Open Access

Hepatocyte growth factor regulates the TGF‑β1‑induced proliferation, differentiation and secretory function of cardiac fibroblasts

  • Authors:
    • Xin Yi
    • Xiaoyan Li
    • Yanli Zhou
    • Shan Ren
    • Weiguo Wan
    • Gaoke Feng
    • Xuejun Jiang
  • View Affiliations

  • Published online on: May 16, 2014     https://doi.org/10.3892/ijmm.2014.1782
  • Pages: 381-390
  • Copyright: © Yi et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Cardiac fibroblast (CF) proliferation and transformation into myofibroblasts play important roles in cardiac fibrosis during pathological myocardial remodeling. In this study, we demonstrate that hepatocyte growth factor (HGF), an antifibrotic factor in the process of pulmonary, renal and liver fibrosis, is a negative regulator of cardiac fibroblast transformation in response to transforming growth factor‑β1 (TGF‑β1). HGF expression levels were significantly reduced in the CFs following treatment with 5 ng/ml TGF‑β1 for 48 h. The overexpression of HGF suppressed the proliferation, transformation and the secretory function of the CFs following treatment with TGF‑β1, as indicated by the attenuated expression levels of α-smooth muscle actin (α‑SMA) and collagen I and III, whereas the knockdown of HGF had the opposite effect. Mechanistically, we identified that the phosphorylation of c‑Met, Akt and total protein of TGIF was significantly inhibited by the knockdown of HGF, but was significantly enhanced by HGF overexpression. Collectively, these results indicate that HGF activates the c‑Met‑Akt‑TGIF signaling pathway, inhibiting CF proliferation and transformation in response to TGF‑β1 stimulation.
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August-2014
Volume 34 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yi X, Li X, Zhou Y, Ren S, Wan W, Feng G and Jiang X: Hepatocyte growth factor regulates the TGF‑β1‑induced proliferation, differentiation and secretory function of cardiac fibroblasts. Int J Mol Med 34: 381-390, 2014
APA
Yi, X., Li, X., Zhou, Y., Ren, S., Wan, W., Feng, G., & Jiang, X. (2014). Hepatocyte growth factor regulates the TGF‑β1‑induced proliferation, differentiation and secretory function of cardiac fibroblasts. International Journal of Molecular Medicine, 34, 381-390. https://doi.org/10.3892/ijmm.2014.1782
MLA
Yi, X., Li, X., Zhou, Y., Ren, S., Wan, W., Feng, G., Jiang, X."Hepatocyte growth factor regulates the TGF‑β1‑induced proliferation, differentiation and secretory function of cardiac fibroblasts". International Journal of Molecular Medicine 34.2 (2014): 381-390.
Chicago
Yi, X., Li, X., Zhou, Y., Ren, S., Wan, W., Feng, G., Jiang, X."Hepatocyte growth factor regulates the TGF‑β1‑induced proliferation, differentiation and secretory function of cardiac fibroblasts". International Journal of Molecular Medicine 34, no. 2 (2014): 381-390. https://doi.org/10.3892/ijmm.2014.1782