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International Journal of Molecular Medicine
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Article

The effect of zipper‑interacting protein kinase on high glucose‑stimulated human aortic smooth muscle cells

  • Authors:
    • Zi Ye
    • Wei Wu
    • Min He
    • Mancheong Leong
    • Renming Hu
    • Yiming Li
    • Shuo Zhang
  • View Affiliations / Copyright

    Affiliations: Institute of Endocrinology and Diabetology, Huashan Hospital, School of Medicine, Fudan University, Shanghai 200040, P.R. China, Department of Endocrinology, City University of Macau, Macau 000853, P.R. China
  • Pages: 1305-1311
    |
    Published online on: March 14, 2014
       https://doi.org/10.3892/ijmm.2014.1697
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Abstract

Biologic abnormalities in vascular smooth muscle cells (VSMC) may perform a crucial role in the pathogenesis of diabetic vascular disease. The principal aim of this study was to determine the effects of zipper‑interacting protein kinase (ZIPK) on human aortic smooth muscle cells (HASMCs) stimulated by high glucose (HG). To elucidate the role of ZIPK in HG‑treated HASMCs, we overexpressed ZIPK by lentivirus infection and knocked down ZIPK by gene deletion using ZIPK shRNA. Flow cytometry and Cell Counting kit‑8 (CCK‑8) were separately used to analyze cell apoptosis and proliferation. Migratory activity was examined using transwell migration chamber assays. The results showed that ZIPK overexpression inhibited cell growth and migration, enhanced cell apoptosis, and reversed cell cycle disturbance by regulating the related proteins of cellular physiological process, such as human cell division cycle 14A phosphatase (Hcdc14A) and intercellular adhesion molecule 1 (ICAM‑1). In conclusion, the results suggested that ZIPK plays a role in HG‑treated HASMCs, indicating ZIPK is a potential therapeutic target for the treatment of diabetic vascular complications.
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Copy and paste a formatted citation
Spandidos Publications style
Ye Z, Wu W, He M, Leong M, Hu R, Li Y and Zhang S: The effect of zipper‑interacting protein kinase on high glucose‑stimulated human aortic smooth muscle cells. Int J Mol Med 33: 1305-1311, 2014.
APA
Ye, Z., Wu, W., He, M., Leong, M., Hu, R., Li, Y., & Zhang, S. (2014). The effect of zipper‑interacting protein kinase on high glucose‑stimulated human aortic smooth muscle cells. International Journal of Molecular Medicine, 33, 1305-1311. https://doi.org/10.3892/ijmm.2014.1697
MLA
Ye, Z., Wu, W., He, M., Leong, M., Hu, R., Li, Y., Zhang, S."The effect of zipper‑interacting protein kinase on high glucose‑stimulated human aortic smooth muscle cells". International Journal of Molecular Medicine 33.5 (2014): 1305-1311.
Chicago
Ye, Z., Wu, W., He, M., Leong, M., Hu, R., Li, Y., Zhang, S."The effect of zipper‑interacting protein kinase on high glucose‑stimulated human aortic smooth muscle cells". International Journal of Molecular Medicine 33, no. 5 (2014): 1305-1311. https://doi.org/10.3892/ijmm.2014.1697
Copy and paste a formatted citation
x
Spandidos Publications style
Ye Z, Wu W, He M, Leong M, Hu R, Li Y and Zhang S: The effect of zipper‑interacting protein kinase on high glucose‑stimulated human aortic smooth muscle cells. Int J Mol Med 33: 1305-1311, 2014.
APA
Ye, Z., Wu, W., He, M., Leong, M., Hu, R., Li, Y., & Zhang, S. (2014). The effect of zipper‑interacting protein kinase on high glucose‑stimulated human aortic smooth muscle cells. International Journal of Molecular Medicine, 33, 1305-1311. https://doi.org/10.3892/ijmm.2014.1697
MLA
Ye, Z., Wu, W., He, M., Leong, M., Hu, R., Li, Y., Zhang, S."The effect of zipper‑interacting protein kinase on high glucose‑stimulated human aortic smooth muscle cells". International Journal of Molecular Medicine 33.5 (2014): 1305-1311.
Chicago
Ye, Z., Wu, W., He, M., Leong, M., Hu, R., Li, Y., Zhang, S."The effect of zipper‑interacting protein kinase on high glucose‑stimulated human aortic smooth muscle cells". International Journal of Molecular Medicine 33, no. 5 (2014): 1305-1311. https://doi.org/10.3892/ijmm.2014.1697
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