (Gαo), which plays a significant role in the development of various types of cancer, including breast cancer and hepatocellular carcinoma. However, its role in gastric cancer (GC) has not yet been elucidated. In the current study, the expression of Gαo was analyzed by immunohistochemistry in paraffin-embedded tissue sections from 70 patients with GC and its cellular role was examined by small interfering RNA (siRNA)-mediated downregulation. The overexpression of GNAO1 (scores of 2 or 4) was observed in 44 of the 70 patients with GC (62.9%) and the expression of Gαo significantly correlated with the overall survival of the patients with GC after surgery. The median survival rate of patients with a negative or positive expression of Gαo was 61 or 27 months, respectively (P=0.033). The silencing of GNAO1 in GC cells by RNA interference markedly inhibited the proliferation of GC cells and promoted apoptosis by increasing the accumulation of the pro-apoptotic proteins, Puma and Bim, possibly mediated by extracellular signal-regulated kinase 1 and 2 (ERK1/2). This further confirmed the clinical significance of GNAO1, which was overexpressed in GC tissues. To the best of our knowledge, the present study is the first to demonstrate that GNAO1 is overexpressed in GC and that its overexpression correlates with poor prognosis, as it promotes GC cell viability.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Z, Zhang J, Wu L, Liu J and Zhang M: Overexpression of GNAO1 correlates with poor prognosis in patients with gastric cancer and plays a role in gastric cancer cell proliferation and apoptosis. Int J Mol Med 33: 589-596, 2014.
APA
Liu, Z., Zhang, J., Wu, L., Liu, J., & Zhang, M. (2014). Overexpression of GNAO1 correlates with poor prognosis in patients with gastric cancer and plays a role in gastric cancer cell proliferation and apoptosis. International Journal of Molecular Medicine, 33, 589-596. https://doi.org/10.3892/ijmm.2013.1598
MLA
Liu, Z., Zhang, J., Wu, L., Liu, J., Zhang, M."Overexpression of GNAO1 correlates with poor prognosis in patients with gastric cancer and plays a role in gastric cancer cell proliferation and apoptosis". International Journal of Molecular Medicine 33.3 (2014): 589-596.
Chicago
Liu, Z., Zhang, J., Wu, L., Liu, J., Zhang, M."Overexpression of GNAO1 correlates with poor prognosis in patients with gastric cancer and plays a role in gastric cancer cell proliferation and apoptosis". International Journal of Molecular Medicine 33, no. 3 (2014): 589-596. https://doi.org/10.3892/ijmm.2013.1598
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Z, Zhang J, Wu L, Liu J and Zhang M: Overexpression of GNAO1 correlates with poor prognosis in patients with gastric cancer and plays a role in gastric cancer cell proliferation and apoptosis. Int J Mol Med 33: 589-596, 2014.
APA
Liu, Z., Zhang, J., Wu, L., Liu, J., & Zhang, M. (2014). Overexpression of GNAO1 correlates with poor prognosis in patients with gastric cancer and plays a role in gastric cancer cell proliferation and apoptosis. International Journal of Molecular Medicine, 33, 589-596. https://doi.org/10.3892/ijmm.2013.1598
MLA
Liu, Z., Zhang, J., Wu, L., Liu, J., Zhang, M."Overexpression of GNAO1 correlates with poor prognosis in patients with gastric cancer and plays a role in gastric cancer cell proliferation and apoptosis". International Journal of Molecular Medicine 33.3 (2014): 589-596.
Chicago
Liu, Z., Zhang, J., Wu, L., Liu, J., Zhang, M."Overexpression of GNAO1 correlates with poor prognosis in patients with gastric cancer and plays a role in gastric cancer cell proliferation and apoptosis". International Journal of Molecular Medicine 33, no. 3 (2014): 589-596. https://doi.org/10.3892/ijmm.2013.1598
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