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International Journal of Molecular Medicine
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Article

A microRNA-27a mimic sensitizes human oral squamous cell carcinoma HSC-4 cells to hyperthermia through downregulation of Hsp110 and Hsp90

  • Authors:
    • Ayako Kariya
    • Yukihiro Furusawa
    • Tatsuya Yunoki
    • Takashi Kondo
    • Yoshiaki Tabuchi
  • View Affiliations / Copyright

    Affiliations: Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan , Division of Molecular Genetics Research, Life Science Research Center, University of Toyama, Toyama 930-0194, Japan
  • Pages: 334-340
    |
    Published online on: April 25, 2014
       https://doi.org/10.3892/ijmm.2014.1758
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Abstract

Hyperthermia (HT) is an important modality in cancer treatment; however, the acquisition of thermal resistance in cancer cells due to the elevation of heat shock proteins (HSPs) makes HT less effective. Accumulating evidence suggests that microRNAs (miRNAs) play an important role in regulating cellular stress sensitivities, such as drug sensitivity and radio-sensitivity, in cancer cells. However, few studies have investigated the involvement of miRNAs in thermal sensitivity. The aim of this study was thus to investigate the contribution of miRNAs to the thermal sensitivity of human oral squamous cell carcinoma (OSCC) cells. When the HSC-2, HSC-3 and HSC-4 OSCC cell lines were treated with HT at 44˚C for 60 min, a significant increase in cell death was observed in HSC-2 and HSC-3 cells but not HSC-4 cells, suggesting that HSC-4 cells were thermally resistant under the present experimental conditions. Moreover, the expression levels of HSPs were most elevated in HSC-4 cells. When the basal expression levels of miRNAs were monitored using two different microarray systems in thermal-sensitive HSC-2 and HSC-3 cells and thermal-resistant HSC-4 cells, five miRNAs that were differentially expressed were identified. Among these miRNAs, the expression level of miR-27a in HSC-4 cells was markedly reducec compared to the expression levels in HSC-2 and HSC-3 cells. Interestingly, treatment of HSC-4 cells with a miR-27a mimic oligonucleotide significantly enhanced HT-induced cell death. Furthermore, the miR-27a mimic oligonucleotide suppressed the elevation of the expression of Hsp90 and Hsp110 in HSC-4 cells, suggesting that these HSPs may be involved in a mechanism of thermal resistance. From these findings, we concluded that in OSCC cells, miR-27a may contribute to thermal sensitivity by modulating the HSP expression.
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1 

Hildebrandt B, Wust P, Ahlers O, et al: The cellular and molecular basis of hyperthermia. Crit Rev Oncol Hematol. 43:33–56. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Vos MJ, Hageman J, Carra S and Kampinga HH: Structural and functional diversities between members of the human HSPB, HSPH, HSPA, and DNAJ chaperone families. Biochemistry. 47:7001–7011. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Minton KW, Karmin P, Hahn GM and Minton AP: Nonspecific stabilization of stress-susceptible proteins by stress-resistant proteins: a model for the biological role of heat shock proteins. Proc Natl Acad Sci USA. 79:7107–7111. 1982. View Article : Google Scholar : PubMed/NCBI

4 

Lanneau D, Brunet M, Frisan E, Solary E, Fontenay M and Garrido C: Heat shock proteins: essential proteins for apoptosis regulation. J Cell Mol Med. 12:743–761. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Ciocca DR and Calderwood SK: Heat shock proteins in cancer: diagnostic, prognostic, predictive, and treatment implications. Cell Stress Chaperones. 10:86–103. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Calderwood SK, Khaleque MA, Sawyer DB and Ciocca DR: Heat shock proteins in cancer: chaperones of tumorigenesis. Trends Biochem Sci. 31:164–172. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Filipowicz W, Bhattacharyya SN and Sonenberg N: Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? Nat Rev Genet. 9:102–114. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Meltzer PS: Cancer genomics: small RNAs with big impacts. Nature. 435:745–746. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Krol J, Loedige I and Filipowicz W: The widespread regulation of microRNA biogenesis, function and decay. Nat Rev Genet. 11:597–610. 2010.PubMed/NCBI

