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Article

Sonic hedgehog secreted by neurons regulates angiopoietin expression in neighboring fibroblasts

  • Authors:
    • Woo Jean Kim
    • Sae-Won Lee
    • Kyu-Won Kim
  • View Affiliations / Copyright

    Affiliations: National Research Laboratory of Regenerative Sexual Medicine, Department of Urology, Inha University School of Medicine, Incheon, Republic of Korea, Department of Internal Medicine and Innovative Research Institute for Cell Therapy, Seoul National University Hospital, Seoul, Republic of Korea, Division of Pharmaceutical Biosciences, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Republic of Korea
  • Pages: 213-218
    |
    Published online on: May 2, 2014
       https://doi.org/10.3892/ijmm.2014.1767
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Abstract

Lately, the importance of the communication between different cell types and the understanding of the cell communication pathway has been emphasized, as it may provide a novel therapeutic strategy for the regeneration of damaged tissue. In the present study, we suggest that sonic hedgehog (Shh) is a mediator of cell communication between neurons and fibroblasts. Recombinant Shh (rShh) affected the expression of the angiogenic factors, angiopoietin (Ang)-1 and Ang-2, in fibroblasts, but not in neurons or neural progenitor cells (NPCs). The expression of the Shh downstream transcription factor, Gli1, was markedly increased in neurons and NPCs, indicating that neurons and NPCs responded to rShh. However, rShh did not affect Ang-1 and Ang-2 expression in neurons and NPCs. It should be noted that Shh was strongly expressed in neurons, but that Shh expression was undetectable in fibroblasts. We performed a co-culture assay using neurons and fibroblasts to investigate whether the expression of Ang-1 and Ang-2 is regulated by cell communication, without rShh treatment. Ang-1 expression in fibroblasts was markedly upregulated by co-culture with neurons, whereas Ang-2 expression was decreased by co-culture with neurons. Moreover, when an Shh-neutralizing antibody was added, this effect was diminished. Collectively, our data suggest that Shh-expressing neurons regulate angiopoietin expression in neighboring fibroblasts in a paracrine manner.
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Copy and paste a formatted citation
Spandidos Publications style
Kim WJ, Lee S and Kim K: Sonic hedgehog secreted by neurons regulates angiopoietin expression in neighboring fibroblasts. Int J Mol Med 34: 213-218, 2014.
APA
Kim, W.J., Lee, S., & Kim, K. (2014). Sonic hedgehog secreted by neurons regulates angiopoietin expression in neighboring fibroblasts. International Journal of Molecular Medicine, 34, 213-218. https://doi.org/10.3892/ijmm.2014.1767
MLA
Kim, W. J., Lee, S., Kim, K."Sonic hedgehog secreted by neurons regulates angiopoietin expression in neighboring fibroblasts". International Journal of Molecular Medicine 34.1 (2014): 213-218.
Chicago
Kim, W. J., Lee, S., Kim, K."Sonic hedgehog secreted by neurons regulates angiopoietin expression in neighboring fibroblasts". International Journal of Molecular Medicine 34, no. 1 (2014): 213-218. https://doi.org/10.3892/ijmm.2014.1767
Copy and paste a formatted citation
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Spandidos Publications style
Kim WJ, Lee S and Kim K: Sonic hedgehog secreted by neurons regulates angiopoietin expression in neighboring fibroblasts. Int J Mol Med 34: 213-218, 2014.
APA
Kim, W.J., Lee, S., & Kim, K. (2014). Sonic hedgehog secreted by neurons regulates angiopoietin expression in neighboring fibroblasts. International Journal of Molecular Medicine, 34, 213-218. https://doi.org/10.3892/ijmm.2014.1767
MLA
Kim, W. J., Lee, S., Kim, K."Sonic hedgehog secreted by neurons regulates angiopoietin expression in neighboring fibroblasts". International Journal of Molecular Medicine 34.1 (2014): 213-218.
Chicago
Kim, W. J., Lee, S., Kim, K."Sonic hedgehog secreted by neurons regulates angiopoietin expression in neighboring fibroblasts". International Journal of Molecular Medicine 34, no. 1 (2014): 213-218. https://doi.org/10.3892/ijmm.2014.1767
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