A canine model of Alzheimer's disease generated by overexpressing a mutated human amyloid precursor protein

  • Authors:
    • Geun-Shik Lee
    • Yeon Woo Jeong
    • Joung Joo Kim
    • Sun Woo Park
    • Kyeong Hee Ko
    • Mina Kang
    • Yu Kyung Kim
    • Eui-Man Jung
    • Changjong Moon
    • Sang Hwan Hyun
    • Kyu‑Chan Hwang
    • Nam-Hyung Kim
    • Taeyoung Shin
    • Eui-Bae Jeung
    • Woo Suk Hwang
  • View Affiliations

  • Published online on: January 28, 2014     https://doi.org/10.3892/ijmm.2014.1636
  • Pages: 1003-1012
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Abstract

Canines are considered the most authentic model for studying multifactorial human diseases, as these animals typically share a common environment with man. Somatic cell nuclear transfer (SCNT) technology along with genetic engineering of nuclear donor cells provides a unique opportunity for examining human diseases using transgenic canines. In the present study, we generated transgenic canines that overexpressed the human amyloid precursor protein (APP) gene containing well-characterized familial Alzheimer's disease (AD) mutations. We successfully obtained five out of six live puppies by SCNT. This was confirmed by observing the expression of green fluorescence protein in the body as a visual transgenic marker and the overexpression of the mutated APP gene in the brain. The transgenic canines developed AD-like symptoms, such as enlarged ventricles, an atrophied hippocampus, and β-amyloid plaques in the brain. Thus, the transgenic canines we created can serve as a novel animal model for studying human AD.
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2014-April
Volume 33 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Lee G, Jeong YW, Kim JJ, Park SW, Ko KH, Kang M, Kim YK, Jung E, Moon C, Hyun SH, Hyun SH, et al: A canine model of Alzheimer's disease generated by overexpressing a mutated human amyloid precursor protein. Int J Mol Med 33: 1003-1012, 2014
APA
Lee, G., Jeong, Y.W., Kim, J.J., Park, S.W., Ko, K.H., Kang, M. ... Hwang, W.S. (2014). A canine model of Alzheimer's disease generated by overexpressing a mutated human amyloid precursor protein. International Journal of Molecular Medicine, 33, 1003-1012. https://doi.org/10.3892/ijmm.2014.1636
MLA
Lee, G., Jeong, Y. W., Kim, J. J., Park, S. W., Ko, K. H., Kang, M., Kim, Y. K., Jung, E., Moon, C., Hyun, S. H., Hwang, K., Kim, N., Shin, T., Jeung, E., Hwang, W. S."A canine model of Alzheimer's disease generated by overexpressing a mutated human amyloid precursor protein". International Journal of Molecular Medicine 33.4 (2014): 1003-1012.
Chicago
Lee, G., Jeong, Y. W., Kim, J. J., Park, S. W., Ko, K. H., Kang, M., Kim, Y. K., Jung, E., Moon, C., Hyun, S. H., Hwang, K., Kim, N., Shin, T., Jeung, E., Hwang, W. S."A canine model of Alzheimer's disease generated by overexpressing a mutated human amyloid precursor protein". International Journal of Molecular Medicine 33, no. 4 (2014): 1003-1012. https://doi.org/10.3892/ijmm.2014.1636