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International Journal of Molecular Medicine
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2014-April Volume 33 Issue 4

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SPARC silencing inhibits the growth of acute myeloid leukemia transformed from myelodysplastic syndrome via induction of cell cycle arrest and apoptosis

  • Authors:
    • Qing Nian
    • Qing Xiao
    • Li Wang
    • Jing Luo
    • Li-Ping Chen
    • Ze-Song Yang
    • Lin Liu
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China
    Copyright: © Nian et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 856-862
    |
    Published online on: February 7, 2014
       https://doi.org/10.3892/ijmm.2014.1648
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Abstract

Secreted protein acidic and rich in cysteine (SPARC) plays key roles in erythropoiesis; haploinsufficiency of SPARC is implicated in the progression of the 5q- syndrome. However, the role of SPARC in other subtypes of myelodysplastic syndrome (MDS) is not fully understood, particularly in the del(5q) type with a complex karyotype, which has a high risk to transform into acute myeloid leukemia (AML). In the present study, we investigated the role of SPARC in the proliferation and apoptosis of SKM-1 cells, an acute myeloid leukemia cell line transformed from an MDS cell line. SKM-1 cells were infected with SPARC-RNAi-LV or NC-GFP-LV lentivirus. Apoptosis and cell cycle profiling were assessed by flow cytometry, and cell proliferation was evaluated by MTS assay. The mRNA and protein expression levels of SPARC, p53, caspase-3, caspase-9 and Fas were detected by RT-PCR, real-time PCR and western blot assay. The SPARC shRNA constructed by us led to a significant reduction in SPARC expression in SKM-1 cells. SPARC knockdown inhibited the proliferation of SKM-1 cells by inducing cell cycle arrest at the G1/G0 phase and apoptosis. SPARC knockdown elevated the expression of p53, caspase-9, caspase-3 and Fas at both the mRNA and protein levels. SPARC silencing inhibited the growth of AML transformed from MDS by activating p53-induced apoptosis and cell cycle arrest. These data indicate that SPARC acts as an oncogene in transformed MDS/AML and is a potential therapeutic target in MDS/AML.
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1 

Nimer SD: Myelodysplastic syndromes. Blood. 111:4841–4851. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Ghariani I, Braham N, Hassine M and Kortas M: Myelodysplastic syndrome classification. Ann Biol Clin. 71:139–144. 2013.(In French).

3 

Jädersten M and Hellström-Lindberg E: New clues to the molecular pathogenesis of myelodysplastic syndromes. Exp Cell Res. 316:1390–1396. 2010.PubMed/NCBI

4 

Davids MS and Steensma DP: The molecular pathogenesis of myelodysplastic syndromes. Cancer Biol Ther. 10:309–319. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Giagounidis AA, Germing U, Wainscoat JS, et al: The 5q- syndrome. Hematology. 9:271–277. 2004.

6 

Swaminathan SS, Oh DJ, Kang MH, et al: Secreted protein acidic and rich in cysteine (SPARC)-null mice exhibit more uniform outflow. Invest Ophthalmol Vis Sci. 54:2035–2047. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Rotllant J, Liu D, Yan YL, et al: Sparc (osteonectin) functions in morphogenesis of the pharyngeal skeleton and inner ear. Matrix Biol. 27:561–572. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Hsiao YH, Lien HC, Hwa HL, et al: SPARC (osteonectin) in breast tumors of different histologic types and its role in the outcome of invasive ductal carcinoma. Breast J. 16:305–308. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Wiese AH, Auer J, Lassmann S, et al: Identification of gene signatures for invasive colorectal tumor cells. Cancer Detect Prev. 31:282–295. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Said N, Frierson HF Jr, Chernauskas D, et al: The role of SPARC in the TRAMP model of prostate carcinogenesis and progression. Oncogene. 28:3487–3498. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Isler SG, Ludwig CU, Chiquet-Ehrismann R, et al: Evidence for transcriptional repression of SPARC-like 1, a gene downregulated in human lung tumors. Int J Oncol. 25:1073–1079. 2004.PubMed/NCBI

12 

Tohyama K: 5q- syndrome, MDS with isolated del(5q). Nihon Rinsho. 2:362–366. 2012.(In Japanese).

13 

Duong VH, Komrokji RS and List AF: Efficacy and safety of lenalidomide in patients with myelodysplastic syndrome with chromosome 5q deletion. Ther Adv Hematol. 3:105–116. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Usmani SZ, Sawyer J, Rosenthal A, et al: Risk factors for MDS and acute leukemia following total therapy 2 and 3 for multiple myeloma. Blood. 121:4753–4757. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Nakagawa T, Matozaki S, Murayama T, et al: Establishment of a leukaemic cell line from a patient with acquisition of chromosomal abnormalities during disease progression in myelodysplastic syndrome. Br J Haematol. 85:469–476. 1993. View Article : Google Scholar : PubMed/NCBI

16 

Nakagawa T and Matozaki S: The SKM-1 leukemic cell line established from a patient with progression to myelomonocytic leukemia in myelodysplastic syndrome (MDS) - contribution to better understanding of MDS. Leuk Lymphoma. 17:335–339. 1995. View Article : Google Scholar : PubMed/NCBI

