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Reduced hTERT protein levels are associated with DNA aneuploidy in the colonic mucosa of patients suffering from longstanding ulcerative colitis

  • Authors:
    • Mariann Friis-Ottessen
    • Paula M. De Angelis
    • Aasa R. Schjølberg
    • Solveig N. Andersen
    • Ole Petter F. Clausen
  • View Affiliations / Copyright

    Affiliations: Division of Diagnostics and Intervention, Department of Pathology, Oslo University Hospital, Rikshospitalet, 0424 Oslo, Norway, Department of Pathology, University of Oslo, 0424 Oslo, Norway, Department of Pathology, Akershus University Hospital, Division of Medicine and Laboratory Sciences, University of Oslo, 1474 Nordbyhagen, Norway
    Copyright: © Friis-Ottessen et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1477-1483
    |
    Published online on: March 20, 2014
       https://doi.org/10.3892/ijmm.2014.1708
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Abstract

Longstanding ulcerative colitis (UC) is a disease of chronic inflammation of the colon. It is associated with the development of colorectal cancer through a multistep process including increasing degrees of dysplasia and DNA-ploidy changes. However, not all UC patients will develop these characteristics even during lifelong disease, and patients may therefore be divided into progressors who develop dysplasia or cancer, and non-progressors who do not exhibit such changes. In the present study, the amount of hTERT, the catalytic subunit of the enzyme telomerase, was estimated by using peroxidase immunohistochemistry (IHC) in a set of progressor and non-progressor UC colectomies. The protein levels in the colonic mucosa of the progressors and non‑progressors were compared, and further comparisons between different categories of dysplastic development and to DNA-ploidy status within the progressors were made. Levels of hTERT were elevated in the colonic mucosa of the progressors and non-progressors when compared to non-UC control samples, but no difference was observed between the hTERT levels in the mucosa of progressors and non-progressors. The levels of hTERT associated with levels of Ki67 to a significant degree within the non-progressors. hTERT expression in lesions with DNA-aneuploidy were decreased as compared to diploid lesions, when stratified for different classes of colonic morphology. Our results indicate an association between hTERT protein expression and aneuploidy in UC-progressor colons, and also a possible protective mechanism in the association between hTERT and Ki67, against development of malignant features within the mucosa of a UC-colon.
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Copy and paste a formatted citation
Spandidos Publications style
Friis-Ottessen M, De Angelis PM, Schjølberg AR, Andersen SN and Clausen OP: Reduced hTERT protein levels are associated with DNA aneuploidy in the colonic mucosa of patients suffering from longstanding ulcerative colitis. Int J Mol Med 33: 1477-1483, 2014.
APA
Friis-Ottessen, M., De Angelis, P.M., Schjølberg, A.R., Andersen, S.N., & Clausen, O.P. (2014). Reduced hTERT protein levels are associated with DNA aneuploidy in the colonic mucosa of patients suffering from longstanding ulcerative colitis. International Journal of Molecular Medicine, 33, 1477-1483. https://doi.org/10.3892/ijmm.2014.1708
MLA
Friis-Ottessen, M., De Angelis, P. M., Schjølberg, A. R., Andersen, S. N., Clausen, O. P."Reduced hTERT protein levels are associated with DNA aneuploidy in the colonic mucosa of patients suffering from longstanding ulcerative colitis". International Journal of Molecular Medicine 33.6 (2014): 1477-1483.
Chicago
Friis-Ottessen, M., De Angelis, P. M., Schjølberg, A. R., Andersen, S. N., Clausen, O. P."Reduced hTERT protein levels are associated with DNA aneuploidy in the colonic mucosa of patients suffering from longstanding ulcerative colitis". International Journal of Molecular Medicine 33, no. 6 (2014): 1477-1483. https://doi.org/10.3892/ijmm.2014.1708
Copy and paste a formatted citation
x
Spandidos Publications style
Friis-Ottessen M, De Angelis PM, Schjølberg AR, Andersen SN and Clausen OP: Reduced hTERT protein levels are associated with DNA aneuploidy in the colonic mucosa of patients suffering from longstanding ulcerative colitis. Int J Mol Med 33: 1477-1483, 2014.
APA
Friis-Ottessen, M., De Angelis, P.M., Schjølberg, A.R., Andersen, S.N., & Clausen, O.P. (2014). Reduced hTERT protein levels are associated with DNA aneuploidy in the colonic mucosa of patients suffering from longstanding ulcerative colitis. International Journal of Molecular Medicine, 33, 1477-1483. https://doi.org/10.3892/ijmm.2014.1708
MLA
Friis-Ottessen, M., De Angelis, P. M., Schjølberg, A. R., Andersen, S. N., Clausen, O. P."Reduced hTERT protein levels are associated with DNA aneuploidy in the colonic mucosa of patients suffering from longstanding ulcerative colitis". International Journal of Molecular Medicine 33.6 (2014): 1477-1483.
Chicago
Friis-Ottessen, M., De Angelis, P. M., Schjølberg, A. R., Andersen, S. N., Clausen, O. P."Reduced hTERT protein levels are associated with DNA aneuploidy in the colonic mucosa of patients suffering from longstanding ulcerative colitis". International Journal of Molecular Medicine 33, no. 6 (2014): 1477-1483. https://doi.org/10.3892/ijmm.2014.1708
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