Open Access

TNF-α promotes gallbladder cancer cell growth and invasion through autocrine mechanisms

  • Authors:
    • Guangwei Zhu
    • Qiang Du
    • Xiaoqian Wang
    • Nanhong Tang
    • Feifei She
    • Yanling Chen
  • View Affiliations

  • Published online on: March 24, 2014     https://doi.org/10.3892/ijmm.2014.1711
  • Pages: 1431-1440
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Tumor necrosis factor-α (TNF-α) has been suggested to be a putative tumor promoter gene, and autocrine of TNF-α expression has been found in colon cancer and ovarian cancer. As the role of autocrine TNF-α in human gallbladder cancer has not yet been elucidated, the present study examined the expression of TNF-α in gallbladder cancer-derived cell lines. Based on the data, TNF-α mRNA and TNF-α protein expression differed significantly different between the cell lines. In addition, using siRNA targeting TNF-α, the vector, pGPU-GFP-siTNF-α, was constructed and then transfected into the SGC-996 cells (gallbladder cancer cell line) which express high levels of endogenous TNF-α. In vitro experiments indicated that the silencing of TNF-α in the SGC-996 cells significantly suppressed proliferation and invasion. However, apoptosis was not induced by the silencing of TNF-α. Furthermore, we traced the mechanisms underlying these effects and found that the silencing of TNF-α affected the TNF-α-AKT-NF-κB-Bcl-2 pathway in the SGC-996 cells. Our data provide evidence that autocrine TNF-α plays a role as a tumor promoter gene in gallbladder cancer cells, possibly by promoting proliferation and invasion through autocrine mechanisms.
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June-2014
Volume 33 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
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Spandidos Publications style
Zhu G, Du Q, Wang X, Tang N, She F and Chen Y: TNF-α promotes gallbladder cancer cell growth and invasion through autocrine mechanisms. Int J Mol Med 33: 1431-1440, 2014.
APA
Zhu, G., Du, Q., Wang, X., Tang, N., She, F., & Chen, Y. (2014). TNF-α promotes gallbladder cancer cell growth and invasion through autocrine mechanisms. International Journal of Molecular Medicine, 33, 1431-1440. https://doi.org/10.3892/ijmm.2014.1711
MLA
Zhu, G., Du, Q., Wang, X., Tang, N., She, F., Chen, Y."TNF-α promotes gallbladder cancer cell growth and invasion through autocrine mechanisms". International Journal of Molecular Medicine 33.6 (2014): 1431-1440.
Chicago
Zhu, G., Du, Q., Wang, X., Tang, N., She, F., Chen, Y."TNF-α promotes gallbladder cancer cell growth and invasion through autocrine mechanisms". International Journal of Molecular Medicine 33, no. 6 (2014): 1431-1440. https://doi.org/10.3892/ijmm.2014.1711