17 

Kimura S, Kuramoto K, Homan J, et al: Antiproliferative and antitumor effects of azacitidine against the human myelodysplastic syndrome cell line SKM-1. Anticancer Res. 32:795–798. 2012.PubMed/NCBI

18 

Barlow JL, Drynan LF, Hewett DR, et al: A p53-dependent mechanism underlies macrocytic anemia in a mouse model of human 5q- syndrome. Nat Med. 16:59–66. 2010. View Article : Google Scholar

19 

Liebermann DA, Hoffman B and Vesely D: p53 induced growth arrest versus apoptosis and its modulation by survival cytokines. Cell Cycle. 6:166–170. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Yiu GK, Chan WY, Ng SW, et al: SPARC (secreted protein acidic and rich in cysteine) induces apoptosis in ovarian cancer cells. Am J Pathol. 159:609–622. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Seno T, Harada H, Kohno S, et al: Downregulation of SPARC expression inhibits cell migration and invasion in malignant gliomas. Int J Oncol. 34:707–715. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Yin J, Chen G, Liu Y, et al: Downregulation of SPARC expression decreases gastric cancer cellular invasion and survival. J Exp Clin Cancer Res. 29:592010. View Article : Google Scholar : PubMed/NCBI

23 

Horie K, Tsuchihara M and Nakatsura T: Silencing of secreted protein acidic and rich in cysteine inhibits the growth of human melanoma cells with G arrest induction. Cancer Sci. 101:913–919. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Khalil H, Peltzer N, Walicki J, et al: Caspase-3 protects stressed organs against cell death. Mol Cell Biol. 32:4523–4533. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Brentnall M, Rodriguez-Menocal L, De Guevara RL, et al: Caspase-9, caspase-3 and caspase-7 have distinct roles during intrinsic apoptosis. BMC Cell Biol. 14:322013. View Article : Google Scholar : PubMed/NCBI

26 

Li Q, Wang Y, Wang Y, et al: Distinct different sensitivity of Treg and Th17 cells to Fas-mediated apoptosis signaling in patients with acute coronary syndrome. Int J Clin Exp Pathol. 6:297–307. 2013.PubMed/NCBI

27 

Wang L, Fidler C, Nadig N, et al: Genome-wide analysis of copy number changes and loss of heterozygosity in myelodysplastic syndrome with del(5q) using high-density single nucleotide polymorphism arrays. Haematologica. 93:994–1000. 2008. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Nian Q, Xiao Q, Wang L, Luo J, Chen L, Yang Z and Liu L: SPARC silencing inhibits the growth of acute myeloid leukemia transformed from myelodysplastic syndrome via induction of cell cycle arrest and apoptosis. Int J Mol Med 33: 856-862, 2014.
APA
Nian, Q., Xiao, Q., Wang, L., Luo, J., Chen, L., Yang, Z., & Liu, L. (2014). SPARC silencing inhibits the growth of acute myeloid leukemia transformed from myelodysplastic syndrome via induction of cell cycle arrest and apoptosis. International Journal of Molecular Medicine, 33, 856-862. https://doi.org/10.3892/ijmm.2014.1648
MLA
Nian, Q., Xiao, Q., Wang, L., Luo, J., Chen, L., Yang, Z., Liu, L."SPARC silencing inhibits the growth of acute myeloid leukemia transformed from myelodysplastic syndrome via induction of cell cycle arrest and apoptosis". International Journal of Molecular Medicine 33.4 (2014): 856-862.
Chicago
Nian, Q., Xiao, Q., Wang, L., Luo, J., Chen, L., Yang, Z., Liu, L."SPARC silencing inhibits the growth of acute myeloid leukemia transformed from myelodysplastic syndrome via induction of cell cycle arrest and apoptosis". International Journal of Molecular Medicine 33, no. 4 (2014): 856-862. https://doi.org/10.3892/ijmm.2014.1648
Copy and paste a formatted citation
x
Spandidos Publications style
Nian Q, Xiao Q, Wang L, Luo J, Chen L, Yang Z and Liu L: SPARC silencing inhibits the growth of acute myeloid leukemia transformed from myelodysplastic syndrome via induction of cell cycle arrest and apoptosis. Int J Mol Med 33: 856-862, 2014.
APA
Nian, Q., Xiao, Q., Wang, L., Luo, J., Chen, L., Yang, Z., & Liu, L. (2014). SPARC silencing inhibits the growth of acute myeloid leukemia transformed from myelodysplastic syndrome via induction of cell cycle arrest and apoptosis. International Journal of Molecular Medicine, 33, 856-862. https://doi.org/10.3892/ijmm.2014.1648
MLA
Nian, Q., Xiao, Q., Wang, L., Luo, J., Chen, L., Yang, Z., Liu, L."SPARC silencing inhibits the growth of acute myeloid leukemia transformed from myelodysplastic syndrome via induction of cell cycle arrest and apoptosis". International Journal of Molecular Medicine 33.4 (2014): 856-862.
Chicago
Nian, Q., Xiao, Q., Wang, L., Luo, J., Chen, L., Yang, Z., Liu, L."SPARC silencing inhibits the growth of acute myeloid leukemia transformed from myelodysplastic syndrome via induction of cell cycle arrest and apoptosis". International Journal of Molecular Medicine 33, no. 4 (2014): 856-862. https://doi.org/10.3892/ijmm.2014.1648
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