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International Journal of Molecular Medicine
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2014-March Volume 33 Issue 3

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Article

Role of Rho kinase in lysophosphatidic acid-induced altering of blood-brain barrier permeability

  • Authors:
    • Ying Yu
    • Jun Qin
    • Meizhen Liu
    • Qingyuan Ruan
    • Yiliang Li
    • Zhaohui Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Department of Orthopaedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China
  • Pages: 661-669
    |
    Published online on: January 7, 2014
       https://doi.org/10.3892/ijmm.2014.1618
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Abstract

Lysophosphatidic acid (LPA) the simplest of the water-soluble phospholipids, is produced by activated platelets, macrophage and endothelial cells. It also evokes various biological responses. When LPA concentrations reach high levels, brain injury, including stroke and intracerebral hemorrhage (ICH), occurs. Previous studies have shown that LPA is crucial in increasing blood-brain barrier (BBB) permeability, and the Rho/Rho kinase (ROCK) signaling pathway is involved in the regulation of endothelial permeability. However, the exact mechanism by which the Rho/ROCK pathway mediates BBB disruption induced by LPA remains to be determined. In the present study, we observed that LPA induced the increase of BBB permeability in the right striatum after 10 µl LPA (100 µM) was injected into the ipsilateral caudate nucleus of rats. The ROCK was involved in the expression of proteolytic enzymes, matrix metalloproteinase (MMP)-9 and urokinase‑type plasminogen activator (uPA), leading to LPA-induced BBB disruption. ROCK inhibitor (Y27632) markedly inhibited the expression of proteolytic enzymes induced by LPA as well as the BBB disruption after it was co-injected with LPA. Thus, results of the present study suggest that LPA increases BBB permeability, which may be due to the Rho/ROCK signaling pathway and the subsequent production of proteolytic enzymes MMP-9 and uPA.
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Copy and paste a formatted citation
Spandidos Publications style
Yu Y, Qin J, Liu M, Ruan Q, Li Y and Zhang Z: Role of Rho kinase in lysophosphatidic acid-induced altering of blood-brain barrier permeability. Int J Mol Med 33: 661-669, 2014.
APA
Yu, Y., Qin, J., Liu, M., Ruan, Q., Li, Y., & Zhang, Z. (2014). Role of Rho kinase in lysophosphatidic acid-induced altering of blood-brain barrier permeability. International Journal of Molecular Medicine, 33, 661-669. https://doi.org/10.3892/ijmm.2014.1618
MLA
Yu, Y., Qin, J., Liu, M., Ruan, Q., Li, Y., Zhang, Z."Role of Rho kinase in lysophosphatidic acid-induced altering of blood-brain barrier permeability". International Journal of Molecular Medicine 33.3 (2014): 661-669.
Chicago
Yu, Y., Qin, J., Liu, M., Ruan, Q., Li, Y., Zhang, Z."Role of Rho kinase in lysophosphatidic acid-induced altering of blood-brain barrier permeability". International Journal of Molecular Medicine 33, no. 3 (2014): 661-669. https://doi.org/10.3892/ijmm.2014.1618
Copy and paste a formatted citation
x
Spandidos Publications style
Yu Y, Qin J, Liu M, Ruan Q, Li Y and Zhang Z: Role of Rho kinase in lysophosphatidic acid-induced altering of blood-brain barrier permeability. Int J Mol Med 33: 661-669, 2014.
APA
Yu, Y., Qin, J., Liu, M., Ruan, Q., Li, Y., & Zhang, Z. (2014). Role of Rho kinase in lysophosphatidic acid-induced altering of blood-brain barrier permeability. International Journal of Molecular Medicine, 33, 661-669. https://doi.org/10.3892/ijmm.2014.1618
MLA
Yu, Y., Qin, J., Liu, M., Ruan, Q., Li, Y., Zhang, Z."Role of Rho kinase in lysophosphatidic acid-induced altering of blood-brain barrier permeability". International Journal of Molecular Medicine 33.3 (2014): 661-669.
Chicago
Yu, Y., Qin, J., Liu, M., Ruan, Q., Li, Y., Zhang, Z."Role of Rho kinase in lysophosphatidic acid-induced altering of blood-brain barrier permeability". International Journal of Molecular Medicine 33, no. 3 (2014): 661-669. https://doi.org/10.3892/ijmm.2014.1618
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