10 

Moss EG: MicroRNAs: hidden in the genome. Curr Biol. 12:R138–R140. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Wang J and Cui Q: Specific roles of microRNAs in their interactions with environmental factors. J Nucleic Acids. 2012:9783842012. View Article : Google Scholar : PubMed/NCBI

12 

Niemoeller OM, Niyazi M, Corradini S, et al: MicroRNA expression profiles in human cancer cells after ionizing radiation. Radiat Oncol. 6:292011. View Article : Google Scholar : PubMed/NCBI

13 

Wilmink GJ, Roth CL, Ibey BL, et al: Identification of microRNAs associated with hyperthermia-induced cellular stress response. Cell Stress Chaperones. 15:1027–1038. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Gwak HS, Kim TH, Jo GH, et al: Silencing of microRNA-21 confers radio-sensitivity through inhibition of the PI3K/AKT pathway and enhancing autophagy in malignant glioma cell lines. PLoS One. 7:e474492012. View Article : Google Scholar : PubMed/NCBI

15 

Chistiakov DA and Chekhonin VP: Contribution of microRNAs to radio- and chemoresistance of brain tumors and their therapeutic potential. Eur J Pharmacol. 684:8–18. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Tabuchi Y, Wada S, Furusawa Y, Ohtsuka K and Kondo T: Gene networks related to the cell death elicited by hyperthermia in human oral squamous cell carcinoma HSC-3 cells. Int J Mol Med. 29:380–386. 2012.

17 

Kariya A, Tabuchi Y, Yunoki T and Kondo T: Identification of common gene networks responsive to mild hyperthermia in human cancer cells. Int J Mol Med. 32:195–202. 2013.PubMed/NCBI

18 

Tabuchi Y, Furusawa Y, Kariya A, Wada S, Ohtsuka K and Kondo T: Common gene expression patterns responsive to mild temperature hyperthermia in normal human fibroblastic cells. Int J Hyperthermia. 29:38–50. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Hosaka S, Nakatsura T, Tsukamoto H, Hatayama T, Baba H and Nishimura Y: Synthetic small interfering RNA targeting heat shock protein 105 induces apoptosis of various cancer cells both in vitro and in vivo. Cancer Sci. 97:623–632. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Xu W and Neckers L: Targeting the molecular chaperone heat shock protein 90 provides a multifaceted effect on diverse cell signaling pathways of cancer cells. Clin Cancer Res. 13:1625–1629. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Plaisance-Bonstaff K and Renne R: Viral miRNAs. Methods Mol Biol. 721:43–66. 2011. View Article : Google Scholar

22 

Naito Y and Bono H: GGRNA: an ultrafast, transcript-oriented search engine for genes and transcripts. Nucleic Acids Res. 40:W592–W596. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Cho WS: OncomiRs: the discovery and progress of microRNAs in cancers. Mol Cancer. 6:602007. View Article : Google Scholar : PubMed/NCBI

24 

Kong YW, Ferland-McCollough D, Jackson TJ and Bushell M: MicroRNAs in cancer management. Lancet Oncol. 13:e249–e258. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Guo J, Miao Y, Xiao B, et al: Differential expression of microRNA species in human gastric cancer versus non-tumorous tissues. J Gastroenterol Hepatol. 24:652–657. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Lewis BP, Burge CB and Bartel DP: Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell. 120:15–20. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Ott CE, Grünhagen J, Jäger M, et al: MicroRNAs differentially expressed in postnatal aortic development downregulate elastin via 3′ UTR and coding-sequence binding sites. PLoS One. 6:e162502011.PubMed/NCBI

28 

Oh HJ, Chen X and Subjeck JR: Hsp110 protects heat-denatured proteins and confers cellular thermoresistance. J Biol Chem. 272:31636–31640. 1997. View Article : Google Scholar : PubMed/NCBI

29 

Oh HJ, Easton D, Murawski M, Kaneko Y and Subjeck JR: The chaperoning activity of hsp110. Identification of functional domains by use of targeted deletions. J Biol Chem. 274:15712–15718. 1999. View Article : Google Scholar : PubMed/NCBI

