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The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA

  • Authors:
    • Liyuan Liu
    • Yingxiong Wang
    • Qiubo Yu
  • View Affiliations / Copyright

    Affiliations: College of Basic Medicine, Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China, Molecular Medical Laboratory, Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1089-1096
    |
    Published online on: March 17, 2014
       https://doi.org/10.3892/ijmm.2014.1701
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Abstract

The aim of this study was to investigate whether the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway affects the implantation of mouse embryos by regulating the expression of RhoA. The expression of PI3K, Akt, phosphorylated (p-)Akt, phosphatase and tensin homolog (PTEN) and RhoA in the uterus of mice on day 5 of pregnancy (D5) and in pseudopregnant mice was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunohistochemistry and western blot analysis. A functional analysis of these genes was also performed by the intrauterine injection with the PI3K inhibitor, LY294002, on day 2 of pregnancy (D2). The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression. The expression levels of the above genes in the pseudopregnant group and in the group injected with the PI3K/Akt inhibitor, LY294002, were markedly lower than those in the pregnant group. Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS). These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.
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Copy and paste a formatted citation
Spandidos Publications style
Liu L, Wang Y and Yu Q: The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA. Int J Mol Med 33: 1089-1096, 2014.
APA
Liu, L., Wang, Y., & Yu, Q. (2014). The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA. International Journal of Molecular Medicine, 33, 1089-1096. https://doi.org/10.3892/ijmm.2014.1701
MLA
Liu, L., Wang, Y., Yu, Q."The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA". International Journal of Molecular Medicine 33.5 (2014): 1089-1096.
Chicago
Liu, L., Wang, Y., Yu, Q."The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA". International Journal of Molecular Medicine 33, no. 5 (2014): 1089-1096. https://doi.org/10.3892/ijmm.2014.1701
Copy and paste a formatted citation
x
Spandidos Publications style
Liu L, Wang Y and Yu Q: The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA. Int J Mol Med 33: 1089-1096, 2014.
APA
Liu, L., Wang, Y., & Yu, Q. (2014). The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA. International Journal of Molecular Medicine, 33, 1089-1096. https://doi.org/10.3892/ijmm.2014.1701
MLA
Liu, L., Wang, Y., Yu, Q."The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA". International Journal of Molecular Medicine 33.5 (2014): 1089-1096.
Chicago
Liu, L., Wang, Y., Yu, Q."The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA". International Journal of Molecular Medicine 33, no. 5 (2014): 1089-1096. https://doi.org/10.3892/ijmm.2014.1701
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