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International Journal of Molecular Medicine
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Article

Quercetin induces apoptosis by inhibiting MAPKs and TRPM7 channels in AGS cells

  • Authors:
    • Min Chul Kim
    • Hee Jung Lee
    • Bora Lim
    • Ki-Tae Ha
    • Sung Young Kim
    • Insuk So
    • Byung Joo Kim
  • View Affiliations / Copyright

    Affiliations: Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan 626-870, Republic of Korea, Division of Applied Medicine, Pusan National University School of Korean Medicine, Yangsan 626-870, Republic of Korea, Department of Physiology, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea
  • Pages: 1657-1663
    |
    Published online on: March 18, 2014
       https://doi.org/10.3892/ijmm.2014.1704
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Abstract

The worldwide incidence and mortality rate of gastric cancer remain high, and thus, novel treatment concepts are required. Quercetin, a bioflavonoid, has been proposed to have anti-cancer properties. The aim of this study was to determine the nature of the apoptotic mechanisms responsible for the effects of quercetin on AGS cells (a commonly used human gastric adenocarcinoma cell line). AGS cell viability was assessed by MTT assay and flow cytometric analysis, mitochondrial membrane depolarization was assessed, and caspase-3 was used to determine the involvement of apoptosis. Whole-cell configuration patch-clamp experiments were used to regulate the transient receptor potential melastatin (TRPM)7 channels. To investigate the signaling pathway of quercetin-induced apoptosis in the AGS cells, western blot analysis and MTT assay were performed. Quercetin was found to induce the apoptosis of these cells, and this apoptosis was inhibited by SB203580 (a p38 kinase inhibitor), SP600125 (a JNK inhibitor) and PD98059 (an ERK inhibitor). In addition, quercetin inhibited TRPM7 currents in the AGS cells and in human embryo kidney (HEK)293 cells which overexpress TRPM7 channels. Furthermore, treatment with quercetin increased the apoptosis of HEK293 cells, which overexpress TRPM7, indicating that the upregulation of TRPM7 channels underlies quercetin-induced cell death. These results suggest that quercetin plays an important pathophysiological role in AGS cells through mitogen‑activated protein kinase (MAPK) signaling pathways and TRPM7 channels, and that quercetin has potential as a pharmacological agent for the treatment of gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Kim MC, Lee HJ, Lim B, Ha K, Kim SY, So I and Kim BJ: Quercetin induces apoptosis by inhibiting MAPKs and TRPM7 channels in AGS cells. Int J Mol Med 33: 1657-1663, 2014.
APA
Kim, M.C., Lee, H.J., Lim, B., Ha, K., Kim, S.Y., So, I., & Kim, B.J. (2014). Quercetin induces apoptosis by inhibiting MAPKs and TRPM7 channels in AGS cells. International Journal of Molecular Medicine, 33, 1657-1663. https://doi.org/10.3892/ijmm.2014.1704
MLA
Kim, M. C., Lee, H. J., Lim, B., Ha, K., Kim, S. Y., So, I., Kim, B. J."Quercetin induces apoptosis by inhibiting MAPKs and TRPM7 channels in AGS cells". International Journal of Molecular Medicine 33.6 (2014): 1657-1663.
Chicago
Kim, M. C., Lee, H. J., Lim, B., Ha, K., Kim, S. Y., So, I., Kim, B. J."Quercetin induces apoptosis by inhibiting MAPKs and TRPM7 channels in AGS cells". International Journal of Molecular Medicine 33, no. 6 (2014): 1657-1663. https://doi.org/10.3892/ijmm.2014.1704
Copy and paste a formatted citation
x
Spandidos Publications style
Kim MC, Lee HJ, Lim B, Ha K, Kim SY, So I and Kim BJ: Quercetin induces apoptosis by inhibiting MAPKs and TRPM7 channels in AGS cells. Int J Mol Med 33: 1657-1663, 2014.
APA
Kim, M.C., Lee, H.J., Lim, B., Ha, K., Kim, S.Y., So, I., & Kim, B.J. (2014). Quercetin induces apoptosis by inhibiting MAPKs and TRPM7 channels in AGS cells. International Journal of Molecular Medicine, 33, 1657-1663. https://doi.org/10.3892/ijmm.2014.1704
MLA
Kim, M. C., Lee, H. J., Lim, B., Ha, K., Kim, S. Y., So, I., Kim, B. J."Quercetin induces apoptosis by inhibiting MAPKs and TRPM7 channels in AGS cells". International Journal of Molecular Medicine 33.6 (2014): 1657-1663.
Chicago
Kim, M. C., Lee, H. J., Lim, B., Ha, K., Kim, S. Y., So, I., Kim, B. J."Quercetin induces apoptosis by inhibiting MAPKs and TRPM7 channels in AGS cells". International Journal of Molecular Medicine 33, no. 6 (2014): 1657-1663. https://doi.org/10.3892/ijmm.2014.1704
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