30 

Dorard C, de Thonel A, Collura A, et al: Expression of a mutant HSP110 sensitizes colorectal cancer cells to chemotherapy and improves disease prognosis. Nat Med. 17:1283–1289. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Den RB and Lu B: Heat shock protein 90 inhibition: rationale and clinical potential. Ther Adv Med Oncol. 4:211–218. 2012. View Article : Google Scholar : PubMed/NCBI

32 

Bagatell R and Whitesell L: Altered Hsp90 function in cancer: a unique therapeutic opportunity. Mol Cancer Ther. 3:1021–1030. 2004.PubMed/NCBI

33 

Ito A, Saito H, Mitobe K, et al: Inhibition of heat shock protein 90 sensitizes melanoma cells to thermosensitive ferromagnetic particle-mediated hyperthermia with low Curie temperature. Cancer Sci. 100:558–564. 2009. View Article : Google Scholar

34 

Workman P, Burrows F, Neckers L and Rosen N: Drugging the cancer chaperone HSP90: combinatorial therapeutic exploitation of oncogene addiction and tumor stress. Ann NY Acad Sci. 1113:202–216. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Kariya A, Furusawa Y, Yunoki T, Kondo T and Tabuchi Y: A microRNA-27a mimic sensitizes human oral squamous cell carcinoma HSC-4 cells to hyperthermia through downregulation of Hsp110 and Hsp90. Int J Mol Med 34: 334-340, 2014.
APA
Kariya, A., Furusawa, Y., Yunoki, T., Kondo, T., & Tabuchi, Y. (2014). A microRNA-27a mimic sensitizes human oral squamous cell carcinoma HSC-4 cells to hyperthermia through downregulation of Hsp110 and Hsp90. International Journal of Molecular Medicine, 34, 334-340. https://doi.org/10.3892/ijmm.2014.1758
MLA
Kariya, A., Furusawa, Y., Yunoki, T., Kondo, T., Tabuchi, Y."A microRNA-27a mimic sensitizes human oral squamous cell carcinoma HSC-4 cells to hyperthermia through downregulation of Hsp110 and Hsp90". International Journal of Molecular Medicine 34.1 (2014): 334-340.
Chicago
Kariya, A., Furusawa, Y., Yunoki, T., Kondo, T., Tabuchi, Y."A microRNA-27a mimic sensitizes human oral squamous cell carcinoma HSC-4 cells to hyperthermia through downregulation of Hsp110 and Hsp90". International Journal of Molecular Medicine 34, no. 1 (2014): 334-340. https://doi.org/10.3892/ijmm.2014.1758
Copy and paste a formatted citation
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Spandidos Publications style
Kariya A, Furusawa Y, Yunoki T, Kondo T and Tabuchi Y: A microRNA-27a mimic sensitizes human oral squamous cell carcinoma HSC-4 cells to hyperthermia through downregulation of Hsp110 and Hsp90. Int J Mol Med 34: 334-340, 2014.
APA
Kariya, A., Furusawa, Y., Yunoki, T., Kondo, T., & Tabuchi, Y. (2014). A microRNA-27a mimic sensitizes human oral squamous cell carcinoma HSC-4 cells to hyperthermia through downregulation of Hsp110 and Hsp90. International Journal of Molecular Medicine, 34, 334-340. https://doi.org/10.3892/ijmm.2014.1758
MLA
Kariya, A., Furusawa, Y., Yunoki, T., Kondo, T., Tabuchi, Y."A microRNA-27a mimic sensitizes human oral squamous cell carcinoma HSC-4 cells to hyperthermia through downregulation of Hsp110 and Hsp90". International Journal of Molecular Medicine 34.1 (2014): 334-340.
Chicago
Kariya, A., Furusawa, Y., Yunoki, T., Kondo, T., Tabuchi, Y."A microRNA-27a mimic sensitizes human oral squamous cell carcinoma HSC-4 cells to hyperthermia through downregulation of Hsp110 and Hsp90". International Journal of Molecular Medicine 34, no. 1 (2014): 334-340. https://doi.org/10.3892/ijmm.2014.1758
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