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Article

Farnesol induces apoptosis of DU145 prostate cancer cells through the PI3K/Akt and MAPK pathways

  • Authors:
    • Jin Soo Park
    • Jung Ki Kwon
    • Hye Ri Kim
    • Hyeong Jin Kim
    • Byeong Soo Kim
    • Ji Youn Jung
  • View Affiliations / Copyright

    Affiliations: Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Chungnam 340702, Republic of Korea
  • Pages: 1169-1176
    |
    Published online on: February 27, 2014
       https://doi.org/10.3892/ijmm.2014.1679
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Abstract

The aim of this study was to investigate the effect of farnesol on the induction of apoptosis in DU145 prostate cancer cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay showed that cell proliferation decreased significantly in a dose- and time-dependent manner. 4',6-Diamidino-2-phenylindole staining showed that chromatin condensation in cells treated with 60 µM of farnesol was markedly higher than in the control groups. Farnesol increased the expression of p53, p-c-Jun N-terminal kinase, cleaved-caspase-3, Bax, and cleaved-caspase-9, but decreased the expression of p-phosphatidylinositol-3-kinase (PI3K), p-Akt, p-p38, Bcl-2, and p-extracellular signal-regulated protein kinase, in a dose-dependent manner. The apoptotic cell ratio increased in a dose-dependent manner. The tumor growth inhibitory effect of farnesol was investigated in a mouse model. Compared to the control group, tumor volume decreased significantly in the group administered 50 mg/kg farnesol. Apoptosis was frequently detected in this same group by terminal deoxynucleotidyl transferase dUTP nick-end labeling assay. The results indicated that farnesol induced apoptosis of DU145 prostate cancer cells through the PI3K/Akt and mitogen-activated protein kinase signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Park JS, Kwon JK, Kim HR, Kim HJ, Kim BS and Jung JY: Farnesol induces apoptosis of DU145 prostate cancer cells through the PI3K/Akt and MAPK pathways. Int J Mol Med 33: 1169-1176, 2014.
APA
Park, J.S., Kwon, J.K., Kim, H.R., Kim, H.J., Kim, B.S., & Jung, J.Y. (2014). Farnesol induces apoptosis of DU145 prostate cancer cells through the PI3K/Akt and MAPK pathways. International Journal of Molecular Medicine, 33, 1169-1176. https://doi.org/10.3892/ijmm.2014.1679
MLA
Park, J. S., Kwon, J. K., Kim, H. R., Kim, H. J., Kim, B. S., Jung, J. Y."Farnesol induces apoptosis of DU145 prostate cancer cells through the PI3K/Akt and MAPK pathways". International Journal of Molecular Medicine 33.5 (2014): 1169-1176.
Chicago
Park, J. S., Kwon, J. K., Kim, H. R., Kim, H. J., Kim, B. S., Jung, J. Y."Farnesol induces apoptosis of DU145 prostate cancer cells through the PI3K/Akt and MAPK pathways". International Journal of Molecular Medicine 33, no. 5 (2014): 1169-1176. https://doi.org/10.3892/ijmm.2014.1679
Copy and paste a formatted citation
x
Spandidos Publications style
Park JS, Kwon JK, Kim HR, Kim HJ, Kim BS and Jung JY: Farnesol induces apoptosis of DU145 prostate cancer cells through the PI3K/Akt and MAPK pathways. Int J Mol Med 33: 1169-1176, 2014.
APA
Park, J.S., Kwon, J.K., Kim, H.R., Kim, H.J., Kim, B.S., & Jung, J.Y. (2014). Farnesol induces apoptosis of DU145 prostate cancer cells through the PI3K/Akt and MAPK pathways. International Journal of Molecular Medicine, 33, 1169-1176. https://doi.org/10.3892/ijmm.2014.1679
MLA
Park, J. S., Kwon, J. K., Kim, H. R., Kim, H. J., Kim, B. S., Jung, J. Y."Farnesol induces apoptosis of DU145 prostate cancer cells through the PI3K/Akt and MAPK pathways". International Journal of Molecular Medicine 33.5 (2014): 1169-1176.
Chicago
Park, J. S., Kwon, J. K., Kim, H. R., Kim, H. J., Kim, B. S., Jung, J. Y."Farnesol induces apoptosis of DU145 prostate cancer cells through the PI3K/Akt and MAPK pathways". International Journal of Molecular Medicine 33, no. 5 (2014): 1169-1176. https://doi.org/10.3892/ijmm.2014.1679
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