Electroacupuncture promotes neural cell proliferation in vivo through activation of the ERK1/2 signaling pathway

  • Authors:
    • Jia Huang
    • Xiaoqian Ye
    • Yongmei You
    • Weilin Liu
    • Yanling Gao
    • Shanli Yang
    • Jun Peng
    • Zhenfeng Hong
    • Jing Tao
    • Lidian Chen
  • View Affiliations

  • Published online on: March 18, 2014     https://doi.org/10.3892/ijmm.2014.1702
  • Pages: 1547-1553
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Abstract

The aim of this study was to investigate the effect of electroacupuncture (EA) on cell proliferation and its molecular mechanisms. Sixty rats were randomly divided into 5 groups: sham operation control (SC), ischemia control (IC), EA, EA and DMSO injection (ED), EA and U0126 injection (EU). All the groups, with the exception of SC, underwent middle cerebral artery occlusion (MCAO), and DMSO or U0126 was injected into the rat in the ED or EU group 30 min prior to MCAO. Cell proliferation was evaluated by proliferating cell nuclear antigen (PCNA) immunostaining. The changes of cell cycle proteins (cyclin D1, CDK4, cyclin E, CDK2, p21 and p27) and the ERK1/2 pathway activation were examined by RT-PCR and western blot analysis. The results showed that the positive cell numbers of PCNA immunostaining in the EA and ED groups were more than those in the IC group (P<0.05). The mRNA and protein levels of p21 or p27 were obviously increased, however, the mRNA and protein levels of cyclin D1, CDK4, cyclin E and CDK2 were reduced in the IC and EU groups. The findings suggested that EA activates the ERK1/2 signaling pathway to protect brain injury during cerebral ischemia. However, this positive effect of EA can be blocked by U0126.
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June-2014
Volume 33 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Huang J, Ye X, You Y, Liu W, Gao Y, Yang S, Peng J, Hong Z, Tao J, Chen L, Chen L, et al: Electroacupuncture promotes neural cell proliferation in vivo through activation of the ERK1/2 signaling pathway. Int J Mol Med 33: 1547-1553, 2014
APA
Huang, J., Ye, X., You, Y., Liu, W., Gao, Y., Yang, S. ... Chen, L. (2014). Electroacupuncture promotes neural cell proliferation in vivo through activation of the ERK1/2 signaling pathway. International Journal of Molecular Medicine, 33, 1547-1553. https://doi.org/10.3892/ijmm.2014.1702
MLA
Huang, J., Ye, X., You, Y., Liu, W., Gao, Y., Yang, S., Peng, J., Hong, Z., Tao, J., Chen, L."Electroacupuncture promotes neural cell proliferation in vivo through activation of the ERK1/2 signaling pathway". International Journal of Molecular Medicine 33.6 (2014): 1547-1553.
Chicago
Huang, J., Ye, X., You, Y., Liu, W., Gao, Y., Yang, S., Peng, J., Hong, Z., Tao, J., Chen, L."Electroacupuncture promotes neural cell proliferation in vivo through activation of the ERK1/2 signaling pathway". International Journal of Molecular Medicine 33, no. 6 (2014): 1547-1553. https://doi.org/10.3892/ijmm.2014.1702