Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
2014-March Volume 33 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
2014-March Volume 33 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Arctiin induces an UVB protective effect in human dermal fibroblast cells through microRNA expression changes

  • Authors:
    • Ghang Tai Lee
    • Hwa Jun Cha
    • Kwang Sik Lee
    • Kun Kook Lee
    • Jin Tae Hong
    • Kyu Joong Ahn
    • In-Sook An
    • Sungkwan An
    • Seunghee Bae
  • View Affiliations / Copyright

    Affiliations: Songpa R&D Center, Coreana Cosmetics Co., Ltd., Cheonan, Chungcheongnam-do 330-833, Republic of Korea, Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea, College of Pharmacy and Medical Research Center, Chungbuk National University, Cheongju, Chungcheongbuk-do 361-763, Republic of Korea, Department of Dermatology, Konkuk University School of Medicine, Seoul 143-701, Republic of Korea
  • Pages: 640-648
    |
    Published online on: January 7, 2014
       https://doi.org/10.3892/ijmm.2014.1616
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Ultraviolet (UV) radiation induces severe alterations in the molecular and cellular components of normal human dermal fibroblast (NHDF) cells by disrupting many intracellular transduction cascades. Although UV responses have been well documented at the genome and proteome levels, UV protective effects have not been elucidated at these levels. The aim of the present study was to demonstrate that arctiin, a phytochemical isolated from the plant Arctium lappa, induced a protective effect against UVB radiation by changing microRNA (miRNA) expression profiles. Using flow cytometry, and water-soluble tetrazolium salt (WST-1)-based cell viability, wound healing, and DNA repair assays we showed that pretreatment with arctiin prior to UVB irradiation reduced UVB-induced apoptosis, cell migration defects, and DNA damage in NHDF cells. It was also found that arctiin‑induced UVB protection is associated with altered miRNA expression profiles. Bioinformatic analysis revealed that the deregulated miRNAs were functionally involved in mitogen-activated protein kinase (MAPK) signaling and cancer signaling pathways. The results suggest that arctiin acts as a UVB protective agent by altering specific miRNA expression in NHDF cells.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Sjerobabski-Masnec I and Situm M: Skin aging. Acta Clin Croat. 49:515–518. 2010.

2 

Wang B: Photoaging: a review of current concepts of pathogenesis. J Cutan Med Surg. 15(Suppl 1): S374–S377. 2011.PubMed/NCBI

3 

Wlaschek M, Tantcheva-Poór I, Naderi L, et al: Solar UV irradiation and dermal photoaging. J Photochem Photobiol B. 63:41–51. 2001. View Article : Google Scholar : PubMed/NCBI

4 

Diffey B: Climate change, ozone depletion and the impact on ultraviolet exposure of human skin. Phys Med Biol. 49:R1–R11. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Philips N, Auler S, Hugo R and Gonzalez S: Beneficial regulation of matrix metalloproteinases for skin health. Enzyme Res. 2011:4272852011. View Article : Google Scholar : PubMed/NCBI

6 

Muthusamy V and Piva TJ: The UV response of the skin: a review of the MAPK, NFkappaB and TNFalpha signal transduction pathways. Arch Dermatol Res. 302:5–17. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Travers JB, Edenberg HJ, Zhang Q, et al: Augmentation of UVB radiation-mediated early gene expression by the epidermal platelet-activating factor receptor. J Invest Dermatol. 128:455–460. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Casati P and Walbot V: Gene expression profiling in response to ultraviolet radiation in maize genotypes with varying flavonoid content. Plant Physiol. 132:1739–1754. 2003. View Article : Google Scholar : PubMed/NCBI

9 

Bartel DP: MicroRNAs: target recognition and regulatory functions. Cell. 136:215–233. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Griffiths-Jones S, Saini HK, van Dongen S and Enright AJ: miRBase: tools for microRNA genomics. Nucleic Acids Res. 36:D154–D158. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Li W, Zhou BR, Hua LJ, Guo Z and Luo D: Differential miRNA profile on photoaged primary human fibroblasts irradiated with ultraviolet A. Tumour Biol. Jul 7–2013.(Epub ahead of print).

12 

Lu C, Ding ZH and Zhou MJ: Mechanisms of ultraviolet B irradiation-induced injuries in 16HBE cells. Nan Fang Yi Ke Da Xue Xue Bao. 31:57–60. 2011.(In Chinese).

13 

Tan G, Shi Y and Wu ZH: MicroRNA-22 promotes cell survival upon UV radiation by repressing PTEN. Biochem Biophys Res Commun. 417:546–551. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Zhou BR, Guo XF, Zhang JA, et al: Elevated miR-34c-5p mediates dermal fibroblast senescence by ultraviolet irradiation. Int J Biol Sci. 9:743–752. 2013. View Article : Google Scholar : PubMed/NCBI

15 

An IS, An S, Kang SM, et al: Titrated extract of Centella asiatica provides a UVB protective effect by altering microRNA expression profiles in human dermal fibroblasts. Int J Mol Med. 30:1194–1202. 2012.

16 

An IS, An S, Park S, Lee SN and Bae S: Involvement of microRNAs in epigallocatechin gallate-mediated UVB protection in human dermal fibroblasts. Oncol Rep. 29:253–259. 2013.PubMed/NCBI

17 

Sun WJ, Sha ZF and Gao H: Determination of arctiin and arctigenin in Fructus Arctii by reverse-phase HPLC. Yao Xue Xue Bao. 27:549–551. 1992.

18 

Hirose M, Yamaguchi T, Lin C, et al: Effects of arctiin on PhIP-induced mammary, colon and pancreatic carcinogenesis in female Sprague-Dawley rats and MeIQx-induced hepatocarcinogenesis in male F344 rats. Cancer Lett. 155:79–88. 2000. View Article : Google Scholar : PubMed/NCBI

19 

Lee S, Shin S, Kim H, et al: Anti-inflammatory function of arctiin by inhibiting COX-2 expression via NF-κB pathways. J Inflamm (Lond). 8:162011.PubMed/NCBI

20 

Wu JG, Wu JZ, Sun LN, et al: Ameliorative effects of arctiin from Arctium lappa on experimental glomerulonephritis in rats. Phytomedicine. 16:1033–1041. 2009. View Article : Google Scholar

21 

Cui X, Zhang J, Du R, et al: HSF4 is involved in DNA damage repair through regulation of Rad51. Biochim Biophys Acta. 1822:1308–1315. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Maragkakis M, Reczko M, Simossis VA, et al: DIANA-microT web server: elucidating microRNA functions through target prediction. Nucleic Acids Res. 37:W273–W276. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Huang da W, Sherman BT and Lempicki RA: Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 4:44–57. 2009.PubMed/NCBI

24 

Lee CH, Wu SB, Hong CH, Yu HS and Wei YH: Molecular mechanisms of UV-induced apoptosis and its effects on skin residential cells: the implication in UV-based phototherapy. Int J Mol Sci. 14:6414–6435. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Singer AJ and Clark RA: Cutaneous wound healing. N Engl J Med. 341:738–746. 1999. View Article : Google Scholar : PubMed/NCBI

26 

Sinha RP and Häder DP: UV-induced DNA damage and repair: a review. Photochem Photobiol Sci. 1:225–236. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Syed DN, Khan MI, Shabbir M and Mukhtar H: MicroRNAs in skin response to UV radiation. Curr Drug Targets. 14:1128–1134. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Subramanyam D and Blelloch R: From microRNAs to targets: pathway discovery in cell fate transitions. Curr Opin Genet Dev. 21:498–503. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Hayashi K, Narutaki K, Nagaoka Y, Hayashi T and Uesato S: Therapeutic effect of arctiin and arctigenin in immunocompetent and immunocompromised mice infected with influenza A virus. Biol Pharm Bull. 33:1199–1205. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Matsuzaki Y, Koyama M, Hitomi T, et al: Arctiin induces cell growth inhibition through the down-regulation of cyclin D1 expression. Oncol Rep. 19:721–727. 2008.PubMed/NCBI

31 

Huang DM, Guh JH, Chueh SC and Teng CM: Modulation of anti-adhesion molecule MUC-1 is associated with arctiin-induced growth inhibition in PC-3 cells. Prostate. 59:260–267. 2004. View Article : Google Scholar : PubMed/NCBI

32 

Moritani S, Nomura M, Takeda Y and Miyamoto K: Cytotoxic components of bardanae fructus (goboshi). Biol Pharm Bull. 19:1515–1517. 1996. View Article : Google Scholar

33 

Yi R and Fuchs E: MicroRNA-mediated control in the skin. Cell Death Differ. 17:229–235. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Zhou BR, Xu Y, Permatasari F, et al: Characterization of the miRNA profile in UVB-irradiated normal human keratinocytes. Exp Dermatol. 21:317–319. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Bemis LT, Chen R, Amato CM, et al: MicroRNA-137 targets microphthalmia-associated transcription factor in melanoma cell lines. Cancer Res. 68:1362–1368. 2008. View Article : Google Scholar : PubMed/NCBI

36 

Hildebrand J, Rütze M, Walz N, et al: A comprehensive analysis of microRNA expression during human keratinocyte differentiation in vitro and in vivo. J Invest Dermatol. 131:20–29. 2011. View Article : Google Scholar : PubMed/NCBI

37 

Yang L, Ma Z, Wang D, Zhao W, Chen L and Wang G: MicroRNA-602 regulating tumor suppressive gene RASSF1A is overexpressed in hepatitis B virus-infected liver and hepatocellular carcinoma. Cancer Biol Ther. 9:803–808. 2010. View Article : Google Scholar : PubMed/NCBI

38 

Fernandes MS, Carneiro F, Oliveira C and Seruca R: Colorectal cancer and RASSF family--a special emphasis on RASSF1A. Int J Cancer. 132:251–258. 2013. View Article : Google Scholar : PubMed/NCBI

39 

Cao D, Chen Y, Tang Y, et al: Loss of RASSF1A expression in colorectal cancer and its association with K-ras status. Biomed Res Int. 2013:9767652013.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Lee GT, Cha HJ, Lee KS, Lee KK, Hong JT, Ahn KJ, An I, An S and Bae S: Arctiin induces an UVB protective effect in human dermal fibroblast cells through microRNA expression changes. Int J Mol Med 33: 640-648, 2014.
APA
Lee, G.T., Cha, H.J., Lee, K.S., Lee, K.K., Hong, J.T., Ahn, K.J. ... Bae, S. (2014). Arctiin induces an UVB protective effect in human dermal fibroblast cells through microRNA expression changes. International Journal of Molecular Medicine, 33, 640-648. https://doi.org/10.3892/ijmm.2014.1616
MLA
Lee, G. T., Cha, H. J., Lee, K. S., Lee, K. K., Hong, J. T., Ahn, K. J., An, I., An, S., Bae, S."Arctiin induces an UVB protective effect in human dermal fibroblast cells through microRNA expression changes". International Journal of Molecular Medicine 33.3 (2014): 640-648.
Chicago
Lee, G. T., Cha, H. J., Lee, K. S., Lee, K. K., Hong, J. T., Ahn, K. J., An, I., An, S., Bae, S."Arctiin induces an UVB protective effect in human dermal fibroblast cells through microRNA expression changes". International Journal of Molecular Medicine 33, no. 3 (2014): 640-648. https://doi.org/10.3892/ijmm.2014.1616
Copy and paste a formatted citation
x
Spandidos Publications style
Lee GT, Cha HJ, Lee KS, Lee KK, Hong JT, Ahn KJ, An I, An S and Bae S: Arctiin induces an UVB protective effect in human dermal fibroblast cells through microRNA expression changes. Int J Mol Med 33: 640-648, 2014.
APA
Lee, G.T., Cha, H.J., Lee, K.S., Lee, K.K., Hong, J.T., Ahn, K.J. ... Bae, S. (2014). Arctiin induces an UVB protective effect in human dermal fibroblast cells through microRNA expression changes. International Journal of Molecular Medicine, 33, 640-648. https://doi.org/10.3892/ijmm.2014.1616
MLA
Lee, G. T., Cha, H. J., Lee, K. S., Lee, K. K., Hong, J. T., Ahn, K. J., An, I., An, S., Bae, S."Arctiin induces an UVB protective effect in human dermal fibroblast cells through microRNA expression changes". International Journal of Molecular Medicine 33.3 (2014): 640-648.
Chicago
Lee, G. T., Cha, H. J., Lee, K. S., Lee, K. K., Hong, J. T., Ahn, K. J., An, I., An, S., Bae, S."Arctiin induces an UVB protective effect in human dermal fibroblast cells through microRNA expression changes". International Journal of Molecular Medicine 33, no. 3 (2014): 640-648. https://doi.org/10.3892/ijmm.2014.1616
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team